(data stored in ACNUC7421 zone)

SWISSPROT: B8IT30_METNO

ID   B8IT30_METNO            Unreviewed;       200 AA.
AC   B8IT30;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   07-JUN-2017, entry version 65.
DE   RecName: Full=3-isopropylmalate dehydratase small subunit {ECO:0000256|HAMAP-Rule:MF_01031};
DE            EC=4.2.1.33 {ECO:0000256|HAMAP-Rule:MF_01031};
DE   AltName: Full=Alpha-IPM isomerase {ECO:0000256|HAMAP-Rule:MF_01031};
DE            Short=IPMI {ECO:0000256|HAMAP-Rule:MF_01031};
DE   AltName: Full=Isopropylmalate isomerase {ECO:0000256|HAMAP-Rule:MF_01031};
GN   Name=leuD {ECO:0000256|HAMAP-Rule:MF_01031};
GN   OrderedLocusNames=Mnod_0041 {ECO:0000313|EMBL:ACL55092.1};
OS   Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=460265 {ECO:0000313|EMBL:ACL55092.1, ECO:0000313|Proteomes:UP000008207};
RN   [1] {ECO:0000313|EMBL:ACL55092.1, ECO:0000313|Proteomes:UP000008207}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 21967 / CNCM I-2342 / ORS 2060
RC   {ECO:0000313|Proteomes:UP000008207};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T.,
RA   Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Ivanova N., Marx C.J., Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium nodulans ORS
RT   2060.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate
CC       and 3-isopropylmalate, via the formation of 2-isopropylmaleate.
CC       {ECO:0000256|HAMAP-Rule:MF_01031, ECO:0000256|SAAS:SAAS00682407}.
CC   -!- CATALYTIC ACTIVITY: (2R,3S)-3-isopropylmalate = (2S)-2-
CC       isopropylmalate. {ECO:0000256|HAMAP-Rule:MF_01031,
CC       ECO:0000256|SAAS:SAAS00682397}.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-
CC       leucine from 3-methyl-2-oxobutanoate: step 2/4.
CC       {ECO:0000256|HAMAP-Rule:MF_01031, ECO:0000256|SAAS:SAAS00682398}.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000256|HAMAP-
CC       Rule:MF_01031, ECO:0000256|SAAS:SAAS00682400}.
CC   -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC       {ECO:0000256|HAMAP-Rule:MF_01031, ECO:0000256|SAAS:SAAS00682390}.
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DR   EMBL; CP001349; ACL55092.1; -; Genomic_DNA.
DR   RefSeq; WP_012634331.1; NC_011894.1.
DR   ProteinModelPortal; B8IT30; -.
DR   STRING; 460265.Mnod_0041; -.
DR   EnsemblBacteria; ACL55092; ACL55092; Mnod_0041.
DR   KEGG; mno:Mnod_0041; -.
DR   eggNOG; ENOG4105MQS; Bacteria.
DR   eggNOG; COG0066; LUCA.
DR   HOGENOM; HOG000222939; -.
DR   KO; K01704; -.
DR   OMA; AFTTHTG; -.
DR   OrthoDB; POG091H02FJ; -.
DR   UniPathway; UPA00048; UER00071.
DR   Proteomes; UP000008207; Chromosome.
DR   GO; GO:0009316; C:3-isopropylmalate dehydratase complex; IEA:InterPro.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-HAMAP.
DR   CDD; cd01577; IPMI_Swivel; 1.
DR   Gene3D; 3.20.19.10; -; 1.
DR   HAMAP; MF_01031; LeuD_type1; 1.
DR   InterPro; IPR004431; 3-IsopropMal_deHydase_ssu.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   InterPro; IPR033940; IPMI_Swivel.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   SUPFAM; SSF52016; SSF52016; 1.
DR   TIGRFAMs; TIGR00171; leuD; 1.
PE   3: Inferred from homology;
DR   PRODOM; B8IT30.
DR   SWISS-2DPAGE; B8IT30.
KW   Amino-acid biosynthesis {ECO:0000256|HAMAP-Rule:MF_01031,
KW   ECO:0000256|SAAS:SAAS00682409};
KW   Branched-chain amino acid biosynthesis {ECO:0000256|HAMAP-
KW   Rule:MF_01031, ECO:0000256|SAAS:SAAS00682405};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008207};
KW   Leucine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01031,
KW   ECO:0000256|SAAS:SAAS00682406};
KW   Lyase {ECO:0000256|HAMAP-Rule:MF_01031, ECO:0000256|SAAS:SAAS00710129,
KW   ECO:0000313|EMBL:ACL55092.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008207}.
FT   DOMAIN        1    122       Aconitase_C. {ECO:0000259|Pfam:PF00694}.
SQ   SEQUENCE   200 AA;  22174 MW;  DA5C993E5451969D CRC64;
     MEKFTVLEGV AAPLRIVNVD TDRIIPKQYL KTIERTGLGK GLFSEMRYRD DGSENPDFVL
     NQPAYRNAKI LVAGDNFGCG SSREHAPWAL ADFGIRCIIS TSFADIFFNN SAKNGILLIT
     VSPEDLEKLF DDAERGANAT LTVDLERQEI RGPDGGVLHF EIDPARKHNL LNGLDEIGLT
     LERAPAIEAY EAKLAERPWA
//

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