(data stored in SCRATCH3701 zone)

SWISSPROT: D5E8Q7_METMS

ID   D5E8Q7_METMS            Unreviewed;       273 AA.
AC   D5E8Q7;
DT   15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT   15-JUN-2010, sequence version 1.
DT   11-DEC-2019, entry version 62.
DE   RecName: Full=tRNA pseudouridine synthase A {ECO:0000256|HAMAP-Rule:MF_00171};
DE            EC=5.4.99.12 {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridylate synthase I {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA-uridine isomerase I {ECO:0000256|HAMAP-Rule:MF_00171};
GN   Name=truA {ECO:0000256|HAMAP-Rule:MF_00171};
GN   OrderedLocusNames=Mmah_0028 {ECO:0000313|EMBL:ADE35566.1};
OS   Methanohalophilus mahii (strain ATCC 35705 / DSM 5219 / SLP).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanohalophilus.
OX   NCBI_TaxID=547558 {ECO:0000313|EMBL:ADE35566.1, ECO:0000313|Proteomes:UP000001059};
RN   [1] {ECO:0000313|EMBL:ADE35566.1, ECO:0000313|Proteomes:UP000001059}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35705 / DSM 5219 / SLP
RC   {ECO:0000313|Proteomes:UP000001059};
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N.,
RA   Lykidis A., Saunders E., Brettin T., Detter J.C., Han C., Land M.,
RA   Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D.,
RA   Spring S., Schneider S., Schroeder M., Klenk H.-P., Eisen J.A.;
RT   "The complete genome of Methanohalophilus mahii DSM 5219.";
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in the
CC       anticodon stem and loop of transfer RNAs. {ECO:0000256|HAMAP-
CC       Rule:MF_00171}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC         Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00171,
CC         ECO:0000256|RuleBase:RU003792};
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000256|HAMAP-Rule:MF_00171, ECO:0000256|RuleBase:RU003792}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|HAMAP-Rule:MF_00171}.
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DR   EMBL; CP001994; ADE35566.1; -; Genomic_DNA.
DR   RefSeq; WP_013036509.1; NC_014002.1.
DR   EnsemblBacteria; ADE35566; ADE35566; Mmah_0028.
DR   GeneID; 8982159; -.
DR   KEGG; mmh:Mmah_0028; -.
DR   eggNOG; arCOG04449; Archaea.
DR   eggNOG; COG0101; LUCA.
DR   HOGENOM; HOG000248673; -.
DR   KO; K06173; -.
DR   OMA; FLYGMVR; -.
DR   OrthoDB; 62389at2157; -.
DR   BioCyc; MMAH547558:G1GHT-28-MONOMER; -.
DR   Proteomes; UP000001059; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 1.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
DR   PRODOM; D5E8Q7.
DR   SWISS-2DPAGE; D5E8Q7.
KW   Isomerase {ECO:0000256|HAMAP-Rule:MF_00171, ECO:0000256|RuleBase:RU003792,
KW   ECO:0000313|EMBL:ADE35566.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001059};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00171,
KW   ECO:0000256|RuleBase:RU003792}.
FT   DOMAIN          128..228
FT                   /note="PseudoU_synth_1"
FT                   /evidence="ECO:0000259|Pfam:PF01416"
FT   ACT_SITE        55
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00171,
FT                   ECO:0000256|PIRSR:PIRSR001430-1"
FT   BINDING         111
FT                   /note="Substrate"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00171,
FT                   ECO:0000256|PIRSR:PIRSR001430-2"
SQ   SEQUENCE   273 AA;  30896 MW;  AC454A93D650D34E CRC64;
     MRVALKIAYI GSSYRGSQVQ PGVSTIEGKI FEALQSLEII EDPSSARFVS SGRTDTGVHA
     MGQVVAFDTD KPKLAIPRVI NSKLPGDIWA WSRAEVDSDF HPRRYAINRT YRYITPSMDA
     DISKLRAACS ILEGSHDFAN FCFRDTGRST IRRIEKIDVR LAGNLLRIDI TANSFLWKMI
     RKIVTVLLLV GSGARDLDWL ENMLDPASYE EGIKPAKPYG LILTNVNYGD SVQWVDDGYS
     MRRSRERIEE YLVYHQVLAE VMDQFLPKEP KAE
//

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