(data stored in ACNUC7421 zone)

SWISSPROT: C6WDZ7_ACTMD

ID   C6WDZ7_ACTMD            Unreviewed;       160 AA.
AC   C6WDZ7;
DT   22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   22-SEP-2009, sequence version 1.
DT   07-JUN-2017, entry version 48.
DE   RecName: Full=tRNA-specific adenosine deaminase {ECO:0000256|HAMAP-Rule:MF_00972};
DE            EC=3.5.4.33 {ECO:0000256|HAMAP-Rule:MF_00972};
GN   Name=tadA {ECO:0000256|HAMAP-Rule:MF_00972};
GN   OrderedLocusNames=Amir_0171 {ECO:0000313|EMBL:ACU34142.1};
OS   Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU
OS   3971).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Actinosynnema.
OX   NCBI_TaxID=446462 {ECO:0000313|EMBL:ACU34142.1, ECO:0000313|Proteomes:UP000002213};
RN   [1] {ECO:0000313|EMBL:ACU34142.1, ECO:0000313|Proteomes:UP000002213}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971
RC   {ECO:0000313|Proteomes:UP000002213};
RX   PubMed=21304636; DOI=10.4056/sigs.21137;
RA   Land M., Lapidus A., Mayilraj S., Chen F., Copeland A., Del Rio T.G.,
RA   Nolan M., Lucas S., Tice H., Cheng J.F., Chertkov O., Bruce D.,
RA   Goodwin L., Pitluck S., Rohde M., Goker M., Pati A., Ivanova N.,
RA   Mavromatis K., Chen A., Palaniappan K., Hauser L., Chang Y.J.,
RA   Jeffries C.C., Brettin T., Detter J.C., Han C., Chain P.,
RA   Tindall B.J., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA   Kyrpides N.C., Klenk H.P.;
RT   "Complete genome sequence of Actinosynnema mirum type strain (101).";
RL   Stand. Genomic Sci. 1:46-53(2009).
CC   -!- FUNCTION: Catalyzes the deamination of adenosine to inosine at the
CC       wobble position 34 of tRNA(Arg2). {ECO:0000256|HAMAP-
CC       Rule:MF_00972}.
CC   -!- CATALYTIC ACTIVITY: Adenine(34) in tRNA + H(2)O = hypoxanthine(34)
CC       in tRNA + NH(3). {ECO:0000256|HAMAP-Rule:MF_00972}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00972};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-
CC       Rule:MF_00972};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00972}.
CC   -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC       family. {ECO:0000256|HAMAP-Rule:MF_00972}.
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DR   EMBL; CP001630; ACU34142.1; -; Genomic_DNA.
DR   ProteinModelPortal; C6WDZ7; -.
DR   STRING; 446462.Amir_0171; -.
DR   EnsemblBacteria; ACU34142; ACU34142; Amir_0171.
DR   KEGG; ami:Amir_0171; -.
DR   eggNOG; ENOG4108Z6B; Bacteria.
DR   eggNOG; COG0590; LUCA.
DR   HOGENOM; HOG000085050; -.
DR   OMA; LEPCQMC; -.
DR   OrthoDB; POG091H01W1; -.
DR   Proteomes; UP000002213; Chromosome.
DR   GO; GO:0052717; F:tRNA-specific adenosine-34 deaminase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0002100; P:tRNA wobble adenosine to inosine editing; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_00972; tRNA_aden_deaminase; 1.
DR   InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd.
DR   InterPro; IPR002125; CMP_dCMP_dom.
DR   InterPro; IPR016193; Cytidine_deaminase-like.
DR   InterPro; IPR028883; tRNA_aden_deaminase.
DR   Pfam; PF14437; MafB19-deam; 1.
DR   SUPFAM; SSF53927; SSF53927; 1.
DR   PROSITE; PS00903; CYT_DCMP_DEAMINASES_1; 1.
DR   PROSITE; PS51747; CYT_DCMP_DEAMINASES_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; C6WDZ7.
DR   SWISS-2DPAGE; C6WDZ7.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002213};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00972};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00972};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002213};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00972};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00972}.
FT   DOMAIN        4    115       CMP/dCMP-type deaminase.
FT                                {ECO:0000259|PROSITE:PS51747}.
FT   ACT_SITE     56     56       Proton donor. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
FT   METAL        54     54       Zinc; via pros nitrogen; catalytic.
FT                                {ECO:0000256|HAMAP-Rule:MF_00972}.
FT   METAL        85     85       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
FT   METAL        88     88       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
SQ   SEQUENCE   160 AA;  16609 MW;  CEA301FF6963D37D CRC64;
     MITAPYDAMV GAALEAAAGA GEDIPIGAAV FGPDGAELAR ACNARERLGD PTAHAEVLAL
     RAAAAVFGDG WRLGGCTLAV TVEPCAMCAG ALVLARVDRV VFGAWEPKTG AAGSLWDVVR
     DRRLNHRAEV VGGVREAECA ALMRDFFAGR DTSERWPESG
//

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