(data stored in ACNUC7421 zone)

SWISSPROT: PYRE_METNO

ID   PYRE_METNO              Reviewed;         196 AA.
AC   B8IT23;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   07-JUN-2017, entry version 51.
DE   RecName: Full=Orotate phosphoribosyltransferase {ECO:0000255|HAMAP-Rule:MF_01208};
DE            Short=OPRT {ECO:0000255|HAMAP-Rule:MF_01208};
DE            Short=OPRTase {ECO:0000255|HAMAP-Rule:MF_01208};
DE            EC=2.4.2.10 {ECO:0000255|HAMAP-Rule:MF_01208};
GN   Name=pyrE {ECO:0000255|HAMAP-Rule:MF_01208};
GN   OrderedLocusNames=Mnod_0034;
OS   Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=460265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 21967 / CNCM I-2342 / ORS 2060;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T.,
RA   Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Ivanova N., Marx C.J., Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium nodulans ORS
RT   2060.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the transfer of a ribosyl phosphate group from
CC       5-phosphoribose 1-diphosphate to orotate, leading to the formation
CC       of orotidine monophosphate (OMP). {ECO:0000255|HAMAP-
CC       Rule:MF_01208}.
CC   -!- CATALYTIC ACTIVITY: Orotidine 5'-phosphate + diphosphate = orotate
CC       + 5-phospho-alpha-D-ribose 1-diphosphate. {ECO:0000255|HAMAP-
CC       Rule:MF_01208}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01208};
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; UMP from orotate: step 1/2. {ECO:0000255|HAMAP-
CC       Rule:MF_01208}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01208}.
CC   -!- SIMILARITY: Belongs to the purine/pyrimidine
CC       phosphoribosyltransferase family. PyrE subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01208}.
DR   EMBL; CP001349; ACL55085.1; -; Genomic_DNA.
DR   RefSeq; WP_012634324.1; NC_011894.1.
DR   ProteinModelPortal; B8IT23; -.
DR   SMR; B8IT23; -.
DR   STRING; 460265.Mnod_0034; -.
DR   EnsemblBacteria; ACL55085; ACL55085; Mnod_0034.
DR   KEGG; mno:Mnod_0034; -.
DR   eggNOG; ENOG4107VCP; Bacteria.
DR   eggNOG; COG0461; LUCA.
DR   HOGENOM; HOG000059806; -.
DR   KO; K00762; -.
DR   OMA; YFQCAKV; -.
DR   OrthoDB; POG091H034Q; -.
DR   UniPathway; UPA00070; UER00119.
DR   Proteomes; UP000008207; Chromosome.
DR   GO; GO:0004588; F:orotate phosphoribosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019856; P:pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR   CDD; cd06223; PRTases_typeI; 1.
DR   HAMAP; MF_01208; PyrE; 1.
DR   InterPro; IPR023031; OPRT.
DR   InterPro; IPR006273; Orotate_PRibTrfase_bac.
DR   InterPro; IPR000836; PRibTrfase_dom.
DR   InterPro; IPR029057; PRTase-like.
DR   Pfam; PF00156; Pribosyltran; 1.
DR   SUPFAM; SSF53271; SSF53271; 1.
DR   TIGRFAMs; TIGR01367; pyrE_Therm; 1.
DR   PROSITE; PS00103; PUR_PYR_PR_TRANSFER; 1.
PE   3: Inferred from homology;
DR   PRODOM; B8IT23.
DR   SWISS-2DPAGE; B8IT23.
KW   Complete proteome; Glycosyltransferase; Magnesium;
KW   Pyrimidine biosynthesis; Reference proteome; Transferase.
FT   CHAIN         1    196       Orotate phosphoribosyltransferase.
FT                                /FTId=PRO_1000164686.
FT   REGION      117    125       5-phosphoribose 1-diphosphate binding.
FT                                {ECO:0000255|HAMAP-Rule:MF_01208}.
FT   BINDING     121    121       Orotate. {ECO:0000255|HAMAP-
FT                                Rule:MF_01208}.
FT   BINDING     149    149       Orotate. {ECO:0000255|HAMAP-
FT                                Rule:MF_01208}.
SQ   SEQUENCE   196 AA;  20315 MW;  E3CD92290CB1BFD7 CRC64;
     MTPDDLLEEF RAAGALLEGH FILSSGLHSA VFLQKMAIFS DPVRTERVCA GLAALIRARY
     GAVDIVVSPA IGGIVPGYET ARALGARAIF VERDPGGPFQ LRRGFSIPAG ARAVMVEDIV
     TTGLSSRECL AALKAEPGEI VGAACLIDRS GGRADLGAPL VALATLDIPN YPADQLPPEL
     AAIPAVKPGS RAMKAS
//

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