(data stored in SCRATCH zone)

SWISSPROT: A0A0F6BHK6_GEOS0

ID   A0A0F6BHK6_GEOS0        Unreviewed;      1177 AA.
AC   A0A0F6BHK6;
DT   24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT   24-JUN-2015, sequence version 1.
DT   11-DEC-2019, entry version 31.
DE   RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE            Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE            EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN   Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN   OrderedLocusNames=GY4MC1_0051 {ECO:0000313|EMBL:ADP72907.1};
OS   Geobacillus sp. (strain Y4.1MC1).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC   unclassified Geobacillus.
OX   NCBI_TaxID=581103 {ECO:0000313|EMBL:ADP72907.1};
RN   [1] {ECO:0000313|EMBL:ADP72907.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y4.1MC1 {ECO:0000313|EMBL:ADP72907.1};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Chertkov O., Zhang X., Detter J.C., Han C., Tapia R., Land M.,
RA   Hauser L., Jeffries C., Kyrpides N., Ivanova N., Ovchinnikova G., Brumm P.,
RA   Mead D., Woyke T.;
RT   "Complete sequence of chromosome of Geobacillus sp. Y4.1MC1.";
RL   Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC       polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC       release of RNAP and its truncated transcript from the DNA, and
CC       recruitment of nucleotide excision repair machinery to the damaged
CC       site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969,
CC       ECO:0000256|SAAS:SAAS01200955}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC       RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC       {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR   EMBL; CP002293; ADP72907.1; -; Genomic_DNA.
DR   RefSeq; WP_013399809.1; NC_014650.1.
DR   EnsemblBacteria; ADP72907; ADP72907; GY4MC1_0051.
DR   KEGG; gmc:GY4MC1_0051; -.
DR   KO; K03723; -.
DR   OMA; KRGQPGD; -.
DR   OrthoDB; 234717at2; -.
DR   BioCyc; GSP581103:G1GOQ-62-MONOMER; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.30.840; -; 1.
DR   Gene3D; 3.90.1150.50; -; 1.
DR   HAMAP; MF_00969; TRCF; 1.
DR   InterPro; IPR036101; CarD-like/TRCF_dom_sf.
DR   InterPro; IPR003711; CarD-like/TRCF_domain.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR004576; Mfd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR037235; TRCF-like_C.
DR   InterPro; IPR005118; TRCF_C.
DR   InterPro; IPR041471; UvrB_inter.
DR   Pfam; PF02559; CarD_CdnL_TRCF; 1.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF03461; TRCF; 1.
DR   Pfam; PF17757; UvrB_inter; 1.
DR   SMART; SM01058; CarD_TRCF; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00982; TRCF; 1.
DR   SUPFAM; SSF141259; SSF141259; 1.
DR   SUPFAM; SSF143517; SSF143517; 1.
DR   SUPFAM; SSF52540; SSF52540; 4.
DR   TIGRFAMs; TIGR00580; mfd; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0A0F6BHK6.
DR   SWISS-2DPAGE; A0A0F6BHK6.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00969,
KW   ECO:0000256|SAAS:SAAS01200956}; Coiled coil {ECO:0000256|SAM:Coils};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969, ECO:0000256|SAAS:SAAS01200952};
KW   DNA damage {ECO:0000256|HAMAP-Rule:MF_00969,
KW   ECO:0000256|SAAS:SAAS01200947};
KW   DNA repair {ECO:0000256|HAMAP-Rule:MF_00969,
KW   ECO:0000256|SAAS:SAAS01200950};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00969};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00969};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00969,
KW   ECO:0000256|SAAS:SAAS01200961}.
FT   DOMAIN          638..799
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51192"
FT   DOMAIN          824..974
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000259|PROSITE:PS51194"
FT   COILED          308..328
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   COILED          420..440
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   COILED          622..642
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   1177 AA;  135281 MW;  B5D0903DED113256 CRC64;
     MRSLHRYFAE NQDIRSIIGG IEAGLKEQLI VGLSGSARSV FISSLYKETD RPLLVITHNL
     FQAQKIYDDL VQLLGTEEVF LYPVNEVIAT ELAIASPELK AQRLEVMNYW TKRDKGVVIC
     PVAGLRRLLP PVSLWKNNIL TLEVQQNIDV EYCKKQFVQM GYKRVATVSA PGEFSVRGGI
     IDIYPLTAEL PYRIELFDTE IESIRTFTAD DQRSRDEVRQ ITIGPADEMI VYGEMLERGI
     ARIEAGLTES LHKLKDEKAK QLLLEHISSE LEQLKQGQEI EQQYKYMALF YEEPASLLDY
     MPENGILLMD EMSRLQEAAE SLDKEEAEWY TSLLSEGKII HDVPISHSFS QLLQKHRKQR
     IYLSIFLRHV PHTRPENIVN ISCKQMQNFH GQMSLLKSEL ERWKKAKYAV VFLTPNAERM
     KKLQSVLEDY EIDVAPLNRE AVLMHGKYQM IEGDLNTGFE LPMQKLAVIT EEELFKKRVK
     RPIRRQKLSN AERIKSYSEL RVGDYVVHVN HGIGKYLGIE TLEINGVHKD YIHIQYQGSD
     TLYVPVDQID QVQKYVGSEG KEPKIYKLGG SEWKKVKKKV ESSVQDIAED LIKLYAEREA
     SKGYAFSPDT EMQREFEAAF PYQETEDQLR SIEEIKRDME SDKPMDRLLC GDVGYGKTEV
     ALRAAFKAIM DGKQVAFLVP TTILAQQHYE TVRERFQGFP ITVGLLNRFR TRKQQAETIK
     GLKDGTIDMV IGTHRLLSKD VQFKDLGLLI IDEEQRFGVT HKEKIKQLKA NIDVLTLTAT
     PIPRTLHMSM IGVRDLSIIE TPPENRFPVQ TYVMEYTPEL VREAIERELA REGQVFFLYN
     HIEDIDVKAE EISQLVPEAR VTYVHGRMSE TELESTMLAF LEGQYDVLVT TTIIETGVDI
     PNVNTLIVYD ADRMGLSQLY QLRGRVGRSN RVAYAYFTYR KDKVLNEAAE KRLQAIKEFT
     ELGSGFKIAM RDLSIRGAGN ILGAEQHGFI DSVGFDLYSQ MLKEAIEKRK GVKQEEAKHE
     ITIDLEVDAY IPDTYISDGL QKIDMYKRFK AVETMEDVEM LREEMLDRFG EYPDEVAYLF
     QIAEIKVYAK QTGVEVIKQQ KQQVDILFAE NASKEVEIQR LSKIGGQYGR LFGFGMDGSK
     LKIVLYIKDL KPQEWLMILY ETLKELSGVK DEKSIIA
//

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