(data stored in ACNUC1104 zone)

SWISSPROT: T3HPD_STAND

ID   T3HPD_STAND             Reviewed;         345 AA.
AC   D7A0Y1;
DT   24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 1.
DT   08-MAY-2019, entry version 41.
DE   RecName: Full=Trans-3-hydroxy-L-proline dehydratase {ECO:0000303|PubMed:25608448};
DE            Short=T3LHyp dehydratase {ECO:0000303|PubMed:25608448};
DE            Short=t3HypD {ECO:0000303|PubMed:25608448};
DE            EC=4.2.1.77 {ECO:0000269|PubMed:25608448};
GN   OrderedLocusNames=Snov_0155 {ECO:0000312|EMBL:ADH87491.1};
OS   Starkeya novella (strain ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 /
OS   NBRC 12443 / NCIB 9113).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Xanthobacteraceae; Starkeya.
OX   NCBI_TaxID=639283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB
RC   9113;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Davenport K., Brettin T., Detter J.C., Han C., Tapia R.,
RA   Land M., Hauser L., Kyrpides N., Ivanova N., Beatson S., Kappler U.,
RA   Woyke T.;
RT   "Complete sequence of Starkeya novella DSM 506.";
RL   Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB
RC   9113;
RX   PubMed=25608448; DOI=10.1021/ja5103986;
RA   Zhang X., Kumar R., Vetting M.W., Zhao S., Jacobson M.P., Almo S.C.,
RA   Gerlt J.A.;
RT   "A unique cis-3-hydroxy-L-proline dehydratase in the enolase
RT   superfamily.";
RL   J. Am. Chem. Soc. 137:1388-1391(2015).
CC   -!- FUNCTION: Catalyzes the dehydration of trans-3-hydroxy-L-proline
CC       (t3LHyp) to Delta(1)-pyrroline-2-carboxylate (Pyr2C). May be
CC       involved in a degradation pathway that converts t3LHyp to L-
CC       proline, which would allow S.novella to grow on t3LHyp as a sole
CC       carbon source. {ECO:0000269|PubMed:25608448}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=trans-3-hydroxy-L-proline = 1-pyrroline-2-carboxylate +
CC         H2O; Xref=Rhea:RHEA:10320, ChEBI:CHEBI:15377, ChEBI:CHEBI:39785,
CC         ChEBI:CHEBI:57938; EC=4.2.1.77;
CC         Evidence={ECO:0000269|PubMed:25608448};
CC   -!- SIMILARITY: Belongs to the proline racemase family. {ECO:0000305}.
DR   EMBL; CP002026; ADH87491.1; -; Genomic_DNA.
DR   RefSeq; WP_013164996.1; NC_014217.1.
DR   SMR; D7A0Y1; -.
DR   STRING; 639283.Snov_0155; -.
DR   EnsemblBacteria; ADH87491; ADH87491; Snov_0155.
DR   KEGG; sno:Snov_0155; -.
DR   eggNOG; ENOG4105CTU; Bacteria.
DR   eggNOG; COG3938; LUCA.
DR   HOGENOM; HOG000084336; -.
DR   KO; K22548; -.
DR   OMA; GWIIMEP; -.
DR   OrthoDB; 559014at2; -.
DR   BioCyc; SNOV639283:G1GLM-157-MONOMER; -.
DR   Proteomes; UP000006633; Chromosome.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0050346; F:trans-L-3-hydroxyproline dehydratase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR008794; Pro_racemase_fam.
DR   PANTHER; PTHR33442; PTHR33442; 1.
DR   Pfam; PF05544; Pro_racemase; 1.
DR   PIRSF; PIRSF029792; Pro_racemase; 1.
DR   SFLD; SFLDS00028; Proline_Racemase; 1.
PE   1: Evidence at protein level;
DR   PRODOM; D7A0Y1.
DR   SWISS-2DPAGE; D7A0Y1.
KW   Complete proteome; Isomerase; Lyase; Reference proteome.
FT   CHAIN         1    345       Trans-3-hydroxy-L-proline dehydratase.
FT                                /FTId=PRO_0000433401.
FT   REGION       91     92       Substrate binding.
FT                                {ECO:0000250|UniProtKB:B9K4G4}.
FT   REGION      257    258       Substrate binding.
FT                                {ECO:0000250|UniProtKB:B9K4G4}.
FT   ACT_SITE     90     90       Proton acceptor.
FT                                {ECO:0000250|UniProtKB:B9K4G4}.
FT   BINDING     252    252       Substrate.
FT                                {ECO:0000250|UniProtKB:B9K4G4}.
SQ   SEQUENCE   345 AA;  37028 MW;  9841A83C8563BCFD CRC64;
     MRSSKVIHVV GCHAEGEVGD VIVGGVAPPP GETVWAQSRF VASDNTLRNF VLQEPRGGVF
     RHVNLLVPPK NKEAVAAWII MEPEDTPPMS GSNSICVSTV LLDTGIVPMV EPETHMVLEA
     PGGLIEATAY CKNGKAERIR VKNHPSFADK LDAKLELEGY GTLTVDTAYG GDSFCIVDAH
     ALGFSIKPDE AKDFADLGMK IVKAANQQLG FQHPTNKDWS HISFCQFAAP LTDDNGTPSG
     ANAVAIRPGK IDRSPCGTGC SARMAVLHAK GILKVGDAFV GRSIIGSRFD CRVEAETSIG
     GRPAIVPSIM GRAFITHTAQ LMVDPDDPWQ TGYRLSDTWP VWKQD
//

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