(data stored in ACNUC7421 zone)

SWISSPROT: RNH2_SINFN

ID   RNH2_SINFN              Reviewed;         231 AA.
AC   C3MHC0;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   08-MAY-2019, entry version 59.
DE   RecName: Full=Ribonuclease HII {ECO:0000255|HAMAP-Rule:MF_00052};
DE            Short=RNase HII {ECO:0000255|HAMAP-Rule:MF_00052};
DE            EC=3.1.26.4 {ECO:0000255|HAMAP-Rule:MF_00052};
GN   Name=rnhB {ECO:0000255|HAMAP-Rule:MF_00052};
GN   OrderedLocusNames=NGR_c04520;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/AEM.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of
RT   secretion systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: Endonuclease that specifically degrades the RNA of RNA-
CC       DNA hybrids. {ECO:0000255|HAMAP-Rule:MF_00052}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.;
CC         EC=3.1.26.4; Evidence={ECO:0000255|HAMAP-Rule:MF_00052};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00052};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00052};
CC       Note=Manganese or magnesium. Binds 1 divalent metal ion per
CC       monomer in the absence of substrate. May bind a second metal ion
CC       after substrate binding. {ECO:0000255|HAMAP-Rule:MF_00052};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00052}.
CC   -!- SIMILARITY: Belongs to the RNase HII family. {ECO:0000255|HAMAP-
CC       Rule:MF_00052}.
DR   EMBL; CP001389; ACP24248.1; -; Genomic_DNA.
DR   RefSeq; WP_012707033.1; NC_012587.1.
DR   RefSeq; YP_002825001.1; NC_012587.1.
DR   SMR; C3MHC0; -.
DR   STRING; 394.NGR_c04520; -.
DR   EnsemblBacteria; ACP24248; ACP24248; NGR_c04520.
DR   GeneID; 7791678; -.
DR   KEGG; rhi:NGR_c04520; -.
DR   PATRIC; fig|394.7.peg.3259; -.
DR   eggNOG; ENOG4108UH2; Bacteria.
DR   eggNOG; COG0164; LUCA.
DR   HOGENOM; HOG000100288; -.
DR   KO; K03470; -.
DR   OMA; NILHASM; -.
DR   OrthoDB; 1685277at2; -.
DR   Proteomes; UP000001054; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0004523; F:RNA-DNA hybrid ribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd07182; RNase_HII_bacteria_HII_like; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_00052_B; RNase_HII_B; 1.
DR   InterPro; IPR022898; RNase_HII.
DR   InterPro; IPR001352; RNase_HII/HIII.
DR   InterPro; IPR024567; RNase_HII/HIII_dom.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR10954; PTHR10954; 1.
DR   PANTHER; PTHR10954:SF18; PTHR10954:SF18; 1.
DR   Pfam; PF01351; RNase_HII; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   3: Inferred from homology;
DR   PRODOM; C3MHC0.
DR   SWISS-2DPAGE; C3MHC0.
KW   Complete proteome; Cytoplasm; Endonuclease; Hydrolase; Manganese;
KW   Metal-binding; Nuclease; Reference proteome.
FT   CHAIN         1    231       Ribonuclease HII.
FT                                /FTId=PRO_1000117680.
FT   METAL        39     39       Divalent metal cation.
FT                                {ECO:0000255|HAMAP-Rule:MF_00052}.
FT   METAL        40     40       Divalent metal cation.
FT                                {ECO:0000255|HAMAP-Rule:MF_00052}.
FT   METAL       130    130       Divalent metal cation.
FT                                {ECO:0000255|HAMAP-Rule:MF_00052}.
SQ   SEQUENCE   231 AA;  24428 MW;  1273A1F4997CC562 CRC64;
     MSRRKSPDSP LFQLEPVLPD FSFEITAKRD GFWPVAGADE AGRGPLAGPV VAAAVILDPD
     AVPGGLNDSK LLTAEQREAL FTEIMATATV SIASSSAAHI DATDILKASL DAMRRAIDGL
     SIAARFALID GRDVPQGLPC HAKAIVKGDS RSVSIAAASI VAKVTRDRMM ARADATFPAY
     GFALHAGYAT VRHRSAIDSH GPCSLHRMSF RPFREAQEQP LAVSTGPIDP N
//

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