(data stored in ACNUC7421 zone)

SWISSPROT: C5BFB9_EDWI9

ID   C5BFB9_EDWI9            Unreviewed;       320 AA.
AC   C5BFB9;
DT   28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT   28-JUL-2009, sequence version 1.
DT   07-JUN-2017, entry version 59.
DE   RecName: Full=tRNA pseudouridine synthase B {ECO:0000256|HAMAP-Rule:MF_01080};
DE            EC=5.4.99.25 {ECO:0000256|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000256|HAMAP-Rule:MF_01080};
DE            Short=Psi55 synthase {ECO:0000256|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridylate synthase {ECO:0000256|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA-uridine isomerase {ECO:0000256|HAMAP-Rule:MF_01080};
GN   Name=truB {ECO:0000256|HAMAP-Rule:MF_01080};
GN   OrderedLocusNames=NT01EI_0469 {ECO:0000313|EMBL:ACR67705.1};
OS   Edwardsiella ictaluri (strain 93-146).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Hafniaceae; Edwardsiella.
OX   NCBI_TaxID=634503 {ECO:0000313|EMBL:ACR67705.1, ECO:0000313|Proteomes:UP000001485};
RN   [1] {ECO:0000313|Proteomes:UP000001485}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146 {ECO:0000313|Proteomes:UP000001485};
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.;
RT   "Complete genome sequence of Edwardsiella ictaluri 93-146.";
RL   Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-
CC       55 in the psi GC loop of transfer RNAs. {ECO:0000256|HAMAP-
CC       Rule:MF_01080, ECO:0000256|SAAS:SAAS00534152}.
CC   -!- CATALYTIC ACTIVITY: tRNA uridine(55) = tRNA pseudouridine(55).
CC       {ECO:0000256|HAMAP-Rule:MF_01080, ECO:0000256|SAAS:SAAS00415305}.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family.
CC       Type 1 subfamily. {ECO:0000256|HAMAP-Rule:MF_01080,
CC       ECO:0000256|SAAS:SAAS00534157}.
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DR   EMBL; CP001600; ACR67705.1; -; Genomic_DNA.
DR   RefSeq; WP_015869907.1; NC_012779.2.
DR   STRING; 634503.NT01EI_0469; -.
DR   EnsemblBacteria; ACR67705; ACR67705; NT01EI_0469.
DR   GeneID; 7961292; -.
DR   KEGG; eic:NT01EI_0469; -.
DR   PATRIC; fig|634503.3.peg.426; -.
DR   eggNOG; ENOG4105D0T; Bacteria.
DR   eggNOG; COG0130; LUCA.
DR   HOGENOM; HOG000231223; -.
DR   KO; K03177; -.
DR   OMA; ELQFIRW; -.
DR   OrthoDB; POG091H02G9; -.
DR   Proteomes; UP000001485; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-HAMAP.
DR   Gene3D; 2.30.130.10; -; 1.
DR   HAMAP; MF_01080; TruB_bact; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR002478; PUA.
DR   InterPro; IPR015947; PUA-like_domain.
DR   InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR   InterPro; IPR015240; tRNA_sdUridine_synth_fam1_C.
DR   InterPro; IPR032819; TruB_C.
DR   PANTHER; PTHR13767; PTHR13767; 1.
DR   Pfam; PF09157; TruB-C_2; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00431; TruB; 1.
PE   3: Inferred from homology;
DR   PRODOM; C5BFB9.
DR   SWISS-2DPAGE; C5BFB9.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001485};
KW   Isomerase {ECO:0000256|HAMAP-Rule:MF_01080,
KW   ECO:0000256|SAAS:SAAS00103062, ECO:0000313|EMBL:ACR67705.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001485};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_01080,
KW   ECO:0000256|SAAS:SAAS00103090}.
FT   DOMAIN       33    180       TruB_N. {ECO:0000259|Pfam:PF01509}.
FT   DOMAIN      181    247       TruB_C_2. {ECO:0000259|Pfam:PF16198}.
FT   DOMAIN      252    309       TruB-C_2. {ECO:0000259|Pfam:PF09157}.
FT   ACT_SITE     48     48       Nucleophile. {ECO:0000256|HAMAP-Rule:
FT                                MF_01080}.
FT   BINDING      43     43       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_01080}.
FT   BINDING      76     76       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_01080}.
FT   BINDING     179    179       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_01080}.
FT   BINDING     200    200       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_01080}.
SQ   SEQUENCE   320 AA;  35618 MW;  D64EC5DCD44F9514 CRC64;
     MSRPRRRGRD INGVLLLDKG QGISSNDVLQ KVKRIYNANK AGHTGALDPL ATGMLPICLG
     EATKFSQFLL DADKRYRVIA RLGQRTDTSD ADGQVVCERP LTFSEAELAQ ALAGFRGPGR
     QVPSMYSALK YQGRPLYEYA RQGIDVPRES REITVFELRF IRWEGDELEL EIHCSKGTYI
     RTIIDDLGEV LGCGAHVIYL RRLQVSGYPS ERMVTLEQLQ VLLAQAQQQE RDPRLLLDPL
     LLPMDTAAAH LPEVNLSEIV AGYVQLGQSV QTPNLPQSGM VRISVGEPHR FIGVGEVDEQ
     GRLAPRRMVV TSRLSEDDPA
//

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