(data stored in ACNUC7421 zone)

SWISSPROT: C5B7N8_EDWI9

ID   C5B7N8_EDWI9            Unreviewed;       160 AA.
AC   C5B7N8;
DT   28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT   28-JUL-2009, sequence version 1.
DT   07-JUN-2017, entry version 54.
DE   RecName: Full=Dihydrofolate reductase {ECO:0000256|PIRNR:PIRNR000194};
DE            EC=1.5.1.3 {ECO:0000256|PIRNR:PIRNR000194};
GN   OrderedLocusNames=NT01EI_0693 {ECO:0000313|EMBL:ACR67915.1};
OS   Edwardsiella ictaluri (strain 93-146).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Hafniaceae; Edwardsiella.
OX   NCBI_TaxID=634503 {ECO:0000313|EMBL:ACR67915.1, ECO:0000313|Proteomes:UP000001485};
RN   [1] {ECO:0000313|Proteomes:UP000001485}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146 {ECO:0000313|Proteomes:UP000001485};
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.;
RT   "Complete genome sequence of Edwardsiella ictaluri 93-146.";
RL   Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Key enzyme in folate metabolism. Catalyzes an essential
CC       reaction for de novo glycine and purine synthesis, and for DNA
CC       precursor synthesis. {ECO:0000256|PIRNR:PIRNR000194}.
CC   -!- CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-
CC       dihydrofolate + NADPH. {ECO:0000256|PIRNR:PIRNR000194}.
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis;
CC       5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR000194}.
CC   -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC       {ECO:0000256|PIRNR:PIRNR000194, ECO:0000256|RuleBase:RU004474}.
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DR   EMBL; CP001600; ACR67915.1; -; Genomic_DNA.
DR   RefSeq; WP_015870108.1; NC_012779.2.
DR   STRING; 634503.NT01EI_0693; -.
DR   EnsemblBacteria; ACR67915; ACR67915; NT01EI_0693.
DR   GeneID; 7962027; -.
DR   KEGG; eic:NT01EI_0693; -.
DR   PATRIC; fig|634503.3.peg.624; -.
DR   eggNOG; ENOG4108YYV; Bacteria.
DR   eggNOG; COG0262; LUCA.
DR   HOGENOM; HOG000040233; -.
DR   KO; K00287; -.
DR   OMA; RDNQLPW; -.
DR   OrthoDB; POG091H01KY; -.
DR   UniPathway; UPA00077; UER00158.
DR   Proteomes; UP000001485; Chromosome.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR   GO; GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00209; DHFR; 1.
DR   Gene3D; 3.40.430.10; -; 1.
DR   InterPro; IPR012259; DHFR.
DR   InterPro; IPR024072; DHFR-like_dom.
DR   InterPro; IPR017925; DHFR_CS.
DR   InterPro; IPR001796; DHFR_dom.
DR   Pfam; PF00186; DHFR_1; 1.
DR   PIRSF; PIRSF000194; DHFR; 1.
DR   PRINTS; PR00070; DHFR.
DR   SUPFAM; SSF53597; SSF53597; 1.
DR   PROSITE; PS00075; DHFR_1; 1.
DR   PROSITE; PS51330; DHFR_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; C5B7N8.
DR   SWISS-2DPAGE; C5B7N8.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001485};
KW   NADP {ECO:0000256|PIRNR:PIRNR000194};
KW   One-carbon metabolism {ECO:0000256|PIRNR:PIRNR000194};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR000194,
KW   ECO:0000313|EMBL:ACR67915.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001485}.
FT   DOMAIN        2    159       DHFR (dihydrofolate reductase).
FT                                {ECO:0000259|PROSITE:PS51330}.
SQ   SEQUENCE   160 AA;  17791 MW;  B3CD7847BA6A669B CRC64;
     MIISLIAALS ADRVIGLDNA MPWHLSADLA WFKRNTLAKP VIMGRKTYES IGRPLPGRHN
     IVISRQAGCD DRVSWVATPE AALTAAADAD EVMIIGGGTL YEQFLPQASR LYLTHVDADV
     AGDTYFPDYA PDEWQTCFSE FHEADSDNSH AYCFEILDRR
//

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