(data stored in ACNUC7421 zone)

SWISSPROT: C5BDN0_EDWI9

ID   C5BDN0_EDWI9            Unreviewed;       317 AA.
AC   C5BDN0;
DT   28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT   28-JUL-2009, sequence version 1.
DT   07-JUN-2017, entry version 57.
DE   RecName: Full=tRNA dimethylallyltransferase {ECO:0000256|HAMAP-Rule:MF_00185};
DE            EC=2.5.1.75 {ECO:0000256|HAMAP-Rule:MF_00185};
DE   AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase {ECO:0000256|HAMAP-Rule:MF_00185};
DE            Short=DMAPP:tRNA dimethylallyltransferase {ECO:0000256|HAMAP-Rule:MF_00185};
DE            Short=DMATase {ECO:0000256|HAMAP-Rule:MF_00185};
DE   AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase {ECO:0000256|HAMAP-Rule:MF_00185};
DE            Short=IPP transferase {ECO:0000256|HAMAP-Rule:MF_00185};
DE            Short=IPPT {ECO:0000256|HAMAP-Rule:MF_00185};
DE            Short=IPTase {ECO:0000256|HAMAP-Rule:MF_00185};
GN   Name=miaA {ECO:0000256|HAMAP-Rule:MF_00185};
GN   OrderedLocusNames=NT01EI_0408 {ECO:0000313|EMBL:ACR67646.1};
OS   Edwardsiella ictaluri (strain 93-146).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Hafniaceae; Edwardsiella.
OX   NCBI_TaxID=634503 {ECO:0000313|EMBL:ACR67646.1, ECO:0000313|Proteomes:UP000001485};
RN   [1] {ECO:0000313|Proteomes:UP000001485}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146 {ECO:0000313|Proteomes:UP000001485};
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.;
RT   "Complete genome sequence of Edwardsiella ictaluri 93-146.";
RL   Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the
CC       adenine at position 37 in tRNAs that read codons beginning with
CC       uridine, leading to the formation of N6-(dimethylallyl)adenosine
CC       (i(6)A). {ECO:0000256|HAMAP-Rule:MF_00185,
CC       ECO:0000256|RuleBase:RU003784}.
CC   -!- CATALYTIC ACTIVITY: Dimethylallyl diphosphate + adenine(37) in
CC       tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.
CC       {ECO:0000256|HAMAP-Rule:MF_00185, ECO:0000256|RuleBase:RU003783}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00185};
CC   -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00185}.
CC   -!- SIMILARITY: Belongs to the IPP transferase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00185, ECO:0000256|RuleBase:RU003785}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00185}.
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DR   EMBL; CP001600; ACR67646.1; -; Genomic_DNA.
DR   RefSeq; WP_015869852.1; NC_012779.2.
DR   ProteinModelPortal; C5BDN0; -.
DR   STRING; 634503.NT01EI_0408; -.
DR   EnsemblBacteria; ACR67646; ACR67646; NT01EI_0408.
DR   GeneID; 7961233; -.
DR   KEGG; eic:NT01EI_0408; -.
DR   PATRIC; fig|634503.3.peg.368; -.
DR   eggNOG; ENOG4105DKX; Bacteria.
DR   eggNOG; COG0324; LUCA.
DR   HOGENOM; HOG000039996; -.
DR   KO; K00791; -.
DR   OMA; GTGLYIR; -.
DR   OrthoDB; POG091H00NK; -.
DR   Proteomes; UP000001485; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0052381; F:tRNA dimethylallyltransferase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_00185; IPP_trans; 1.
DR   InterPro; IPR018022; IPP_trans.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11088:SF43; PTHR11088:SF43; 1.
DR   Pfam; PF01715; IPPT; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00174; miaA; 1.
PE   3: Inferred from homology;
DR   PRODOM; C5BDN0.
DR   SWISS-2DPAGE; C5BDN0.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00185,
KW   ECO:0000256|RuleBase:RU003785};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001485};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_00185};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00185,
KW   ECO:0000256|RuleBase:RU003785};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001485};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00185,
KW   ECO:0000256|RuleBase:RU003785, ECO:0000313|EMBL:ACR67646.1};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00185,
KW   ECO:0000256|RuleBase:RU003783}.
FT   NP_BIND      18     25       ATP. {ECO:0000256|HAMAP-Rule:MF_00185}.
FT   REGION       20     25       Substrate binding. {ECO:0000256|HAMAP-
FT                                Rule:MF_00185}.
FT   REGION       43     46       Interaction with substrate tRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00185}.
FT   REGION      167    171       Interaction with substrate tRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00185}.
FT   REGION      248    253       Interaction with substrate tRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00185}.
FT   SITE        109    109       Interaction with substrate tRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00185}.
FT   SITE        131    131       Interaction with substrate tRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00185}.
SQ   SEQUENCE   317 AA;  35480 MW;  EE22BF056459B994 CRC64;
     MSNDKQMQKL PPAIFIMGPT ASGKTALAMA LRERFPVEII SVDSALIYRG MDIGTAKPSA
     EELARAPHRL IDIRSPTQSY SAADFRADAL REMAQISAQG RIPLLVGGTM LYYKALLEGL
     SPLPPADDAV RARIEQQASE QGWEVLHREL ARIDPVAAAR IHPNDPQRLS RALEVFFVSG
     NTLTELTRVS GEALPYAVSQ FAIAPSRREL LHQRIALRFQ QMLEAGFEQE ARRLFARDDL
     HPDLPSVRCV GYRQMWSYLA GEIDYDEMVY RGVCATRQLA KRQMTWLRGW ESVQWLDSDK
     PAEALDCVAQ VVSASLD
//

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