(data stored in ACNUC1104 zone)

SWISSPROT: E6UZ04_VARPE

ID   E6UZ04_VARPE            Unreviewed;       429 AA.
AC   E6UZ04;
DT   08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT   08-MAR-2011, sequence version 1.
DT   08-MAY-2019, entry version 50.
DE   RecName: Full=Benzoyl-CoA oxygenase component A {ECO:0000256|PIRNR:PIRNR000361};
DE            EC=1.14.13.208 {ECO:0000256|PIRNR:PIRNR000361};
GN   OrderedLocusNames=Varpa_0111 {ECO:0000313|EMBL:ADU34333.1};
OS   Variovorax paradoxus (strain EPS).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Variovorax.
OX   NCBI_TaxID=595537 {ECO:0000313|EMBL:ADU34333.1, ECO:0000313|Proteomes:UP000008917};
RN   [1] {ECO:0000313|Proteomes:UP000008917}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EPS {ECO:0000313|Proteomes:UP000008917};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L.,
RA   Pitluck S., Teshima H., Detter J.C., Han C., Tapia R., Land M.,
RA   Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Orwin P.,
RA   Han J.-I.G., Woyke T.;
RT   "Complete sequence of Variovorax paradoxus EPS.";
RL   Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADU34333.1, ECO:0000313|Proteomes:UP000008917}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EPS {ECO:0000313|EMBL:ADU34333.1,
RC   ECO:0000313|Proteomes:UP000008917};
RX   PubMed=24158554;
RA   Han J.I., Spain J.C., Leadbetter J.R., Ovchinnikova G., Goodwin L.A.,
RA   Han C.S., Woyke T., Davenport K.W., Orwin P.M.;
RT   "Genome of the Root-Associated Plant Growth-Promoting Bacterium
RT   Variovorax paradoxus Strain EPS.";
RL   Genome Announc. 1:e00843-13(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=benzoyl-CoA + H(+) + NADPH + O2 = 2,3-epoxy-2,3-
CC         dihydrobenzoyl-CoA + H2O + NADP(+); Xref=Rhea:RHEA:48312,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:57369, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:88118; EC=1.14.13.208;
CC         Evidence={ECO:0000256|PIRNR:PIRNR000361};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|PIRNR:PIRNR000361}.
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DR   EMBL; CP002417; ADU34333.1; -; Genomic_DNA.
DR   RefSeq; WP_013538580.1; NC_014931.1.
DR   STRING; 595537.Varpa_0111; -.
DR   EnsemblBacteria; ADU34333; ADU34333; Varpa_0111.
DR   GeneID; 29716716; -.
DR   KEGG; vpe:Varpa_0111; -.
DR   eggNOG; ENOG4107VFH; Bacteria.
DR   eggNOG; COG0369; LUCA.
DR   HOGENOM; HOG000220125; -.
DR   KO; K15511; -.
DR   OMA; GRMYIQD; -.
DR   OrthoDB; 707164at2; -.
DR   BioCyc; VPAR595537:G1GQO-108-MONOMER; -.
DR   Proteomes; UP000008917; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.80; -; 1.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR017634; Benzoyl_CoA_Oase_BoxA.
DR   InterPro; IPR017927; FAD-bd_FR_type.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR015701; FNR.
DR   InterPro; IPR039261; FNR_nucleotide-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR   InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR   Pfam; PF00037; Fer4; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PIRSF; PIRSF501177; BoxA; 1.
DR   PIRSF; PIRSF000361; Frd-NADP+_RD; 1.
DR   PRINTS; PR00371; FPNCR.
DR   SUPFAM; SSF52343; SSF52343; 1.
DR   SUPFAM; SSF63380; SSF63380; 1.
DR   TIGRFAMs; TIGR03224; benzo_boxA; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 2.
DR   PROSITE; PS51379; 4FE4S_FER_2; 2.
DR   PROSITE; PS51384; FAD_FR; 1.
PE   4: Predicted;
DR   PRODOM; E6UZ04.
DR   SWISS-2DPAGE; E6UZ04.
KW   Complete proteome {ECO:0000313|Proteomes:UP000008917};
KW   FAD {ECO:0000256|PIRNR:PIRNR000361};
KW   Flavoprotein {ECO:0000256|PIRNR:PIRNR000361};
KW   NADP {ECO:0000256|PIRNR:PIRNR000361, ECO:0000256|PIRSR:PIRSR000361-1};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR000361};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008917}.
FT   DOMAIN       11     40       4Fe-4S ferredoxin-type.
FT                                {ECO:0000259|PROSITE:PS51379}.
FT   DOMAIN       42     69       4Fe-4S ferredoxin-type.
FT                                {ECO:0000259|PROSITE:PS51379}.
FT   DOMAIN      156    278       FAD-binding FR-type.
FT                                {ECO:0000259|PROSITE:PS51384}.
FT   NP_BIND     352    353       NADP. {ECO:0000256|PIRSR:PIRSR000361-1}.
FT   NP_BIND     388    389       NADP. {ECO:0000256|PIRSR:PIRSR000361-1}.
FT   BINDING     218    218       NADP. {ECO:0000256|PIRSR:PIRSR000361-1}.
FT   BINDING     238    238       NADP. {ECO:0000256|PIRSR:PIRSR000361-1}.
FT   BINDING     293    293       NADP; via amide nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR000361-1}.
FT   BINDING     359    359       NADP. {ECO:0000256|PIRSR:PIRSR000361-1}.
FT   BINDING     427    427       NADP. {ECO:0000256|PIRSR:PIRSR000361-1}.
SQ   SEQUENCE   429 AA;  46587 MW;  6F60D4383D15F985 CRC64;
     MDMAVEAGVI KQHLIDPEIC IRCNTCEATC PVNAITHDDN NYVVRADVCN GCMACISPCP
     TGSIDNWRTM PLVRAYTIEE QLTWEELPAE LTPEELEAAG ISADVGDEAG ASAAPSPTQA
     QAAVEPIEAV FNSAQYGATV PPWSAAHAYT NLFPPKTPTI ATVVGNFNCT EAGFESETHH
     IVLDFGVVPF PVLEGQSIGI VPPGVDAIGK RHHARQYSVA SPRNGERPGY NNVSLTVKRV
     TEDHQGDPVL GVCSNYVCDL KVGDTVQVVG PFGASFLMPN HPKSHIVMIC TGTGSAPMRA
     MTEWRRRLRK SGKFEGGKLM LFFGARTQQE LPYFGPLQSL PKDFIDINLA FSRTPGQPKR
     YVQDLMRERA ADLAALLKDG TSHFYVCGLK SMEEGVVLAL RDVATEAGLD WDTLGASLKR
     EGRLHLETY
//

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