(data stored in ACNUC7421 zone)

SWISSPROT: B2UH26_RALPJ

ID   B2UH26_RALPJ            Unreviewed;       531 AA.
AC   B2UH26;
DT   01-JUL-2008, integrated into UniProtKB/TrEMBL.
DT   01-JUL-2008, sequence version 1.
DT   08-MAY-2019, entry version 84.
DE   RecName: Full=Peptide chain release factor 3 {ECO:0000256|HAMAP-Rule:MF_00072, ECO:0000256|SAAS:SAAS01159250};
DE            Short=RF-3 {ECO:0000256|HAMAP-Rule:MF_00072};
GN   Name=prfC {ECO:0000256|HAMAP-Rule:MF_00072};
GN   OrderedLocusNames=Rpic_3761 {ECO:0000313|EMBL:ACD28877.1};
OS   Ralstonia pickettii (strain 12J).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=402626 {ECO:0000313|EMBL:ACD28877.1, ECO:0000313|Proteomes:UP000002566};
RN   [1] {ECO:0000313|Proteomes:UP000002566}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=12J {ECO:0000313|Proteomes:UP000002566};
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T.,
RA   Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Marsh T., Richardson P.;
RT   "Complete sequence of chromosome 2 of Ralstonia pickettii 12J.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Increases the formation of ribosomal termination
CC       complexes and stimulates activities of RF-1 and RF-2. It binds
CC       guanine nucleotides and has strong preference for UGA stop codons.
CC       It may interact directly with the ribosome. The stimulation of RF-
CC       1 and RF-2 is significantly reduced by GTP and GDP, but not by
CC       GMP. {ECO:0000256|HAMAP-Rule:MF_00072,
CC       ECO:0000256|SAAS:SAAS01159260}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00072,
CC       ECO:0000256|SAAS:SAAS01159242}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. PrfC
CC       subfamily. {ECO:0000256|HAMAP-Rule:MF_00072,
CC       ECO:0000256|SAAS:SAAS01159256}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00072}.
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DR   EMBL; CP001069; ACD28877.1; -; Genomic_DNA.
DR   RefSeq; WP_004632461.1; NC_010678.1.
DR   STRING; 402626.Rpic_3761; -.
DR   EnsemblBacteria; ACD28877; ACD28877; Rpic_3761.
DR   GeneID; 6284993; -.
DR   KEGG; rpi:Rpic_3761; -.
DR   eggNOG; ENOG4105C8A; Bacteria.
DR   eggNOG; COG4108; LUCA.
DR   HOGENOM; HOG000236725; -.
DR   KO; K02837; -.
DR   OMA; VFKIHAN; -.
DR   OrthoDB; 164090at2; -.
DR   Proteomes; UP000002566; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   GO; GO:0006449; P:regulation of translational termination; IEA:UniProtKB-UniRule.
DR   CDD; cd04169; RF3; 1.
DR   Gene3D; 3.30.70.3280; -; 1.
DR   HAMAP; MF_00072; Rel_fac_3; 1.
DR   InterPro; IPR035647; EFG_III/V.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR031157; G_TR_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR004548; PrfC.
DR   InterPro; IPR032090; RF3_C.
DR   InterPro; IPR038467; RF3_C_sf.
DR   InterPro; IPR041732; RF3_GTP-bd.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; TF_GTP-bd_dom.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43556; PTHR43556; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF16658; RF3_C; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF54980; SSF54980; 1.
DR   TIGRFAMs; TIGR00503; prfC; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS00301; G_TR_1; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; B2UH26.
DR   SWISS-2DPAGE; B2UH26.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002566};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00072,
KW   ECO:0000256|SAAS:SAAS01159255};
KW   GTP-binding {ECO:0000256|HAMAP-Rule:MF_00072,
KW   ECO:0000256|SAAS:SAAS01159253};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00072,
KW   ECO:0000256|SAAS:SAAS01159239};
KW   Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00072,
KW   ECO:0000256|SAAS:SAAS01159247};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002566}.
FT   DOMAIN        9    282       Tr-type G. {ECO:0000259|PROSITE:PS51722}.
FT   NP_BIND      90     94       GTP. {ECO:0000256|HAMAP-Rule:MF_00072}.
FT   NP_BIND     144    147       GTP. {ECO:0000256|HAMAP-Rule:MF_00072}.
SQ   SEQUENCE   531 AA;  59212 MW;  AB83A30DE05F66D9 CRC64;
     MSTLQQEIRR RRTFAIIAHP DAGKTTLTEK LLWFGGAIQM AGAVRARKAN RHATSDWMEM
     EKQRGISVTS SVMQFPYRNE GGDYIVNLLD TPGHEDFSED TYRTLTAVDS AVMVIDSVNG
     VEAQTIKLLN VCRLRSTPIL TFINKLDREG RAPIELLDEI ESVLQIQCAP MTWPIGMGKS
     FKGVYHLVND TVQLFDPNAD SEKGATAGLI QGLDNPELDR VLGSQAEELR IDIELVRGAS
     HTFDKEAFLA GKQCPVYFGS AVNNFGVQSL LDALVDQSPE PLARPTETRE VKPMEEKFTG
     FVFKIQANMD PKHRDRIAFV RVCSGRFERG MKLLQVSTGK TVAINNAITF MAQDRNTTEE
     AFAGDIIGVP NHGTIRLGDA FTEGEALKFT GIPSFAPEFF RRARLNNPLR LKQLQKGLQQ
     LAEEGATQMF RPLASNDLVL GAVGILQFDV VAHRLEHEYG VDAIFEPHEC STARWLRGKQ
     EDIDKLIDKA GHNVALDGAG DYVYLAPSQV NLRLTQERFP DIQFMETREI V
//

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