(data stored in ACNUC7421 zone)

SWISSPROT: C6B2K6_RHILS

ID   C6B2K6_RHILS            Unreviewed;       145 AA.
AC   C6B2K6;
DT   01-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   01-SEP-2009, sequence version 1.
DT   07-JUN-2017, entry version 54.
DE   RecName: Full=tRNA-specific adenosine deaminase {ECO:0000256|HAMAP-Rule:MF_00972};
DE            EC=3.5.4.33 {ECO:0000256|HAMAP-Rule:MF_00972};
GN   Name=tadA {ECO:0000256|HAMAP-Rule:MF_00972};
GN   OrderedLocusNames=Rleg_0521 {ECO:0000313|EMBL:ACS54826.1};
OS   Rhizobium leguminosarum bv. trifolii (strain WSM1325).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=395491 {ECO:0000313|EMBL:ACS54826.1, ECO:0000313|Proteomes:UP000002256};
RN   [1] {ECO:0000313|EMBL:ACS54826.1, ECO:0000313|Proteomes:UP000002256}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WSM1325 {ECO:0000313|EMBL:ACS54826.1,
RC   ECO:0000313|Proteomes:UP000002256};
RX   PubMed=21304718;
RA   Reeve W., O'Hara G., Chain P., Ardley J., Brau L., Nandesena K.,
RA   Tiwari R., Copeland A., Nolan M., Han C., Brettin T., Land M.,
RA   Ovchinikova G., Ivanova N., Mavromatis K., Markowitz V., Kyrpides N.,
RA   Melino V., Denton M., Yates R., Howieson J.;
RT   "Complete genome sequence of Rhizobium leguminosarum bv. trifolii
RT   strain WSM1325, an effective microsymbiont of annual Mediterranean
RT   clovers.";
RL   Stand. Genomic Sci. 2:347-356(2010).
CC   -!- FUNCTION: Catalyzes the deamination of adenosine to inosine at the
CC       wobble position 34 of tRNA(Arg2). {ECO:0000256|HAMAP-
CC       Rule:MF_00972}.
CC   -!- CATALYTIC ACTIVITY: Adenine(34) in tRNA + H(2)O = hypoxanthine(34)
CC       in tRNA + NH(3). {ECO:0000256|HAMAP-Rule:MF_00972}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00972};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-
CC       Rule:MF_00972};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00972}.
CC   -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC       family. {ECO:0000256|HAMAP-Rule:MF_00972}.
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DR   EMBL; CP001622; ACS54826.1; -; Genomic_DNA.
DR   RefSeq; WP_012756211.1; NC_012850.1.
DR   ProteinModelPortal; C6B2K6; -.
DR   EnsemblBacteria; ACS54826; ACS54826; Rleg_0521.
DR   KEGG; rlg:Rleg_0521; -.
DR   HOGENOM; HOG000085050; -.
DR   OMA; LEPCQMC; -.
DR   OrthoDB; POG091H01W1; -.
DR   Proteomes; UP000002256; Chromosome.
DR   GO; GO:0052717; F:tRNA-specific adenosine-34 deaminase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0002100; P:tRNA wobble adenosine to inosine editing; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_00972; tRNA_aden_deaminase; 1.
DR   InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd.
DR   InterPro; IPR002125; CMP_dCMP_dom.
DR   InterPro; IPR016193; Cytidine_deaminase-like.
DR   InterPro; IPR028883; tRNA_aden_deaminase.
DR   Pfam; PF14437; MafB19-deam; 1.
DR   SUPFAM; SSF53927; SSF53927; 1.
DR   PROSITE; PS00903; CYT_DCMP_DEAMINASES_1; 1.
DR   PROSITE; PS51747; CYT_DCMP_DEAMINASES_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; C6B2K6.
DR   SWISS-2DPAGE; C6B2K6.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002256};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00972};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00972};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00972};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00972}.
FT   DOMAIN        1    104       CMP/dCMP-type deaminase.
FT                                {ECO:0000259|PROSITE:PS51747}.
FT   ACT_SITE     48     48       Proton donor. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
FT   METAL        46     46       Zinc; via pros nitrogen; catalytic.
FT                                {ECO:0000256|HAMAP-Rule:MF_00972}.
FT   METAL        76     76       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
FT   METAL        79     79       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
SQ   SEQUENCE   145 AA;  15784 MW;  069A17B49B16D6F0 CRC64;
     MEMALEEARA AGERGEVPIG AVVVIDDIAV SRSGNRTREL KDVTAHAEIA AIRLACEALG
     QERLAGADLY VTLEPCTMCA AAISFARIRR LYYGAEDPKG GGVDNGVRFY GQPTCHHAPE
     VYSGFNEVQS ANLLRRFFSQ RREAP
//

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