(data stored in ACNUC7421 zone)

SWISSPROT: C3MF28_SINFN

ID   C3MF28_SINFN            Unreviewed;       397 AA.
AC   C3MF28;
DT   16-JUN-2009, integrated into UniProtKB/TrEMBL.
DT   16-JUN-2009, sequence version 1.
DT   07-JUN-2017, entry version 60.
DE   RecName: Full=Succinyl-diaminopimelate desuccinylase {ECO:0000256|HAMAP-Rule:MF_01690, ECO:0000256|SAAS:SAAS00765884};
DE            Short=SDAP desuccinylase {ECO:0000256|HAMAP-Rule:MF_01690};
DE            EC=3.5.1.18 {ECO:0000256|HAMAP-Rule:MF_01690};
DE   AltName: Full=N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase {ECO:0000256|HAMAP-Rule:MF_01690};
GN   Name=dapE {ECO:0000256|HAMAP-Rule:MF_01690,
GN   ECO:0000313|EMBL:ACP23865.1};
GN   OrderedLocusNames=NGR_c00610 {ECO:0000313|EMBL:ACP23865.1};
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394 {ECO:0000313|EMBL:ACP23865.1, ECO:0000313|Proteomes:UP000001054};
RN   [1] {ECO:0000313|EMBL:ACP23865.1, ECO:0000313|Proteomes:UP000001054}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234 {ECO:0000313|Proteomes:UP000001054};
RX   PubMed=19376903; DOI=10.1128/AEM.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of
RT   secretion systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: Catalyzes the hydrolysis of N-succinyl-L,L-
CC       diaminopimelic acid (SDAP), forming succinate and LL-2,6-
CC       diaminoheptanedioate (DAP), an intermediate involved in the
CC       bacterial biosynthesis of lysine and meso-diaminopimelic acid, an
CC       essential component of bacterial cell walls.
CC       {ECO:0000256|SAAS:SAAS00765822}.
CC   -!- CATALYTIC ACTIVITY: N-succinyl-LL-2,6-diaminoheptanedioate + H(2)O
CC       = succinate + LL-2,6-diaminoheptanedioate. {ECO:0000256|HAMAP-
CC       Rule:MF_01690, ECO:0000256|SAAS:SAAS00765871}.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP
CC       pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate
CC       (succinylase route): step 3/3. {ECO:0000256|HAMAP-Rule:MF_01690,
CC       ECO:0000256|SAAS:SAAS00765795}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01690,
CC       ECO:0000256|SAAS:SAAS00765883}.
CC   -!- SIMILARITY: Belongs to the peptidase M20A family. DapE subfamily.
CC       {ECO:0000256|HAMAP-Rule:MF_01690, ECO:0000256|SAAS:SAAS00765843}.
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DR   EMBL; CP001389; ACP23865.1; -; Genomic_DNA.
DR   RefSeq; WP_012706650.1; NC_012587.1.
DR   RefSeq; YP_002824618.1; NC_012587.1.
DR   ProteinModelPortal; C3MF28; -.
DR   STRING; 394.NGR_c00610; -.
DR   MEROPS; M20.010; -.
DR   EnsemblBacteria; ACP23865; ACP23865; NGR_c00610.
DR   GeneID; 7790640; -.
DR   KEGG; rhi:NGR_c00610; -.
DR   PATRIC; fig|394.7.peg.2854; -.
DR   eggNOG; ENOG4105C9Y; Bacteria.
DR   eggNOG; COG0624; LUCA.
DR   HOGENOM; HOG000243770; -.
DR   KO; K01439; -.
DR   OMA; LNSHHDT; -.
DR   OrthoDB; POG091H059V; -.
DR   UniPathway; UPA00034; UER00021.
DR   Proteomes; UP000001054; Chromosome.
DR   GO; GO:0050897; F:cobalt ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR   GO; GO:0009014; F:succinyl-diaminopimelate desuccinylase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0019877; P:diaminopimelate biosynthetic process; IEA:UniProtKB-HAMAP.
DR   GO; GO:0009089; P:lysine biosynthetic process via diaminopimelate; IEA:UniProtKB-HAMAP.
DR   CDD; cd03891; M20_DapE_proteobac; 1.
DR   HAMAP; MF_01690; DapE; 1.
DR   InterPro; IPR001261; ArgE/DapE_CS.
DR   InterPro; IPR005941; DapE_proteobac.
DR   InterPro; IPR002933; Peptidase_M20.
DR   InterPro; IPR011650; Peptidase_M20_dimer.
DR   Pfam; PF07687; M20_dimer; 1.
DR   Pfam; PF01546; Peptidase_M20; 1.
DR   SUPFAM; SSF55031; SSF55031; 1.
DR   TIGRFAMs; TIGR01246; dapE_proteo; 1.
DR   PROSITE; PS00758; ARGE_DAPE_CPG2_1; 1.
DR   PROSITE; PS00759; ARGE_DAPE_CPG2_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; C3MF28.
DR   SWISS-2DPAGE; C3MF28.
KW   Amino-acid biosynthesis {ECO:0000256|HAMAP-Rule:MF_01690,
KW   ECO:0000256|SAAS:SAAS00765856};
KW   Cobalt {ECO:0000256|HAMAP-Rule:MF_01690,
KW   ECO:0000256|SAAS:SAAS00765819};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001054};
KW   Diaminopimelate biosynthesis {ECO:0000256|HAMAP-Rule:MF_01690,
KW   ECO:0000256|SAAS:SAAS00765792};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_01690,
KW   ECO:0000256|SAAS:SAAS00786827, ECO:0000313|EMBL:ACP23865.1};
KW   Lysine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01690,
KW   ECO:0000256|SAAS:SAAS00765799};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_01690,
KW   ECO:0000256|SAAS:SAAS00786925};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001054};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_01690, ECO:0000256|SAAS:SAAS00765827}.
FT   DOMAIN      182    288       M20_dimer. {ECO:0000259|Pfam:PF07687}.
FT   ACT_SITE     75     75       {ECO:0000256|HAMAP-Rule:MF_01690}.
FT   ACT_SITE    140    140       Proton acceptor. {ECO:0000256|HAMAP-Rule:
FT                                MF_01690}.
FT   METAL        73     73       Cobalt or zinc 1. {ECO:0000256|HAMAP-
FT                                Rule:MF_01690}.
FT   METAL       106    106       Cobalt or zinc 1. {ECO:0000256|HAMAP-
FT                                Rule:MF_01690}.
FT   METAL       106    106       Cobalt or zinc 2. {ECO:0000256|HAMAP-
FT                                Rule:MF_01690}.
FT   METAL       141    141       Cobalt or zinc 2. {ECO:0000256|HAMAP-
FT                                Rule:MF_01690}.
FT   METAL       169    169       Cobalt or zinc 1. {ECO:0000256|HAMAP-
FT                                Rule:MF_01690}.
FT   METAL       366    366       Cobalt or zinc 2. {ECO:0000256|HAMAP-
FT                                Rule:MF_01690}.
SQ   SEQUENCE   397 AA;  42380 MW;  B592ABB5B41197A7 CRC64;
     MFPTDPISNL AALIRCPSVT PAEGGALTAL EAMLAPLGFS VDRVVAKEAG TPDIENLYAR
     LGAEGPHLMF AGHTDVVPVG DEAAWSHPPF SAAIARGEMY GRGAVDMKGG IACFVAAVAR
     HIEKHGRPKG SISFLITGDE EGPAINGTVK LLEWAAQKGE RWDACLVGEP TNPESLGDMI
     KIGRRGSLSG RITVHGVQGH AAYPHLADNP VRGILQLTQA LMDSAFDAGT DTFQPSNLEV
     TTIDVGNAAV NVIPARASAA FNIRFNDHWT AESLKAEIVA RLDRAAKDET LRPGRQPVKY
     DIVWNERPSH VFLTRNNALI DSLSGAVEAV TGRQPKLSTT GGTSDARFIK DYCPVVEFGL
     VGQTMHMVDE RVAIADLETL TGIYEAFVAR WFGHAAA
//

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