(data stored in ACNUC7421 zone)

SWISSPROT: E3J3J6_FRAIE

ID   E3J3J6_FRAIE            Unreviewed;       520 AA.
AC   E3J3J6;
DT   11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT   11-JAN-2011, sequence version 1.
DT   08-MAY-2019, entry version 41.
DE   RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE            Short=P2TMN nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE            EC=4.99.1.12 {ECO:0000256|HAMAP-Rule:MF_01074};
DE   AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000256|HAMAP-Rule:MF_01074};
GN   Name=larC {ECO:0000256|HAMAP-Rule:MF_01074};
GN   OrderedLocusNames=FraEuI1c_0110 {ECO:0000313|EMBL:ADP78198.1};
OS   Frankia inefficax (strain DSM 45817 / CECT 9037 / EuI1c).
OC   Bacteria; Actinobacteria; Frankiales; Frankiaceae; Frankia.
OX   NCBI_TaxID=298654 {ECO:0000313|EMBL:ADP78198.1, ECO:0000313|Proteomes:UP000002484};
RN   [1] {ECO:0000313|EMBL:ADP78198.1, ECO:0000313|Proteomes:UP000002484}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 45817 / CECT 9037 / EuI1c
RC   {ECO:0000313|Proteomes:UP000002484};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M.,
RA   Hauser L., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N.,
RA   Beauchemin N., Sen A., Sur S.A., Gtari M., Wall L., Tisa L., Woyke T.;
RT   "Complete sequence of Frankia sp. EuI1c.";
RL   Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC       ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in
CC       nickel delivery to pyridinium-3,5-bisthiocarboxylic acid
CC       mononucleotide (P2TMN), to form the mature cofactor. Is thus
CC       probably required for the activation of nickel-pincer cofactor-
CC       dependent enzymes. {ECO:0000256|HAMAP-Rule:MF_01074}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC         Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC         Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC         ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01074};
CC   -!- SIMILARITY: Belongs to the LarC family. {ECO:0000256|HAMAP-
CC       Rule:MF_01074}.
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DR   EMBL; CP002299; ADP78198.1; -; Genomic_DNA.
DR   RefSeq; WP_013421321.1; NC_014666.1.
DR   STRING; 298654.FraEuI1c_0110; -.
DR   EnsemblBacteria; ADP78198; ADP78198; FraEuI1c_0110.
DR   KEGG; fri:FraEuI1c_0110; -.
DR   eggNOG; ENOG4105C8M; Bacteria.
DR   eggNOG; COG1641; LUCA.
DR   HOGENOM; HOG000007120; -.
DR   KO; K09121; -.
DR   OMA; TCLGLDW; -.
DR   OrthoDB; 1547060at2; -.
DR   BioCyc; FSP298654:G1GOT-112-MONOMER; -.
DR   Proteomes; UP000002484; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051188; P:cofactor biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01074; LarC; 1.
DR   InterPro; IPR002822; Ni_insertion.
DR   PANTHER; PTHR36566; PTHR36566; 1.
DR   Pfam; PF01969; DUF111; 1.
PE   3: Inferred from homology;
DR   PRODOM; E3J3J6.
DR   SWISS-2DPAGE; E3J3J6.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002484};
KW   Lyase {ECO:0000256|HAMAP-Rule:MF_01074};
KW   Nickel {ECO:0000256|HAMAP-Rule:MF_01074};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002484}.
SQ   SEQUENCE   520 AA;  52861 MW;  AC1B530A9BD9C2DB CRC64;
     MSGPAPAAAG RVGWLDLSCG VSGDMLLGAL VDAGVPLAVP AAAIDTLGLP IRLEARQVRR
     SGLAATKVDV IAPDGGHSRT WADIRELLAA APLADATRDL ATATFAALAA AEARVHGIDP
     AEVHFHEVGA LDAIADIVGV SAGFAHLALA GLVATPIALG GGRARTAHGV LPIPGPAVLE
     LLRAAGAPAL GGPVAVELCT PTGAALVVTV ASGFGDLPAV RVTAVGCGAG TRDLPGRPNL
     VRLVVGDALD ATAVPPPVPR PQHAAHDHLA HDHQLAHDRQ LAHDHAGHRH AEAEPHRHDP
     RPDRATGDAN AQGAGQPTAV AGIDVADGVA VTAELVLEAN VDDLDPRVWP SVLAALLAAG
     AADAWLTPIL MKKGRPAHTL RALVAPGRLP AVRAAIFRHT STLGLREIPV RKHALARSMR
     AVEVAGHPVR VKVSPGPDRA FDQPAGPVPV AQPEWEDVAL VAEKLGWPAR EVLAAACALA
     YAGQWSDLPP TADGPDDHQH GTEPAGQGTG DQGVDPGHAP
//

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