(data stored in ACNUC7421 zone)

SWISSPROT: RP54_SINFN

ID   RP54_SINFN              Reviewed;         519 AA.
AC   P22881; C3MEJ8;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   08-MAY-2019, entry version 105.
DE   RecName: Full=RNA polymerase sigma-54 factor;
GN   Name=rpoN; Synonyms=ntrA; OrderedLocusNames=NGR_c00170;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2211497;
RA   van Slooten J.C., Cervantes E., Broughton W.J., Wong C.H., Stanley J.;
RT   "Sequence and analysis of the rpoN sigma factor gene of rhizobium sp.
RT   strain NGR234, a primary coregulator of symbiosis.";
RL   J. Bacteriol. 172:5563-5574(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/AEM.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of
RT   secretion systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are
CC       then released. This sigma factor is responsible for the expression
CC       of the nitrogen fixation genes (nif operon), glnA and dctA for
CC       dicarboxylate transport. The open complex (sigma-54 and core RNA
CC       polymerase) serves as the receptor for receipt of the melting
CC       signal from the remotely bound activator proteins NifA, NtrC, or
CC       DctD for the expression of the regulated proteins.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
DR   EMBL; M58481; AAA26361.1; -; Genomic_DNA.
DR   EMBL; CP001389; ACP23821.1; -; Genomic_DNA.
DR   PIR; A36130; A36130.
DR   RefSeq; WP_012706606.1; NC_012587.1.
DR   RefSeq; YP_002824574.1; NC_012587.1.
DR   SMR; P22881; -.
DR   STRING; 394.NGR_c00170; -.
DR   PRIDE; P22881; -.
DR   EnsemblBacteria; ACP23821; ACP23821; NGR_c00170.
DR   GeneID; 7790596; -.
DR   KEGG; rhi:NGR_c00170; -.
DR   PATRIC; fig|394.7.peg.2807; -.
DR   eggNOG; ENOG4105DTW; Bacteria.
DR   eggNOG; COG1508; LUCA.
DR   HOGENOM; HOG000071904; -.
DR   KO; K03092; -.
DR   OMA; VTTQKFM; -.
DR   OrthoDB; 1906842at2; -.
DR   Proteomes; UP000001054; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   3: Inferred from homology;
DR   PRODOM; P22881.
DR   SWISS-2DPAGE; P22881.
KW   Complete proteome; DNA-binding; DNA-directed RNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Sigma factor;
KW   Transcription; Transcription regulation; Transferase.
FT   CHAIN         1    519       RNA polymerase sigma-54 factor.
FT                                /FTId=PRO_0000205540.
FT   DNA_BIND    396    415       H-T-H motif. {ECO:0000255}.
FT   MOTIF       487    495       RPON box.
FT   COMPBIAS     11     29       Gln-rich.
FT   CONFLICT     51     53       FQS -> VPI (in Ref. 1; AAA26361).
FT                                {ECO:0000305}.
FT   CONFLICT     58     71       GFGSDRPERDETGL -> VSVRIGPSVMRHV (in Ref.
FT                                1; AAA26361). {ECO:0000305}.
FT   CONFLICT    100    101       ST -> RS (in Ref. 1; AAA26361).
FT                                {ECO:0000305}.
FT   CONFLICT    120    120       Missing (in Ref. 1; AAA26361).
FT                                {ECO:0000305}.
FT   CONFLICT    149    155       FVAGRKT -> SSPVGK (in Ref. 1; AAA26361).
FT                                {ECO:0000305}.
FT   CONFLICT    171    171       P -> A (in Ref. 1; AAA26361).
FT                                {ECO:0000305}.
FT   CONFLICT    202    202       G -> A (in Ref. 1; AAA26361).
FT                                {ECO:0000305}.
FT   CONFLICT    213    213       L -> V (in Ref. 1; AAA26361).
FT                                {ECO:0000305}.
FT   CONFLICT    241    241       R -> G (in Ref. 1; AAA26361).
FT                                {ECO:0000305}.
FT   CONFLICT    257    257       A -> G (in Ref. 1; AAA26361).
FT                                {ECO:0000305}.
FT   CONFLICT    310    310       A -> R (in Ref. 1; AAA26361).
FT                                {ECO:0000305}.
FT   CONFLICT    422    422       I -> R (in Ref. 1; AAA26361).
FT                                {ECO:0000305}.
FT   CONFLICT    514    519       AKAAGF -> PRQPASDCGFFAAAN (in Ref. 1;
FT                                AAA26361). {ECO:0000305}.
SQ   SEQUENCE   519 AA;  57196 MW;  9F46C913E0419375 CRC64;
     MALSASLHLR QSQSLVMTPQ LMQSIQLLQM THLELGQFIA QEVEKNPLLE FQSADEAGFG
     SDRPERDETG LTAEAGGAEE SVDHGDLYDS ATTSPGERLS TELDADFANV FQDDTAPQRA
     DAPELLGQWK SMPGAGGSND GEGYDLDDFV AGRKTLRETL IEQVAFAFAA PADRLIAQHL
     IDQLDEAGYL HAEIAETAAR LGASAADVTR VLLVLQQFDP PGVFARTLSE CLAIQLRLRN
     RLDPAMEALV ANLELLARRD FASLKKICGV DEEDLIEMLA EIRKLDPKPG TSFETSVTEA
     IIPDVVVRSA PDGGWLVELN PDALPRVLVN HDYFAEISRH SQKNSAEQAF LSECMQNANW
     LTRSLDQRAR TILKVASEIV RQQDAFLVHG VDHLRPLNLR IVADAIKMHE STVSRVTSNK
     YILTPRGLFE LKYFFTVSIG SAENGDAHSA ESVRHRIRTM ISQESADAVL SDDDIVDILK
     RAGVDIARRT VAKYREAMNI PSSVQRRREK RALAKAAGF
//

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