(data stored in ACNUC1104 zone)

SWISSPROT: D7A258_STAND

ID   D7A258_STAND            Unreviewed;       456 AA.
AC   D7A258;
DT   10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT   10-AUG-2010, sequence version 1.
DT   08-MAY-2019, entry version 43.
DE   RecName: Full=Glutamate--cysteine ligase {ECO:0000256|PIRNR:PIRNR017901};
DE            EC=6.3.2.2 {ECO:0000256|PIRNR:PIRNR017901};
GN   OrderedLocusNames=Snov_0341 {ECO:0000313|EMBL:ADH87674.1};
OS   Starkeya novella (strain ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 /
OS   NBRC 12443 / NCIB 9113).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Xanthobacteraceae; Starkeya.
OX   NCBI_TaxID=639283 {ECO:0000313|EMBL:ADH87674.1, ECO:0000313|Proteomes:UP000006633};
RN   [1] {ECO:0000313|Proteomes:UP000006633}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB
RC   9113 {ECO:0000313|Proteomes:UP000006633};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Davenport K., Brettin T., Detter J.C., Han C., Tapia R.,
RA   Land M., Hauser L., Kyrpides N., Ivanova N., Beatson S., Kappler U.,
RA   Woyke T.;
RT   "Complete sequence of Starkeya novella DSM 506.";
RL   Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADH87674.1, ECO:0000313|Proteomes:UP000006633}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB
RC   9113 {ECO:0000313|Proteomes:UP000006633};
RX   PubMed=23450099; DOI=10.4056/sigs.3006378;
RA   Kappler U., Davenport K., Beatson S., Lucas S., Lapidus A.,
RA   Copeland A., Berry K.W., Glavina Del Rio T., Hammon N., Dalin E.,
RA   Tice H., Pitluck S., Richardson P., Bruce D., Goodwin L.A., Han C.,
RA   Tapia R., Detter J.C., Chang Y.J., Jeffries C.D., Land M., Hauser L.,
RA   Kyrpides N.C., Goker M., Ivanova N., Klenk H.P., Woyke T.;
RT   "Complete genome sequence of the facultatively chemolithoautotrophic
RT   and methylotrophic alpha Proteobacterium Starkeya novella type strain
RT   (ATCC 8093(T)).";
RL   Stand. Genomic Sci. 7:44-58(2012).
CC   -!- FUNCTION: Catalyzes the synthesis of gamma-glutamylcysteine
CC       (gamma-GC). {ECO:0000256|PIRNR:PIRNR017901}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-cysteine + L-glutamate = ADP + H(+) + L-gamma-
CC         glutamyl-L-cysteine + phosphate; Xref=Rhea:RHEA:13285,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:35235, ChEBI:CHEBI:43474, ChEBI:CHEBI:58173,
CC         ChEBI:CHEBI:456216; EC=6.3.2.2;
CC         Evidence={ECO:0000256|PIRNR:PIRNR017901,
CC         ECO:0000256|SAAS:SAAS01123684};
CC   -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 2
CC       family. EgtA subfamily. {ECO:0000256|PIRNR:PIRNR017901}.
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DR   EMBL; CP002026; ADH87674.1; -; Genomic_DNA.
DR   RefSeq; WP_013165179.1; NC_014217.1.
DR   STRING; 639283.Snov_0341; -.
DR   EnsemblBacteria; ADH87674; ADH87674; Snov_0341.
DR   KEGG; sno:Snov_0341; -.
DR   eggNOG; ENOG4105D1K; Bacteria.
DR   eggNOG; COG3572; LUCA.
DR   HOGENOM; HOG000009857; -.
DR   KO; K01919; -.
DR   OMA; DYVEWAL; -.
DR   OrthoDB; 991285at2; -.
DR   BioCyc; SNOV639283:G1GLM-345-MONOMER; -.
DR   Proteomes; UP000006633; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006750; P:glutathione biosynthetic process; IEA:InterPro.
DR   InterPro; IPR035434; GCL_bact_plant.
DR   InterPro; IPR006336; GCS2.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR011556; Glut_cys_lig_pln_type.
DR   PANTHER; PTHR34378; PTHR34378; 1.
DR   PANTHER; PTHR34378:SF1; PTHR34378:SF1; 1.
DR   Pfam; PF04107; GCS2; 1.
DR   PIRSF; PIRSF017901; GCL; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   TIGRFAMs; TIGR01436; glu_cys_lig_pln; 1.
PE   3: Inferred from homology;
DR   PRODOM; D7A258.
DR   SWISS-2DPAGE; D7A258.
KW   ATP-binding {ECO:0000256|PIRNR:PIRNR017901,
KW   ECO:0000256|SAAS:SAAS00916638};
KW   Complete proteome {ECO:0000313|Proteomes:UP000006633};
KW   Ligase {ECO:0000256|PIRNR:PIRNR017901, ECO:0000256|SAAS:SAAS00916648,
KW   ECO:0000313|EMBL:ADH87674.1};
KW   Nucleotide-binding {ECO:0000256|PIRNR:PIRNR017901,
KW   ECO:0000256|SAAS:SAAS00916623};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006633}.
SQ   SEQUENCE   456 AA;  50654 MW;  43131A8F52885C9A CRC64;
     MARDQIDTQP IESRDDLVAW LEAGCKAPMK FRIGTEHEKF PFTLNGHEPV PYEGPNGIRA
     LLEGMQALNC WEPIMEGSTI IGLADNVGGG AISLEPGGQF ELSGAPLITI HETCAEVNSH
     LTQVREVATP LGFGFLGLGM SPKWTLAQTP VMPKGRYKIM TAYMPKVGTH GLDMMYRTCT
     VQVNLDFSSE ADMVQKLRVG LALQPVATAL FANSPFTEGK PNGFLSFRSE IWRDTDNNRS
     GMLPFAFEKG MGFERYVDYA LDVPMYFIKR GDHYIDVAGS SFRDLLAGKH PAVPGETATV
     SDWANHLSTI FPEVRLKRYL EMRGADGGPW ANLCALPALW TGLLYDQASL DAAWELAKGW
     SADERQKLRD DVPRLGFKAE IAGRSMHELA REVVAISRAG LARRDKRDSQ GRDETRFLQP
     LEESLVSGLT PAEVLLKRYE TEWNRSVEPI FDDCAY
//

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