(data stored in ACNUC7421 zone)

SWISSPROT: RF3_EDWI9

ID   RF3_EDWI9               Reviewed;         529 AA.
AC   C5BHI4;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   30-AUG-2017, entry version 52.
DE   RecName: Full=Peptide chain release factor 3 {ECO:0000255|HAMAP-Rule:MF_00072};
DE            Short=RF-3 {ECO:0000255|HAMAP-Rule:MF_00072};
GN   Name=prfC {ECO:0000255|HAMAP-Rule:MF_00072};
GN   OrderedLocusNames=NT01EI_0554;
OS   Edwardsiella ictaluri (strain 93-146).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Hafniaceae; Edwardsiella.
OX   NCBI_TaxID=634503;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146;
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.;
RT   "Complete genome sequence of Edwardsiella ictaluri 93-146.";
RL   Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Increases the formation of ribosomal termination
CC       complexes and stimulates activities of RF-1 and RF-2. It binds
CC       guanine nucleotides and has strong preference for UGA stop codons.
CC       It may interact directly with the ribosome. The stimulation of RF-
CC       1 and RF-2 is significantly reduced by GTP and GDP, but not by
CC       GMP. {ECO:0000255|HAMAP-Rule:MF_00072}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00072}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. PrfC
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00072}.
DR   EMBL; CP001600; ACR67783.1; -; Genomic_DNA.
DR   RefSeq; WP_015869983.1; NC_012779.2.
DR   STRING; 634503.NT01EI_0554; -.
DR   EnsemblBacteria; ACR67783; ACR67783; NT01EI_0554.
DR   GeneID; 7961575; -.
DR   KEGG; eic:NT01EI_0554; -.
DR   PATRIC; fig|634503.3.peg.500; -.
DR   eggNOG; ENOG4105C8A; Bacteria.
DR   eggNOG; COG4108; LUCA.
DR   HOGENOM; HOG000236725; -.
DR   KO; K02837; -.
DR   OMA; VFKIHAN; -.
DR   OrthoDB; POG091H00HD; -.
DR   Proteomes; UP000001485; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:InterPro.
DR   HAMAP; MF_00072; Rel_fac_3; 1.
DR   InterPro; IPR035647; EFG_III/V.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR031157; G_TR_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR004548; PrfC.
DR   InterPro; IPR032090; RF3_C.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; TF_GTP-bd_dom.
DR   InterPro; IPR009000; Transl_B-barrel.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF16658; RF3_C; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF54980; SSF54980; 1.
DR   TIGRFAMs; TIGR00503; prfC; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS00301; G_TR_1; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; C5BHI4.
DR   SWISS-2DPAGE; C5BHI4.
KW   Complete proteome; Cytoplasm; GTP-binding; Nucleotide-binding;
KW   Protein biosynthesis; Reference proteome.
FT   CHAIN         1    529       Peptide chain release factor 3.
FT                                /FTId=PRO_1000202467.
FT   DOMAIN       11    280       tr-type G.
FT   NP_BIND      20     27       GTP. {ECO:0000255|HAMAP-Rule:MF_00072}.
FT   NP_BIND      88     92       GTP. {ECO:0000255|HAMAP-Rule:MF_00072}.
FT   NP_BIND     142    145       GTP. {ECO:0000255|HAMAP-Rule:MF_00072}.
SQ   SEQUENCE   529 AA;  59638 MW;  201119F7236951E9 CRC64;
     MSPSEYAREV AKRRTFAIIS HPDAGKTTIT EKVLLFGQAI QVAGTVKGRG SSQHAKSDWM
     EMEKQRGISI TTSVMQFPYH DCLVNLLDTP GHEDFSEDTY RTLTAVDCCL MVIDAAKGVE
     DRTRKLMEVT RLRDTPILTF MNKLDRDIRD PMELLDEVES ELRIACAPIT WPIGCGKLFK
     GVYHLYKDET YLYQSGKGHT IQEVRVIKGL DNPLLDTAVG EDLAAQLREE LELVQGASTE
     FEEEAFLNGQ LTPVFFGTAL GNFGVDHMLD GLVAWAPAPM PRMTDVRKVS ADEEKFSGFV
     FKIQANMDPK HRDRVAFMRV VSGRYDKGMK LRQVRTGKDV VIADALTFMA GDRAHVEEAY
     PGDIIGLHNH GTIQIGDTFT QGEEMKFTGI PNFAPELFRR IRLRDPLKQK QLLKGLVQLS
     EEGAVQVFRP VANNDLIVGA VGVLQFDVVV ARLKSEYNVE AIYESVNVST ARWVECDDVK
     KFDEFQRKNE LHLALDGGDN LAYIAPTMVN LNLTQERYPE VRFRKTREH
//

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