(data stored in ACNUC1104 zone)

SWISSPROT: 4HYPE_STAND

ID   4HYPE_STAND             Reviewed;         333 AA.
AC   D7A0Y3;
DT   24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 1.
DT   08-MAY-2019, entry version 39.
DE   RecName: Full=4-hydroxyproline 2-epimerase {ECO:0000303|PubMed:25608448};
DE            Short=4Hyp 2-epimerase;
DE            Short=4HypE {ECO:0000303|PubMed:25608448};
DE            EC=5.1.1.8 {ECO:0000269|PubMed:25608448};
GN   OrderedLocusNames=Snov_0157 {ECO:0000312|EMBL:ADH87493.1};
OS   Starkeya novella (strain ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 /
OS   NBRC 12443 / NCIB 9113).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Xanthobacteraceae; Starkeya.
OX   NCBI_TaxID=639283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB
RC   9113;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Davenport K., Brettin T., Detter J.C., Han C., Tapia R.,
RA   Land M., Hauser L., Kyrpides N., Ivanova N., Beatson S., Kappler U.,
RA   Woyke T.;
RT   "Complete sequence of Starkeya novella DSM 506.";
RL   Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB
RC   9113;
RX   PubMed=25608448; DOI=10.1021/ja5103986;
RA   Zhang X., Kumar R., Vetting M.W., Zhao S., Jacobson M.P., Almo S.C.,
RA   Gerlt J.A.;
RT   "A unique cis-3-hydroxy-L-proline dehydratase in the enolase
RT   superfamily.";
RL   J. Am. Chem. Soc. 137:1388-1391(2015).
CC   -!- FUNCTION: Catalyzes the epimerization of trans-4-hydroxy-L-proline
CC       (t4LHyp) to cis-4-hydroxy-D-proline (c4DHyp). May be involved in a
CC       degradation pathway of t4LHyp, which would allow S.novella to grow
CC       on t4LHyp as a sole carbon source. {ECO:0000269|PubMed:25608448}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline;
CC         Xref=Rhea:RHEA:21152, ChEBI:CHEBI:57690, ChEBI:CHEBI:58375;
CC         EC=5.1.1.8; Evidence={ECO:0000269|PubMed:25608448};
CC   -!- SIMILARITY: Belongs to the proline racemase family. {ECO:0000305}.
DR   EMBL; CP002026; ADH87493.1; -; Genomic_DNA.
DR   RefSeq; WP_013164998.1; NC_014217.1.
DR   SMR; D7A0Y3; -.
DR   STRING; 639283.Snov_0157; -.
DR   EnsemblBacteria; ADH87493; ADH87493; Snov_0157.
DR   KEGG; sno:Snov_0157; -.
DR   eggNOG; ENOG4105CTU; Bacteria.
DR   eggNOG; COG3938; LUCA.
DR   HOGENOM; HOG000084336; -.
DR   KO; K12658; -.
DR   OMA; CPCGLVT; -.
DR   OrthoDB; 559014at2; -.
DR   BioCyc; SNOV639283:G1GLM-159-MONOMER; -.
DR   Proteomes; UP000006633; Chromosome.
DR   GO; GO:0047580; F:4-hydroxyproline epimerase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR008794; Pro_racemase_fam.
DR   PANTHER; PTHR33442; PTHR33442; 1.
DR   Pfam; PF05544; Pro_racemase; 1.
DR   PIRSF; PIRSF029792; Pro_racemase; 1.
DR   SFLD; SFLDS00028; Proline_Racemase; 1.
PE   1: Evidence at protein level;
DR   PRODOM; D7A0Y3.
DR   SWISS-2DPAGE; D7A0Y3.
KW   Complete proteome; Isomerase; Reference proteome.
FT   CHAIN         1    333       4-hydroxyproline 2-epimerase.
FT                                /FTId=PRO_0000433402.
FT   REGION       91     92       Substrate binding.
FT                                {ECO:0000250|UniProtKB:Q4KGU2}.
FT   REGION      254    255       Substrate binding.
FT                                {ECO:0000250|UniProtKB:Q4KGU2}.
FT   ACT_SITE     90     90       Proton acceptor.
FT                                {ECO:0000250|UniProtKB:Q4KGU2}.
FT   ACT_SITE    253    253       Proton donor.
FT                                {ECO:0000250|UniProtKB:Q4KGU2}.
FT   BINDING     223    223       Substrate.
FT                                {ECO:0000250|UniProtKB:Q4KGU2}.
FT   BINDING     249    249       Substrate.
FT                                {ECO:0000250|UniProtKB:Q4KGU2}.
SQ   SEQUENCE   333 AA;  36144 MW;  64A2E0FBE32FD42D CRC64;
     MARHSFFCID GHTCGNPVRL VAGGGPNLQG ANMIEKRAHF LAEYDWIRTG LMFEPRGHDM
     MSGSILYPPT RPDCDVAILF IETSGCLPMC GHGTIGTVTM AIEHGLVTPK IPGVLMLDTP
     AGVVKAEYRQ EGQYVEEVRI TNVPAFLYAR GLTAECPGLG EVMVDVAYGG NFYAIVEPQE
     HFRDMADFTA GELIGMSGAL RKALNAKYEF VHPEKPEIRG LSHILWTGAP KHAEAHARNA
     VFYGDKAIDR SPCGTGTSAR IAHWAANGKL KVGDDFVHES IIGSLFKGRV EATARVGNVD
     AIIPSIGGWA RMTGYNTIFI DDRDPFAHGF VVV
//

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