(data stored in ACNUC7421 zone)

SWISSPROT: RSMH_ECOBD

ID   RSMH_ECOBD              Reviewed;         313 AA.
AC   C5W331; C6EAX3;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-SEP-2009, sequence version 1.
DT   05-JUL-2017, entry version 54.
DE   RecName: Full=Ribosomal RNA small subunit methyltransferase H {ECO:0000255|HAMAP-Rule:MF_01007};
DE            EC=2.1.1.199 {ECO:0000255|HAMAP-Rule:MF_01007};
DE   AltName: Full=16S rRNA m(4)C1402 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01007};
DE   AltName: Full=rRNA (cytosine-N(4)-)-methyltransferase RsmH {ECO:0000255|HAMAP-Rule:MF_01007};
GN   Name=rsmH {ECO:0000255|HAMAP-Rule:MF_01007}; Synonyms=mraW;
GN   OrderedLocusNames=ECBD_3535, ECD_00083, B21_00082;
OS   Escherichia coli (strain B / BL21-DE3).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=469008;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B / BL21-DE3;
RA   Leparc G., Striedner G., Bayer K., Kreil D., Krempl P.M.;
RT   "Sequencing and gene expression analysis of Escherichia coli BL21.";
RL   Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B / BL21-DE3;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R.,
RA   Rubin E.;
RT   "Complete sequence of Escherichia coli BL21(DE3).";
RL   Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B / BL21-DE3;
RX   PubMed=19786035; DOI=10.1016/j.jmb.2009.09.052;
RA   Jeong H., Barbe V., Lee C.H., Vallenet D., Yu D.S., Choi S.H.,
RA   Couloux A., Lee S.W., Yoon S.H., Cattolico L., Hur C.G., Park H.S.,
RA   Segurens B., Kim S.C., Oh T.K., Lenski R.E., Studier F.W.,
RA   Daegelen P., Kim J.F.;
RT   "Genome sequences of Escherichia coli B strains REL606 and
RT   BL21(DE3).";
RL   J. Mol. Biol. 394:644-652(2009).
CC   -!- FUNCTION: Specifically methylates the N4 position of cytidine in
CC       position 1402 (C1402) of 16S rRNA. {ECO:0000255|HAMAP-
CC       Rule:MF_01007}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + cytosine(1402) in
CC       16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402)
CC       in 16S rRNA. {ECO:0000255|HAMAP-Rule:MF_01007}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01007}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmH
CC       family. {ECO:0000255|HAMAP-Rule:MF_01007}.
DR   EMBL; AM946981; CAQ30599.1; -; Genomic_DNA.
DR   EMBL; CP001665; ACT30534.1; -; Genomic_DNA.
DR   EMBL; CP001509; ACT41984.1; -; Genomic_DNA.
DR   RefSeq; WP_000970479.1; NC_012971.2.
DR   ProteinModelPortal; C5W331; -.
DR   SMR; C5W331; -.
DR   EnsemblBacteria; ACT30534; ACT30534; ECBD_3535.
DR   EnsemblBacteria; ACT41984; ACT41984; ECD_00083.
DR   EnsemblBacteria; CAQ30599; CAQ30599; B21_00082.
DR   KEGG; ebd:ECBD_3535; -.
DR   KEGG; ebe:B21_00082; -.
DR   KEGG; ebl:ECD_00083; -.
DR   PATRIC; fig|469008.15.peg.81; -.
DR   eggNOG; ENOG4105CGJ; Bacteria.
DR   eggNOG; COG0275; LUCA.
DR   HOGENOM; HOG000049778; -.
DR   KO; K03438; -.
DR   OMA; KKIHPAT; -.
DR   Proteomes; UP000002032; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.170; -; 1.
DR   HAMAP; MF_01007; 16SrRNA_methyltr_H; 1.
DR   InterPro; IPR002903; RsmH.
DR   InterPro; IPR023397; SAM-dep_MeTrfase_MraW_recog.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   PANTHER; PTHR11265; PTHR11265; 1.
DR   Pfam; PF01795; Methyltransf_5; 1.
DR   PIRSF; PIRSF004486; MraW; 1.
DR   SUPFAM; SSF53335; SSF53335; 2.
DR   SUPFAM; SSF81799; SSF81799; 1.
DR   TIGRFAMs; TIGR00006; TIGR00006; 1.
PE   3: Inferred from homology;
DR   PRODOM; C5W331.
DR   SWISS-2DPAGE; C5W331.
KW   Complete proteome; Cytoplasm; Methyltransferase; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    313       Ribosomal RNA small subunit
FT                                methyltransferase H.
FT                                /FTId=PRO_0000386869.
FT   REGION       35     37       S-adenosyl-L-methionine binding.
FT                                {ECO:0000255|HAMAP-Rule:MF_01007}.
FT   BINDING      55     55       S-adenosyl-L-methionine.
FT                                {ECO:0000255|HAMAP-Rule:MF_01007}.
FT   BINDING      79     79       S-adenosyl-L-methionine; via amide
FT                                nitrogen. {ECO:0000255|HAMAP-
FT                                Rule:MF_01007}.
FT   BINDING     101    101       S-adenosyl-L-methionine.
FT                                {ECO:0000255|HAMAP-Rule:MF_01007}.
FT   BINDING     108    108       S-adenosyl-L-methionine.
FT                                {ECO:0000255|HAMAP-Rule:MF_01007}.
SQ   SEQUENCE   313 AA;  34878 MW;  43174C907A36ECC1 CRC64;
     MMENYKHTTV LLDEAVNGLN IRPDGIYIDG TFGRGGHSRL ILSQLGEEGR LLAIDRDPQA
     IAVAKTIDDP RFSIIHGPFS ALGEYVAERD LIGKIDGILL DLGVSSPQLD DAERGFSFMR
     DGPLDMRMDP TRGQSAAEWL QTAEEADIAW VLKTYGEERF AKRIARAIVE RNREQPMTRT
     KELAEVVAAA TPVKDKFKHP ATRTFQAVRI WVNSELEEIE QALKSSLNVL APGGRLSIIS
     FHSLEDRIVK RFMRENSRGP QVPAGLPMTE EQLKKLGGRQ LRALGKLMPG EEEVAENPRA
     RSSVLRIAER TNA
//

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