(data stored in ACNUC7421 zone)

SWISSPROT: A0A0F6BHU7_GEOS0

ID   A0A0F6BHU7_GEOS0        Unreviewed;       248 AA.
AC   A0A0F6BHU7;
DT   24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT   24-JUN-2015, sequence version 1.
DT   08-MAY-2019, entry version 24.
DE   RecName: Full=tRNA pseudouridine synthase A {ECO:0000256|HAMAP-Rule:MF_00171};
DE            EC=5.4.99.12 {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridylate synthase I {ECO:0000256|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA-uridine isomerase I {ECO:0000256|HAMAP-Rule:MF_00171};
GN   Name=truA {ECO:0000256|HAMAP-Rule:MF_00171};
GN   OrderedLocusNames=GY4MC1_0142 {ECO:0000313|EMBL:ADP72998.1};
OS   Geobacillus sp. (strain Y4.1MC1).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=581103 {ECO:0000313|EMBL:ADP72998.1, ECO:0000313|Proteomes:UP000002034};
RN   [1] {ECO:0000313|EMBL:ADP72998.1, ECO:0000313|Proteomes:UP000002034}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y4.1MC1 {ECO:0000313|EMBL:ADP72998.1,
RC   ECO:0000313|Proteomes:UP000002034};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Chertkov O., Zhang X., Detter J.C., Han C., Tapia R.,
RA   Land M., Hauser L., Jeffries C., Kyrpides N., Ivanova N.,
RA   Ovchinnikova G., Brumm P., Mead D., Woyke T.;
RT   "Complete sequence of chromosome of Geobacillus sp. Y4.1MC1.";
RL   Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs. {ECO:0000256|HAMAP-
CC       Rule:MF_00171}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=tRNA uridine(38-40) = tRNA pseudouridine(38-40);
CC         Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-
CC         COMP:10087, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00171,
CC         ECO:0000256|RuleBase:RU003792};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00171}.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA
CC       family. {ECO:0000256|HAMAP-Rule:MF_00171,
CC       ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00543779}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00171}.
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DR   EMBL; CP002293; ADP72998.1; -; Genomic_DNA.
DR   RefSeq; WP_003247630.1; NC_014650.1.
DR   EnsemblBacteria; ADP72998; ADP72998; GY4MC1_0142.
DR   GeneID; 29237222; -.
DR   KEGG; gmc:GY4MC1_0142; -.
DR   KO; K06173; -.
DR   OMA; FLYGMVR; -.
DR   OrthoDB; 1075262at2; -.
DR   BioCyc; GSP581103:G1GOQ-169-MONOMER; -.
DR   Proteomes; UP000002034; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
DR   PRODOM; A0A0F6BHU7.
DR   SWISS-2DPAGE; A0A0F6BHU7.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002034};
KW   Isomerase {ECO:0000256|HAMAP-Rule:MF_00171,
KW   ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00119851};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00171,
KW   ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00427419}.
FT   DOMAIN       10    105       PseudoU_synth_1. {ECO:0000259|Pfam:
FT                                PF01416}.
FT   DOMAIN      146    247       PseudoU_synth_1. {ECO:0000259|Pfam:
FT                                PF01416}.
FT   ACT_SITE     54     54       Nucleophile. {ECO:0000256|HAMAP-Rule:
FT                                MF_00171, ECO:0000256|PIRSR:PIRSR001430-
FT                                1}.
FT   BINDING     112    112       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_00171, ECO:0000256|PIRSR:PIRSR001430-
FT                                2}.
SQ   SEQUENCE   248 AA;  28290 MW;  0B6E63989C7509F8 CRC64;
     MSKRIKCTLS YDGTHFFGYQ IQPGKRTVQG ELEQALEQIH KGKAIRVTAS GRTDAGVHAY
     GQVIHFDTFL SLSPEQWKKA LNALLPDDIV IKDAQEAAPS FHARFSATAK EYRYKVRIAQ
     ERDVFLRHYC YHYPYPLDME AMGRALRLIE GTHDFTSFCS AKTDVDDRVR TIYKADMTVR
     DDLLEFRFIG NGFLYNMVRI IVGTILEVGQ GKRSVDSISS ALAAKDRRFA GKTAPPQGLY
     LWKVYYDN
//

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