(data stored in SCRATCH zone)

SWISSPROT: A0A0F6BHJ2_GEOS0

ID   A0A0F6BHJ2_GEOS0        Unreviewed;       190 AA.
AC   A0A0F6BHJ2;
DT   24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT   24-JUN-2015, sequence version 1.
DT   11-DEC-2019, entry version 22.
DE   RecName: Full=Ribonuclease M5 {ECO:0000256|HAMAP-Rule:MF_01469};
DE            EC=3.1.26.8 {ECO:0000256|HAMAP-Rule:MF_01469};
DE   AltName: Full=RNase M5 {ECO:0000256|HAMAP-Rule:MF_01469};
DE   AltName: Full=Ribosomal RNA terminal maturase M5 {ECO:0000256|HAMAP-Rule:MF_01469};
GN   Name=rnmV {ECO:0000256|HAMAP-Rule:MF_01469};
GN   OrderedLocusNames=GY4MC1_0037 {ECO:0000313|EMBL:ADP72893.1};
OS   Geobacillus sp. (strain Y4.1MC1).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC   unclassified Geobacillus.
OX   NCBI_TaxID=581103 {ECO:0000313|EMBL:ADP72893.1};
RN   [1] {ECO:0000313|EMBL:ADP72893.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y4.1MC1 {ECO:0000313|EMBL:ADP72893.1};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Chertkov O., Zhang X., Detter J.C., Han C., Tapia R., Land M.,
RA   Hauser L., Jeffries C., Kyrpides N., Ivanova N., Ovchinnikova G., Brumm P.,
RA   Mead D., Woyke T.;
RT   "Complete sequence of chromosome of Geobacillus sp. Y4.1MC1.";
RL   Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for correct processing of both the 5' and 3' ends of
CC       5S rRNA precursor. Cleaves both sides of a double-stranded region
CC       yielding mature 5S rRNA in one step. {ECO:0000256|HAMAP-Rule:MF_01469}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of RNA, removing 21 and 42
CC         nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA
CC         precursor.; EC=3.1.26.8; Evidence={ECO:0000256|HAMAP-Rule:MF_01469};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01469}.
CC   -!- SIMILARITY: Belongs to the ribonuclease M5 family. {ECO:0000256|HAMAP-
CC       Rule:MF_01469}.
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DR   EMBL; CP002293; ADP72893.1; -; Genomic_DNA.
DR   EnsemblBacteria; ADP72893; ADP72893; GY4MC1_0037.
DR   KEGG; gmc:GY4MC1_0037; -.
DR   KO; K05985; -.
DR   OMA; RLQMFQI; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0043822; F:ribonuclease M5 activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-UniRule.
DR   CDD; cd01027; TOPRIM_RNase_M5_like; 1.
DR   HAMAP; MF_01469; RNase_M5; 1.
DR   InterPro; IPR004466; RNase_M5.
DR   InterPro; IPR025156; RNase_M5_C.
DR   InterPro; IPR006171; TOPRIM_domain.
DR   InterPro; IPR034141; TOPRIM_RNase_M5-like.
DR   Pfam; PF13331; DUF4093; 1.
DR   Pfam; PF13662; Toprim_4; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   TIGRFAMs; TIGR00334; 5S_RNA_mat_M5; 1.
DR   PROSITE; PS50880; TOPRIM; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0A0F6BHJ2.
DR   SWISS-2DPAGE; A0A0F6BHJ2.
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01469};
KW   Endonuclease {ECO:0000256|HAMAP-Rule:MF_01469};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_01469};
KW   Isomerase {ECO:0000313|EMBL:ADP72893.1};
KW   Nuclease {ECO:0000256|HAMAP-Rule:MF_01469};
KW   Ribosome biogenesis {ECO:0000256|HAMAP-Rule:MF_01469};
KW   RNA-binding {ECO:0000256|HAMAP-Rule:MF_01469};
KW   rRNA processing {ECO:0000256|HAMAP-Rule:MF_01469};
KW   rRNA-binding {ECO:0000256|HAMAP-Rule:MF_01469}.
FT   DOMAIN          5..88
FT                   /note="Toprim"
FT                   /evidence="ECO:0000259|PROSITE:PS50880"
FT   COILED          101..121
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   190 AA;  21354 MW;  DFE079F2FF41DBD9 CRC64;
     MMKIKEIIVV EGKDDTVAIQ RAVDADTIET NGSAISEEII EQIKLAKEKR GVIIFTDPDF
     PGEKIRKTIT ERVPGCKHAF LPRKAAIAKN GKGIGVEHAS VEDIRQALKN VYEETAEWNE
     EITFDELVAA GLIGGQLAKK RRQHLGEILK IGYTNGKQLY KRLQIFQISK EAFYAALEQV
     LQEEANDEYK
//

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