(data stored in ACNUC7421 zone)

SWISSPROT: C8W2Y5_DESAS

ID   C8W2Y5_DESAS            Unreviewed;       276 AA.
AC   C8W2Y5;
DT   03-NOV-2009, integrated into UniProtKB/TrEMBL.
DT   03-NOV-2009, sequence version 1.
DT   08-MAY-2019, entry version 72.
DE   RecName: Full=Thymidylate synthase {ECO:0000256|HAMAP-Rule:MF_00008, ECO:0000256|SAAS:SAAS01077528};
DE            Short=TS {ECO:0000256|HAMAP-Rule:MF_00008};
DE            Short=TSase {ECO:0000256|HAMAP-Rule:MF_00008};
DE            EC=2.1.1.45 {ECO:0000256|HAMAP-Rule:MF_00008};
GN   Name=thyA {ECO:0000256|HAMAP-Rule:MF_00008};
GN   OrderedLocusNames=Dtox_0188 {ECO:0000313|EMBL:ACV61141.1};
OS   Desulfotomaculum acetoxidans (strain ATCC 49208 / DSM 771 / VKM
OS   B-1644) (Desulfofarcimen acetoxidans).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Peptococcaceae;
OC   Desulfofarcimen.
OX   NCBI_TaxID=485916 {ECO:0000313|EMBL:ACV61141.1, ECO:0000313|Proteomes:UP000002217};
RN   [1] {ECO:0000313|EMBL:ACV61141.1, ECO:0000313|Proteomes:UP000002217}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49208 / DSM 771 / VKM B-1644
RC   {ECO:0000313|Proteomes:UP000002217};
RX   PubMed=21304664; DOI=10.4056/sigs.39508;
RA   Spring S., Lapidus A., Schroder M., Gleim D., Sims D., Meincke L.,
RA   Glavina Del Rio T., Tice H., Copeland A., Cheng J.F., Lucas S.,
RA   Chen F., Nolan M., Bruce D., Goodwin L., Pitluck S., Ivanova N.,
RA   Mavromatis K., Mikhailova N., Pati A., Chen A., Palaniappan K.,
RA   Land M., Hauser L., Chang Y.J., Jeffries C.D., Chain P., Saunders E.,
RA   Brettin T., Detter J.C., Goker M., Bristow J., Eisen J.A.,
RA   Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P., Han C.;
RT   "Complete genome sequence of Desulfotomaculum acetoxidans type strain
RT   (5575).";
RL   Stand. Genomic Sci. 1:242-253(2009).
CC   -!- FUNCTION: Catalyzes the reductive methylation of 2'-deoxyuridine-
CC       5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate
CC       (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as
CC       the methyl donor and reductant in the reaction, yielding
CC       dihydrofolate (DHF) as a by-product. This enzymatic reaction
CC       provides an intracellular de novo source of dTMP, an essential
CC       precursor for DNA biosynthesis. {ECO:0000256|HAMAP-Rule:MF_00008}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP =
CC         7,8-dihydrofolate + dTMP; Xref=Rhea:RHEA:12104,
CC         ChEBI:CHEBI:15636, ChEBI:CHEBI:57451, ChEBI:CHEBI:63528,
CC         ChEBI:CHEBI:246422; EC=2.1.1.45; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00008, ECO:0000256|SAAS:SAAS01115414};
CC   -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis.
CC       {ECO:0000256|HAMAP-Rule:MF_00008, ECO:0000256|SAAS:SAAS01082420}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00008,
CC       ECO:0000256|SAAS:SAAS01077531}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00008}.
CC   -!- SIMILARITY: Belongs to the thymidylate synthase family. Bacterial-
CC       type ThyA subfamily. {ECO:0000256|HAMAP-Rule:MF_00008}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00008}.
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DR   EMBL; CP001720; ACV61141.1; -; Genomic_DNA.
DR   RefSeq; WP_015755862.1; NC_013216.1.
DR   STRING; 485916.Dtox_0188; -.
DR   EnsemblBacteria; ACV61141; ACV61141; Dtox_0188.
DR   KEGG; dae:Dtox_0188; -.
DR   eggNOG; ENOG4105C0V; Bacteria.
DR   eggNOG; COG0207; LUCA.
DR   HOGENOM; HOG000257898; -.
DR   KO; K00560; -.
DR   OMA; WNEWEVG; -.
DR   OrthoDB; 1212177at2; -.
DR   BioCyc; DACE485916:G1GFV-194-MONOMER; -.
DR   UniPathway; UPA00575; -.
DR   Proteomes; UP000002217; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004799; F:thymidylate synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006231; P:dTMP biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00351; TS_Pyrimidine_HMase; 1.
DR   Gene3D; 3.30.572.10; -; 1.
DR   HAMAP; MF_00008; Thymidy_synth_bact; 1.
DR   InterPro; IPR023451; Thymidate_synth/dCMP_Mease.
DR   InterPro; IPR036926; Thymidate_synth/dCMP_Mease_sf.
DR   InterPro; IPR000398; Thymidylate_synthase.
DR   InterPro; IPR020940; Thymidylate_synthase_AS.
DR   Pfam; PF00303; Thymidylat_synt; 1.
DR   PRINTS; PR00108; THYMDSNTHASE.
DR   SUPFAM; SSF55831; SSF55831; 1.
DR   TIGRFAMs; TIGR03284; thym_sym; 1.
DR   PROSITE; PS00091; THYMIDYLATE_SYNTHASE; 1.
PE   3: Inferred from homology;
DR   PRODOM; C8W2Y5.
DR   SWISS-2DPAGE; C8W2Y5.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002217};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00008};
KW   Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00008,
KW   ECO:0000256|SAAS:SAAS01077523, ECO:0000313|EMBL:ACV61141.1};
KW   Nucleotide biosynthesis {ECO:0000256|HAMAP-Rule:MF_00008,
KW   ECO:0000256|SAAS:SAAS01082423};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002217};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00008,
KW   ECO:0000256|SAAS:SAAS00013237, ECO:0000313|EMBL:ACV61141.1}.
FT   DOMAIN        8    274       Thymidylat_synt. {ECO:0000259|Pfam:
FT                                PF00303}.
FT   NP_BIND     137    138       dUMP; shared with dimeric partner.
FT                                {ECO:0000256|HAMAP-Rule:MF_00008}.
FT   NP_BIND     177    180       dUMP. {ECO:0000256|HAMAP-Rule:MF_00008}.
FT   NP_BIND     218    220       dUMP. {ECO:0000256|HAMAP-Rule:MF_00008}.
FT   ACT_SITE    157    157       {ECO:0000256|PROSITE-ProRule:PRU10016}.
FT   ACT_SITE    157    157       Nucleophile. {ECO:0000256|HAMAP-Rule:
FT                                MF_00008}.
FT   BINDING      64     64       5,10-methylenetetrahydrofolate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00008}.
FT   BINDING     180    180       5,10-methylenetetrahydrofolate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00008}.
FT   BINDING     188    188       dUMP. {ECO:0000256|HAMAP-Rule:MF_00008}.
FT   BINDING     275    275       5,10-methylenetetrahydrofolate; via
FT                                carbonyl oxygen. {ECO:0000256|HAMAP-Rule:
FT                                MF_00008}.
SQ   SEQUENCE   276 AA;  32016 MW;  A9D16DBB3BED7FB4 CRC64;
     MSKADDVFIG MCKEILDKGI SSEGEEVRAK WTDGMPAHTI KRFAVVNRYN LSEEFPILTL
     RPTNLKAAID ELLWIWQKKS NNINDLNSSI WDSWANDEGS IGKAYGYQLG IKHKYREGEF
     DQVDRILYDL RHTPYSRRII ANMYNHSDLH EMNLYPCAYS MTFNVTGRKL NAILNQRSQD
     VLVANNWNVA QYAILIHMFA QVSSFEAGEF VHVIADAHIY DRHIPIIKEL IQRTSYPAPR
     LLINPDKKHF YDFTVDDFLL EGYQTNEQIR NIPVAV
//

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