(data stored in ACNUC7421 zone)

SWISSPROT: LPXK_SINFN

ID   LPXK_SINFN              Reviewed;         346 AA.
AC   C3MGV8;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   07-JUN-2017, entry version 45.
DE   RecName: Full=Tetraacyldisaccharide 4'-kinase {ECO:0000255|HAMAP-Rule:MF_00409};
DE            EC=2.7.1.130 {ECO:0000255|HAMAP-Rule:MF_00409};
DE   AltName: Full=Lipid A 4'-kinase {ECO:0000255|HAMAP-Rule:MF_00409};
GN   Name=lpxK {ECO:0000255|HAMAP-Rule:MF_00409};
GN   OrderedLocusNames=NGR_c04270;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/AEM.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of
RT   secretion systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: Transfers the gamma-phosphate of ATP to the 4'-position
CC       of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-
CC       P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).
CC       {ECO:0000255|HAMAP-Rule:MF_00409}.
CC   -!- CATALYTIC ACTIVITY: ATP + (2-N,3-O-bis((3R)-3-
CC       hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-
CC       bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) =
CC       ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-
CC       D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-
CC       alpha-D-glucosaminyl phosphate). {ECO:0000255|HAMAP-
CC       Rule:MF_00409}.
CC   -!- PATHWAY: Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid
CC       IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and
CC       UDP-N-acetyl-alpha-D-glucosamine: step 6/6. {ECO:0000255|HAMAP-
CC       Rule:MF_00409}.
CC   -!- SIMILARITY: Belongs to the LpxK family. {ECO:0000255|HAMAP-
CC       Rule:MF_00409}.
DR   EMBL; CP001389; ACP24223.1; -; Genomic_DNA.
DR   RefSeq; WP_012707008.1; NC_012587.1.
DR   RefSeq; YP_002824976.1; NC_012587.1.
DR   STRING; 394.NGR_c04270; -.
DR   EnsemblBacteria; ACP24223; ACP24223; NGR_c04270.
DR   GeneID; 7791653; -.
DR   KEGG; rhi:NGR_c04270; -.
DR   PATRIC; fig|394.7.peg.3233; -.
DR   eggNOG; ENOG4105CHD; Bacteria.
DR   eggNOG; COG1663; LUCA.
DR   HOGENOM; HOG000004954; -.
DR   KO; K00912; -.
DR   OMA; MDDGFQN; -.
DR   OrthoDB; POG091H000L; -.
DR   UniPathway; UPA00359; UER00482.
DR   Proteomes; UP000001054; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0009029; F:tetraacyldisaccharide 4'-kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-KW.
DR   HAMAP; MF_00409; LpxK; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003758; Tetraacyldisaccharide_4-kinase.
DR   PANTHER; PTHR42724; PTHR42724; 1.
DR   Pfam; PF02606; LpxK; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00682; lpxK; 1.
PE   3: Inferred from homology;
DR   PRODOM; C3MGV8.
DR   SWISS-2DPAGE; C3MGV8.
KW   ATP-binding; Complete proteome; Kinase; Lipid A biosynthesis;
KW   Lipid biosynthesis; Lipid metabolism; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN         1    346       Tetraacyldisaccharide 4'-kinase.
FT                                /FTId=PRO_1000134749.
FT   NP_BIND      54     61       ATP. {ECO:0000255|HAMAP-Rule:MF_00409}.
SQ   SEQUENCE   346 AA;  37317 MW;  614DE64B69947D52 CRC64;
     MVSEAPPFWW TKADWRAYAL WPFAWAYGRV AAIRMDRARR AMPPVPLICV GNFTVGGAGK
     TPTAIALAQA AKARGLKPGF LSRGYGGSLD VTTVVDPGHH RARDVGDEPL LLAREALTVV
     CRRRVDGARR LAAEGADIII MDDGFQSARL TFDFALLVID SMRGIGNGHL VPSGPVRAPI
     ADQLRHATAL LKIGHGPAAD RLIRRAARAG KAIYVADTVR LDDGSMSGVK VLAWAGIADP
     EKFYRTVRDA GAIVEETRSF PDHHHFSDDE IADLIDRAAS QGYTLVTTAK DMVRLEPGHG
     RAAELAAKSR VIEIEVRFDD PLAPAKIIDA TLAAARARKL RANGKG
//

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