(data stored in ACNUC7421 zone)

SWISSPROT: B8I9S6_METNO

ID   B8I9S6_METNO            Unreviewed;       189 AA.
AC   B8I9S6;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   07-JUN-2017, entry version 61.
DE   RecName: Full=Crossover junction endodeoxyribonuclease RuvC {ECO:0000256|HAMAP-Rule:MF_00034, ECO:0000256|SAAS:SAAS00703859};
DE            EC=3.1.22.4 {ECO:0000256|HAMAP-Rule:MF_00034, ECO:0000256|SAAS:SAAS00703873};
DE   AltName: Full=Holliday junction nuclease RuvC {ECO:0000256|HAMAP-Rule:MF_00034};
DE   AltName: Full=Holliday junction resolvase RuvC {ECO:0000256|HAMAP-Rule:MF_00034};
GN   Name=ruvC {ECO:0000256|HAMAP-Rule:MF_00034};
GN   OrderedLocusNames=Mnod_0285 {ECO:0000313|EMBL:ACL55329.1};
OS   Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=460265 {ECO:0000313|EMBL:ACL55329.1, ECO:0000313|Proteomes:UP000008207};
RN   [1] {ECO:0000313|EMBL:ACL55329.1, ECO:0000313|Proteomes:UP000008207}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 21967 / CNCM I-2342 / ORS 2060
RC   {ECO:0000313|Proteomes:UP000008207};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T.,
RA   Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Ivanova N., Marx C.J., Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium nodulans ORS
RT   2060.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Nuclease that resolves Holliday junction intermediates
CC       in genetic recombination. Cleaves the cruciform structure in
CC       supercoiled DNA by nicking to strands with the same polarity at
CC       sites symmetrically opposed at the junction in the homologous arms
CC       and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl
CC       group. {ECO:0000256|HAMAP-Rule:MF_00034,
CC       ECO:0000256|SAAS:SAAS00703870}.
CC   -!- CATALYTIC ACTIVITY: Endonucleolytic cleavage at a junction such as
CC       a reciprocal single-stranded crossover between two homologous DNA
CC       duplexes (Holliday junction). {ECO:0000256|HAMAP-Rule:MF_00034,
CC       ECO:0000256|SAAS:SAAS00703869}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00034};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-
CC       Rule:MF_00034};
CC   -!- SIMILARITY: Belongs to the RuvC family. {ECO:0000256|HAMAP-
CC       Rule:MF_00034, ECO:0000256|SAAS:SAAS00703861}.
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DR   EMBL; CP001349; ACL55329.1; -; Genomic_DNA.
DR   ProteinModelPortal; B8I9S6; -.
DR   STRING; 460265.Mnod_0285; -.
DR   EnsemblBacteria; ACL55329; ACL55329; Mnod_0285.
DR   KEGG; mno:Mnod_0285; -.
DR   eggNOG; ENOG4105NHU; Bacteria.
DR   eggNOG; COG0817; LUCA.
DR   HOGENOM; HOG000012181; -.
DR   KO; K01159; -.
DR   OMA; GLTRCGI; -.
DR   OrthoDB; POG091H0202; -.
DR   Proteomes; UP000008207; Chromosome.
DR   GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-HAMAP.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_00034; RuvC; 1.
DR   InterPro; IPR012337; RNaseH-like_dom.
DR   InterPro; IPR020563; X-over_junc_endoDNase_Mg_BS.
DR   InterPro; IPR002176; X-over_junc_endoDNase_RuvC.
DR   PANTHER; PTHR30194:SF4; PTHR30194:SF4; 1.
DR   Pfam; PF02075; RuvC; 1.
DR   PRINTS; PR00696; RSOLVASERUVC.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   TIGRFAMs; TIGR00228; ruvC; 1.
DR   PROSITE; PS01321; RUVC; 1.
PE   3: Inferred from homology;
DR   PRODOM; B8I9S6.
DR   SWISS-2DPAGE; B8I9S6.
KW   Complete proteome {ECO:0000313|Proteomes:UP000008207};
KW   DNA damage {ECO:0000256|HAMAP-Rule:MF_00034,
KW   ECO:0000256|SAAS:SAAS00703865};
KW   DNA recombination {ECO:0000256|HAMAP-Rule:MF_00034,
KW   ECO:0000256|SAAS:SAAS00703858};
KW   DNA repair {ECO:0000256|HAMAP-Rule:MF_00034,
KW   ECO:0000256|SAAS:SAAS00703862};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00034,
KW   ECO:0000256|SAAS:SAAS00703854, ECO:0000313|EMBL:ACL55329.1};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_00034,
KW   ECO:0000256|SAAS:SAAS00703855};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00034,
KW   ECO:0000256|SAAS:SAAS00703871};
KW   Nuclease {ECO:0000256|HAMAP-Rule:MF_00034,
KW   ECO:0000256|SAAS:SAAS00703860};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008207}.
FT   METAL        13     13       Magnesium. {ECO:0000256|HAMAP-Rule:
FT                                MF_00034}.
FT   METAL        73     73       Magnesium. {ECO:0000256|HAMAP-Rule:
FT                                MF_00034}.
FT   METAL       145    145       Magnesium. {ECO:0000256|HAMAP-Rule:
FT                                MF_00034}.
FT   METAL       148    148       Magnesium. {ECO:0000256|HAMAP-Rule:
FT                                MF_00034}.
SQ   SEQUENCE   189 AA;  19733 MW;  DDEF93FFD9A36D1D CRC64;
     MVTTEPVRIL GIDPGLRRTG WGLITVTGTR LAFVSCGVLT SDGDDDLALR LRDLHDGLSR
     VVAAMQPDEV AVEETFVNKD AQATLKLGHA RAVALLVPAL AGLPVAEYAA NLVKKTVVGA
     GHAEKGQIAA MVRFLLPKAD PATADAADAL AVAITHAQHR AARARELACA APRARPARVG
     RGASLRTRA
//

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