(data stored in ACNUC7421 zone)

SWISSPROT: TUSA_EDWI9

ID   TUSA_EDWI9              Reviewed;          82 AA.
AC   C5B9A2;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   07-JUN-2017, entry version 47.
DE   RecName: Full=Sulfur carrier protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=Sulfur mediator TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=Sulfur transfer protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000255|HAMAP-Rule:MF_00413};
GN   Name=tusA {ECO:0000255|HAMAP-Rule:MF_00413};
GN   OrderedLocusNames=NT01EI_0022;
OS   Edwardsiella ictaluri (strain 93-146).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Hafniaceae; Edwardsiella.
OX   NCBI_TaxID=634503;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146;
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.;
RT   "Complete genome sequence of Edwardsiella ictaluri 93-146.";
RL   Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in
CC       the cell. Part of a sulfur-relay system required for 2-thiolation
CC       during synthesis of 2-thiouridine of the modified wobble base 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts
CC       with IscS and stimulates its cysteine desulfurase activity.
CC       Accepts an activated sulfur from IscS, which is then transferred
CC       to TusD, and thus determines the direction of sulfur flow from
CC       IscS to 2-thiouridine formation. Also appears to be involved in
CC       sulfur transfer for the biosynthesis of molybdopterin.
CC       {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- PATHWAY: tRNA modification. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SUBUNIT: Interacts with IscS. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC       {ECO:0000255|HAMAP-Rule:MF_00413}.
DR   EMBL; CP001600; ACR67282.1; -; Genomic_DNA.
DR   RefSeq; WP_015869506.1; NC_012779.2.
DR   ProteinModelPortal; C5B9A2; -.
DR   SMR; C5B9A2; -.
DR   STRING; 634503.NT01EI_0022; -.
DR   EnsemblBacteria; ACR67282; ACR67282; NT01EI_0022.
DR   GeneID; 7959341; -.
DR   KEGG; eic:NT01EI_0022; -.
DR   PATRIC; fig|634503.3.peg.18; -.
DR   eggNOG; ENOG41082K8; Bacteria.
DR   eggNOG; COG0425; LUCA.
DR   HOGENOM; HOG000259291; -.
DR   KO; K04085; -.
DR   OMA; DAQGLRC; -.
DR   OrthoDB; POG091H06BL; -.
DR   Proteomes; UP000001485; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0097163; F:sulfur carrier activity; IEA:InterPro.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR   CDD; cd03423; SirA; 1.
DR   Gene3D; 3.30.110.40; -; 1.
DR   HAMAP; MF_00413; Thiourid_synth_A; 1.
DR   InterPro; IPR022931; Sulphur_carrier_TusA.
DR   InterPro; IPR001455; TusA-like.
DR   Pfam; PF01206; TusA; 1.
DR   SUPFAM; SSF64307; SSF64307; 1.
DR   PROSITE; PS01148; UPF0033; 1.
PE   3: Inferred from homology;
DR   PRODOM; C5B9A2.
DR   SWISS-2DPAGE; C5B9A2.
KW   Complete proteome; Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN         1     82       Sulfur carrier protein TusA.
FT                                /FTId=PRO_1000206006.
FT   ACT_SITE     19     19       Cysteine persulfide intermediate.
FT                                {ECO:0000255|HAMAP-Rule:MF_00413}.
SQ   SEQUENCE   82 AA;  9262 MW;  D2EF3A259166D52A CRC64;
     MTDLFANPDK TLDALGLRCP EPVMMVRKTV RHMVAGETLL IIADDPATTR DIPGFCRFME
     HTLLAQEIEQ TPYRYLLRKG GA
//

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