(data stored in ACNUC7421 zone)

SWISSPROT: TRMB_EDWI9

ID   TRMB_EDWI9              Reviewed;         238 AA.
AC   C5BCF2;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   07-JUN-2017, entry version 50.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01057};
DE            EC=2.1.1.33 {ECO:0000255|HAMAP-Rule:MF_01057};
DE   AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01057};
DE   AltName: Full=tRNA(m7G46)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01057};
GN   Name=trmB {ECO:0000255|HAMAP-Rule:MF_01057};
GN   OrderedLocusNames=NT01EI_0273;
OS   Edwardsiella ictaluri (strain 93-146).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Hafniaceae; Edwardsiella.
OX   NCBI_TaxID=634503;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146;
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.;
RT   "Complete genome sequence of Edwardsiella ictaluri 93-146.";
RL   Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at
CC       position 46 (m7G46) in tRNA. {ECO:0000255|HAMAP-Rule:MF_01057}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + guanine(46) in tRNA
CC       = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.
CC       {ECO:0000255|HAMAP-Rule:MF_01057}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_01057}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01057}.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding
CC       methyltransferase superfamily. TrmB family. {ECO:0000255|HAMAP-
CC       Rule:MF_01057}.
DR   EMBL; CP001600; ACR67515.1; -; Genomic_DNA.
DR   RefSeq; WP_015869724.1; NC_012779.2.
DR   ProteinModelPortal; C5BCF2; -.
DR   SMR; C5BCF2; -.
DR   STRING; 634503.NT01EI_0273; -.
DR   EnsemblBacteria; ACR67515; ACR67515; NT01EI_0273.
DR   GeneID; 7960053; -.
DR   KEGG; eic:NT01EI_0273; -.
DR   PATRIC; fig|634503.3.peg.245; -.
DR   eggNOG; ENOG4105CZ1; Bacteria.
DR   eggNOG; COG0220; LUCA.
DR   HOGENOM; HOG000073968; -.
DR   KO; K03439; -.
DR   OMA; PDPWHKS; -.
DR   OrthoDB; POG091H015P; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000001485; Chromosome.
DR   GO; GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-EC.
DR   HAMAP; MF_01057; tRNA_methyltr_TrmB; 1.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase_Trmb.
DR   PANTHER; PTHR23417; PTHR23417; 1.
DR   Pfam; PF02390; Methyltransf_4; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR00091; TIGR00091; 1.
DR   PROSITE; PS51625; SAM_MT_TRMB; 1.
PE   3: Inferred from homology;
DR   PRODOM; C5BCF2.
DR   SWISS-2DPAGE; C5BCF2.
KW   Complete proteome; Methyltransferase; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase; tRNA processing.
FT   CHAIN         1    238       tRNA (guanine-N(7)-)-methyltransferase.
FT                                /FTId=PRO_1000213439.
FT   REGION      216    219       Substrate binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_01057}.
FT   ACT_SITE    143    143       {ECO:0000250}.
FT   BINDING      68     68       S-adenosyl-L-methionine.
FT                                {ECO:0000255|HAMAP-Rule:MF_01057}.
FT   BINDING      93     93       S-adenosyl-L-methionine.
FT                                {ECO:0000255|HAMAP-Rule:MF_01057}.
FT   BINDING     120    120       S-adenosyl-L-methionine.
FT                                {ECO:0000255|HAMAP-Rule:MF_01057}.
FT   BINDING     143    143       S-adenosyl-L-methionine.
FT                                {ECO:0000255|HAMAP-Rule:MF_01057}.
FT   BINDING     147    147       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_01057}.
FT   BINDING     179    179       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_01057}.
SQ   SEQUENCE   238 AA;  27086 MW;  BB6313E76A4A4802 CRC64;
     MNDVITAEFD ENGRAIRRIR SFVRRQGRLT KGQQLALDEY WPLMGVEFTP EALDLPTLFG
     REAPVVLEIG FGMGASLVAM AQQHPERDFL GIEVHSPGVG ACLSSAHEAG VNNLRVMCHD
     AVEVLERMIP DASLDMVQLF FPDPWHKARH HKRRIVQSAF AGRIRGKLKI SGVFHMATDW
     ENYAEHMLEV MNHAPGYRNL SDDNTYVPRP DSRPVTKFEL RGQRLGHGNW DLMFERVE
//

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