(data stored in ACNUC7421 zone)

SWISSPROT: C6AZC3_RHILS

ID   C6AZC3_RHILS            Unreviewed;       229 AA.
AC   C6AZC3;
DT   01-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   01-SEP-2009, sequence version 1.
DT   07-JUN-2017, entry version 63.
DE   RecName: Full=Orotate phosphoribosyltransferase {ECO:0000256|HAMAP-Rule:MF_01208, ECO:0000256|SAAS:SAAS00634338};
DE            Short=OPRT {ECO:0000256|HAMAP-Rule:MF_01208};
DE            Short=OPRTase {ECO:0000256|HAMAP-Rule:MF_01208};
DE            EC=2.4.2.10 {ECO:0000256|HAMAP-Rule:MF_01208, ECO:0000256|SAAS:SAAS00805816};
GN   Name=pyrE {ECO:0000256|HAMAP-Rule:MF_01208};
GN   OrderedLocusNames=Rleg_0138 {ECO:0000313|EMBL:ACS54449.1};
OS   Rhizobium leguminosarum bv. trifolii (strain WSM1325).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=395491 {ECO:0000313|EMBL:ACS54449.1, ECO:0000313|Proteomes:UP000002256};
RN   [1] {ECO:0000313|EMBL:ACS54449.1, ECO:0000313|Proteomes:UP000002256}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WSM1325 {ECO:0000313|EMBL:ACS54449.1,
RC   ECO:0000313|Proteomes:UP000002256};
RX   PubMed=21304718;
RA   Reeve W., O'Hara G., Chain P., Ardley J., Brau L., Nandesena K.,
RA   Tiwari R., Copeland A., Nolan M., Han C., Brettin T., Land M.,
RA   Ovchinikova G., Ivanova N., Mavromatis K., Markowitz V., Kyrpides N.,
RA   Melino V., Denton M., Yates R., Howieson J.;
RT   "Complete genome sequence of Rhizobium leguminosarum bv. trifolii
RT   strain WSM1325, an effective microsymbiont of annual Mediterranean
RT   clovers.";
RL   Stand. Genomic Sci. 2:347-356(2010).
CC   -!- FUNCTION: Catalyzes the transfer of a ribosyl phosphate group from
CC       5-phosphoribose 1-diphosphate to orotate, leading to the formation
CC       of orotidine monophosphate (OMP). {ECO:0000256|HAMAP-
CC       Rule:MF_01208, ECO:0000256|SAAS:SAAS00682396}.
CC   -!- CATALYTIC ACTIVITY: Orotidine 5'-phosphate + diphosphate = orotate
CC       + 5-phospho-alpha-D-ribose 1-diphosphate. {ECO:0000256|HAMAP-
CC       Rule:MF_01208, ECO:0000256|SAAS:SAAS00805810}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01208};
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; UMP from orotate: step 1/2. {ECO:0000256|HAMAP-
CC       Rule:MF_01208, ECO:0000256|SAAS:SAAS00805820}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01208,
CC       ECO:0000256|SAAS:SAAS00805815}.
CC   -!- SIMILARITY: Belongs to the purine/pyrimidine
CC       phosphoribosyltransferase family. PyrE subfamily.
CC       {ECO:0000256|HAMAP-Rule:MF_01208, ECO:0000256|SAAS:SAAS00805822}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_01208}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP001622; ACS54449.1; -; Genomic_DNA.
DR   RefSeq; WP_012755871.1; NC_012850.1.
DR   ProteinModelPortal; C6AZC3; -.
DR   EnsemblBacteria; ACS54449; ACS54449; Rleg_0138.
DR   KEGG; rlg:Rleg_0138; -.
DR   HOGENOM; HOG000037975; -.
DR   KO; K00762; -.
DR   OMA; HAAWVSE; -.
DR   OrthoDB; POG091H034Q; -.
DR   UniPathway; UPA00070; UER00119.
DR   Proteomes; UP000002256; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004588; F:orotate phosphoribosyltransferase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd06223; PRTases_typeI; 1.
DR   HAMAP; MF_01208; PyrE; 1.
DR   InterPro; IPR023031; OPRT.
DR   InterPro; IPR004467; Or_phspho_trans_dom.
DR   InterPro; IPR000836; PRibTrfase_dom.
DR   InterPro; IPR029057; PRTase-like.
DR   Pfam; PF00156; Pribosyltran; 1.
DR   SUPFAM; SSF53271; SSF53271; 1.
DR   TIGRFAMs; TIGR00336; pyrE; 1.
PE   3: Inferred from homology;
DR   PRODOM; C6AZC3.
DR   SWISS-2DPAGE; C6AZC3.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002256};
KW   Glycosyltransferase {ECO:0000256|HAMAP-Rule:MF_01208,
KW   ECO:0000256|SAAS:SAAS00805817, ECO:0000313|EMBL:ACS54449.1};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_01208};
KW   Pyrimidine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01208,
KW   ECO:0000256|SAAS:SAAS00805821};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_01208,
KW   ECO:0000256|SAAS:SAAS00805823, ECO:0000313|EMBL:ACS54449.1}.
FT   DOMAIN       57    154       Pribosyltran. {ECO:0000259|Pfam:PF00156}.
FT   REGION      133    141       5-phosphoribose 1-diphosphate binding.
FT                                {ECO:0000256|HAMAP-Rule:MF_01208}.
FT   BINDING     107    107       5-phosphoribose 1-diphosphate; shared
FT                                with dimeric partner. {ECO:0000256|HAMAP-
FT                                Rule:MF_01208}.
FT   BINDING     108    108       5-phosphoribose 1-diphosphate.
FT                                {ECO:0000256|HAMAP-Rule:MF_01208}.
FT   BINDING     111    111       5-phosphoribose 1-diphosphate; shared
FT                                with dimeric partner. {ECO:0000256|HAMAP-
FT                                Rule:MF_01208}.
FT   BINDING     113    113       5-phosphoribose 1-diphosphate; shared
FT                                with dimeric partner. {ECO:0000256|HAMAP-
FT                                Rule:MF_01208}.
FT   BINDING     137    137       Orotate. {ECO:0000256|HAMAP-Rule:
FT                                MF_01208}.
SQ   SEQUENCE   229 AA;  25092 MW;  F572E02705B25F6B CRC64;
     MIQTTFPDRA VMAELLAKML WEIKAVHFNA AQPYKLSSGM ASPVYIDCRK LLSFPRIRST
     VMDFAASILL RDAGFEQFDC IAGGETAGIP FAALLADRLG LPMIYVRKQP KGHGRNAQIE
     GNMPEGSRVL VIEDLTTAGG SMFKFIDAVR AAGGIVDHGI ALFFYGIFGE QRFADGKVRL
     HHIATWRNVL AVAKEQKLFD DKTLSEVEAF LDAPLAWSGR NGGVSELSL
//

If you have problems or comments...

PBIL Back to PBIL home page