(data stored in SCRATCH3701 zone)

SWISSPROT: SFRB_GEOSK

ID   SFRB_GEOSK              Reviewed;         672 AA.
AC   D7AF64;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 1.
DT   11-DEC-2019, entry version 45.
DE   RecName: Full=NADPH-Fe(3+) oxidoreductase subunit beta;
DE            EC=1.-.-.-;
DE   AltName: Full=Soluble Fe(3+) reductase beta subunit;
GN   Name=sfrB; OrderedLocusNames=KN400_0499;
OS   Geobacter sulfurreducens (strain DL-1 / KN400).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC   Geobacteraceae; Geobacter.
OX   NCBI_TaxID=663917;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DL-1 / KN400;
RX   PubMed=20544019; DOI=10.1371/journal.pone.0010922;
RA   Nagarajan H., Butler J.E., Klimes A., Qiu Y., Zengler K., Ward J.,
RA   Young N.D., Methe B.A., Palsson B.O., Lovley D.R., Barrett C.L.;
RT   "De Novo assembly of the complete genome of an enhanced electricity-
RT   producing variant of Geobacter sulfurreducens using only short reads.";
RL   PLoS ONE 5:E10922-E10922(2010).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=DL-1 / KN400;
RX   PubMed=17906154; DOI=10.1099/mic.0.2007/006478-0;
RA   Coppi M.V., O'neil R.A., Leang C., Kaufmann F., Methe B.A., Nevin K.P.,
RA   Woodard T.L., Liu A., Lovley D.R.;
RT   "Involvement of Geobacter sulfurreducens SfrAB in acetate metabolism rather
RT   than intracellular, respiration-linked Fe(III) citrate reduction.";
RL   Microbiology 153:3572-3585(2007).
CC   -!- FUNCTION: Probably involved in acetate metabolism and not in the
CC       reduction of Fe(3+) chelates. May serve as a major route for NADP
CC       regeneration. {ECO:0000269|PubMed:17906154}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Heterotetramer with 2 alpha subunits. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:17906154};
CC       Peripheral membrane protein {ECO:0000269|PubMed:17906154}.
CC   -!- DISRUPTION PHENOTYPE: SfrAB-null strain is unable to grow in the
CC       presence of acetate and fumarate unless an additional electron donor is
CC       present. Strongly reduced NADPH-dependent benzyl viologen reductase
CC       activity. {ECO:0000269|PubMed:17906154}.
DR   EMBL; CP002031; ADI83362.1; -; Genomic_DNA.
DR   RefSeq; WP_010941178.1; NC_017454.1.
DR   SMR; D7AF64; -.
DR   PRIDE; D7AF64; -.
DR   EnsemblBacteria; ADI83362; ADI83362; KN400_0499.
DR   KEGG; gsk:KN400_0499; -.
DR   PATRIC; fig|663917.3.peg.506; -.
DR   HOGENOM; HOG000031439; -.
DR   KO; K15022; -.
DR   OMA; QETQCEQ; -.
DR   OrthoDB; 323168at2; -.
DR   BioCyc; GSUL663917:G1GWJ-506-MONOMER; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1060.10; -; 1.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR028261; DPD_II.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR009051; Helical_ferredxn.
DR   InterPro; IPR019575; Nuop51_4Fe4S-bd.
DR   InterPro; IPR037207; Nuop51_4Fe4S-bd_sf.
DR   Pfam; PF14691; Fer4_20; 1.
DR   Pfam; PF10589; NADH_4Fe-4S; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   SMART; SM00928; NADH_4Fe-4S; 1.
DR   SUPFAM; SSF140490; SSF140490; 1.
DR   SUPFAM; SSF46548; SSF46548; 1.
PE   3: Inferred from homology;
DR   PRODOM; D7AF64.
DR   SWISS-2DPAGE; D7AF64.
KW   4Fe-4S; Cell membrane; FAD; Flavoprotein; Iron; Iron-sulfur; Membrane;
KW   Metal-binding; NADP; Oxidoreductase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..672
FT                   /note="NADPH-Fe(3+) oxidoreductase subunit beta"
FT                   /id="PRO_0000429036"
FT   NP_BIND         254..283
FT                   /note="FAD"
FT                   /evidence="ECO:0000255"
FT   NP_BIND         388..421
FT                   /note="NADP"
FT                   /evidence="ECO:0000255"
FT   NP_BIND         552..562
FT                   /note="FAD"
FT                   /evidence="ECO:0000255"
FT   METAL           203
FT                   /note="Iron-sulfur (4Fe-4S)"
FT                   /evidence="ECO:0000255"
FT   METAL           207
FT                   /note="Iron-sulfur (4Fe-4S)"
FT                   /evidence="ECO:0000255"
FT   METAL           211
FT                   /note="Iron-sulfur (4Fe-4S)"
FT                   /evidence="ECO:0000255"
FT   METAL           215
FT                   /note="Iron-sulfur (4Fe-4S)"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   672 AA;  74273 MW;  3779DF2F9AE0D446 CRC64;
     MAQVVFSSWG RTIVDNRKGG EAQDVSFRLP TTLDGERQIA AFMGWDGIIL YDLKVDVPAM
     AAEYMKRVQT QYCCGKCTPG KKGTKVLADV LAAIIEGRAT EADLDTIDDL ADLLTNCKCT
     LCQSSTIPVL DAVKHFREDF LAYITGIRKP ANVHRFIDKY TAPCMDRCPA HIDIPAYIEA
     IKEYRFDESL DIIRDNMPLP SVCGRVCPHP CETHCRRKNV DDSVNIMVLK RSASDYEWMH
     NAAPPMQPKP QKNKKVAIVG AGPAGLACAY YLALEGYPCT IYEALPEGYG GGMIAVGIPP
     YRQPRHLLQR DIDIISSMGV DIIYDTRIGK DISLEELKQK FDAVFLAPGA HRSKPMGVEG
     EDKGYKGFLK GGIDFLREAY MGRPTGMGKK VVVVGGGNTA IDCVRVALRE GAEESTLLYR
     RSRKEMPADV WEVDGADEEG VRFEFQVLPT RVLVDENEQV TGVECVRMAL GEPDASGRRR
     PEPVPGSEFV VECDTVIPAI GQDPDLSFIP DNLGIDITKW NTVVTKYVPL KDAAGKDLKD
     GMGNPLARVL ITDLEGVFAG GDAEIGPLTV VACIGNAHRA ARVIQRWLEE GKAYLTEDEL
     MEDILTNMPV YDKNEKVPWL DSRERAHQAE VHGQERASKG NYQEVELGFV DTQAVEEAER
     CLRCYRVAMA AI
//

If you have problems or comments...

PBIL Back to PBIL home page