(data stored in ACNUC7421 zone)

SWISSPROT: C6AZ61_RHILS

ID   C6AZ61_RHILS            Unreviewed;       272 AA.
AC   C6AZ61;
DT   01-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   01-SEP-2009, sequence version 1.
DT   07-JUN-2017, entry version 60.
DE   RecName: Full=tRNA pseudouridine synthase A {ECO:0000256|HAMAP-Rule:MF_00171};
DE            EC=5.4.99.12 {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridylate synthase I {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA-uridine isomerase I {ECO:0000256|HAMAP-Rule:MF_00171};
GN   Name=truA {ECO:0000256|HAMAP-Rule:MF_00171};
GN   OrderedLocusNames=Rleg_0076 {ECO:0000313|EMBL:ACS54387.1};
OS   Rhizobium leguminosarum bv. trifolii (strain WSM1325).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=395491 {ECO:0000313|EMBL:ACS54387.1, ECO:0000313|Proteomes:UP000002256};
RN   [1] {ECO:0000313|EMBL:ACS54387.1, ECO:0000313|Proteomes:UP000002256}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WSM1325 {ECO:0000313|EMBL:ACS54387.1,
RC   ECO:0000313|Proteomes:UP000002256};
RX   PubMed=21304718;
RA   Reeve W., O'Hara G., Chain P., Ardley J., Brau L., Nandesena K.,
RA   Tiwari R., Copeland A., Nolan M., Han C., Brettin T., Land M.,
RA   Ovchinikova G., Ivanova N., Mavromatis K., Markowitz V., Kyrpides N.,
RA   Melino V., Denton M., Yates R., Howieson J.;
RT   "Complete genome sequence of Rhizobium leguminosarum bv. trifolii
RT   strain WSM1325, an effective microsymbiont of annual Mediterranean
RT   clovers.";
RL   Stand. Genomic Sci. 2:347-356(2010).
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs. {ECO:0000256|HAMAP-
CC       Rule:MF_00171, ECO:0000256|SAAS:SAAS00636255}.
CC   -!- CATALYTIC ACTIVITY: tRNA uridine(38-40) = tRNA pseudouridine(38-
CC       40). {ECO:0000256|HAMAP-Rule:MF_00171,
CC       ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00636268}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00171}.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC       family. {ECO:0000256|HAMAP-Rule:MF_00171,
CC       ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00636204}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00171}.
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DR   EMBL; CP001622; ACS54387.1; -; Genomic_DNA.
DR   RefSeq; WP_012755817.1; NC_012850.1.
DR   ProteinModelPortal; C6AZ61; -.
DR   EnsemblBacteria; ACS54387; ACS54387; Rleg_0076.
DR   KEGG; rlg:Rleg_0076; -.
DR   HOGENOM; HOG000248672; -.
DR   KO; K06173; -.
DR   OMA; FLYGMVR; -.
DR   OrthoDB; POG091H010Q; -.
DR   Proteomes; UP000002256; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
DR   PRODOM; C6AZ61.
DR   SWISS-2DPAGE; C6AZ61.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002256};
KW   Isomerase {ECO:0000256|HAMAP-Rule:MF_00171,
KW   ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00636200,
KW   ECO:0000313|EMBL:ACS54387.1};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00171,
KW   ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00636158}.
FT   DOMAIN        8    106       PseudoU_synth_1. {ECO:0000259|Pfam:
FT                                PF01416}.
FT   DOMAIN      146    248       PseudoU_synth_1. {ECO:0000259|Pfam:
FT                                PF01416}.
FT   ACT_SITE     52     52       Nucleophile. {ECO:0000256|HAMAP-Rule:
FT                                MF_00171, ECO:0000256|PIRSR:PIRSR001430-
FT                                1}.
FT   BINDING     113    113       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_00171, ECO:0000256|PIRSR:PIRSR001430-
FT                                2}.
SQ   SEQUENCE   272 AA;  30191 MW;  5802C7963A124760 CRC64;
     MPRFRMTVEY DGGPYVGWQR QENGHSVQGA IEAAVLSLTG ETVLVRGAGR TDSGVHAMGQ
     VIHADLSKEW SPYQLQNALN AHLRLAGERV SILEVEAAPE FFDARFSALR RHYLYRIVSR
     RAPLALEAGK AWWVPKVLDH EVMHAAAQRL VGKHDFSTFR AAHCQANSPV RTLDRLDVTR
     NGELIEIRAT AQSFLHNQIR SFAGTLKLAG EGKWTPDDVE AALEARDRKA CGPVAPPDGL
     YFMQVDYPDV IPDRRRPVTD TDANDGDADD PS
//

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