(data stored in ACNUC1104 zone)

SWISSPROT: E6V3F1_VARPE

ID   E6V3F1_VARPE            Unreviewed;       497 AA.
AC   E6V3F1;
DT   08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT   08-MAR-2011, sequence version 1.
DT   08-MAY-2019, entry version 48.
DE   RecName: Full=NAD(P) transhydrogenase subunit beta {ECO:0000256|PIRNR:PIRNR000204};
DE            EC=7.1.1.1 {ECO:0000256|PIRNR:PIRNR000204};
DE   AltName: Full=Nicotinamide nucleotide transhydrogenase subunit beta {ECO:0000256|PIRNR:PIRNR000204};
GN   OrderedLocusNames=Varpa_0419 {ECO:0000313|EMBL:ADU34641.1};
OS   Variovorax paradoxus (strain EPS).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Variovorax.
OX   NCBI_TaxID=595537 {ECO:0000313|EMBL:ADU34641.1, ECO:0000313|Proteomes:UP000008917};
RN   [1] {ECO:0000313|Proteomes:UP000008917}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EPS {ECO:0000313|Proteomes:UP000008917};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L.,
RA   Pitluck S., Teshima H., Detter J.C., Han C., Tapia R., Land M.,
RA   Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Orwin P.,
RA   Han J.-I.G., Woyke T.;
RT   "Complete sequence of Variovorax paradoxus EPS.";
RL   Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADU34641.1, ECO:0000313|Proteomes:UP000008917}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EPS {ECO:0000313|EMBL:ADU34641.1,
RC   ECO:0000313|Proteomes:UP000008917};
RX   PubMed=24158554;
RA   Han J.I., Spain J.C., Leadbetter J.R., Ovchinnikova G., Goodwin L.A.,
RA   Han C.S., Woyke T., Davenport K.W., Orwin P.M.;
RT   "Genome of the Root-Associated Plant Growth-Promoting Bacterium
RT   Variovorax paradoxus Strain EPS.";
RL   Genome Announc. 1:e00843-13(2013).
CC   -!- FUNCTION: The transhydrogenation between NADH and NADP is coupled
CC       to respiration and ATP hydrolysis and functions as a proton pump
CC       across the membrane. {ECO:0000256|PIRNR:PIRNR000204}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+)(in) + NAD(+) + NADPH = H(+)(out) + NADH + NADP(+);
CC         Xref=Rhea:RHEA:47992, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:57945, ChEBI:CHEBI:58349;
CC         EC=7.1.1.1; Evidence={ECO:0000256|PIRNR:PIRNR000204};
CC   -!- SIMILARITY: Belongs to the PNT beta subunit family.
CC       {ECO:0000256|PIRNR:PIRNR000204}.
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DR   EMBL; CP002417; ADU34641.1; -; Genomic_DNA.
DR   STRING; 595537.Varpa_0419; -.
DR   EnsemblBacteria; ADU34641; ADU34641; Varpa_0419.
DR   KEGG; vpe:Varpa_0419; -.
DR   eggNOG; ENOG4105C15; Bacteria.
DR   eggNOG; COG1282; LUCA.
DR   HOGENOM; HOG000243958; -.
DR   KO; K00325; -.
DR   OMA; AMTSMPE; -.
DR   Proteomes; UP000008917; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008750; F:NAD(P)+ transhydrogenase (AB-specific) activity; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   InterPro; IPR012136; NADH_DH_b.
DR   InterPro; IPR034300; PNTB-like.
DR   Pfam; PF02233; PNTB; 1.
DR   PIRSF; PIRSF000204; PNTB; 1.
DR   SUPFAM; SSF52467; SSF52467; 1.
PE   3: Inferred from homology;
DR   PRODOM; E6V3F1.
DR   SWISS-2DPAGE; E6V3F1.
KW   Cell inner membrane {ECO:0000256|PIRNR:PIRNR000204};
KW   Cell membrane {ECO:0000256|PIRNR:PIRNR000204};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008917};
KW   Membrane {ECO:0000256|PIRNR:PIRNR000204, ECO:0000256|SAM:Phobius};
KW   NAD {ECO:0000256|PIRNR:PIRNR000204};
KW   NADP {ECO:0000256|PIRNR:PIRNR000204};
KW   Oxidoreductase {ECO:0000313|EMBL:ADU34641.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008917};
KW   Translocase {ECO:0000256|PIRNR:PIRNR000204};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM      6     22       Helical. {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM     34     52       Helical. {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM     64     82       Helical. {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM     94    115       Helical. {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM    158    179       Helical. {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM    200    218       Helical. {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM    224    243       Helical. {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM    274    294       Helical. {ECO:0000256|SAM:Phobius}.
FT   DOMAIN        5    493       PNTB. {ECO:0000259|Pfam:PF02233}.
SQ   SEQUENCE   497 AA;  51757 MW;  18C20C17F1526F14 CRC64;
     MNLVTLLYLV ASVCFIQALK GLSHPTTSIR GNIFGMTGMA IAVLTTIALI HGQARSLNVD
     FGTGLAWVLA AVVVGGGLGA FMANKVEMTK MPELVAFMHS MIGLAAVFIG VAAVAEPWAF
     GITAAPVAAL IGAQTPDGAV ILDGFVRYAI PAGNRLELFL GAAIGAITFS GSVIAFGKLS
     GKYKFRLFQG APVQFSGQHM LNLVLGLLTV ALGLVFVFTE SWPAFFAMLA LAFVMGVLII
     IPIGGADMPV VVSMLNSYSG WAAAGIGFSL NNAMLIVAGS LVGSSGAILS YIMCKAMNRS
     FFNVILGGFG GEAATTGGAA KEQRPVKTGS ADDAAFVLGN AETVVIVPGY GLAVARAQHA
     VKELAAKLTE KGITVKYAIH PVAGRMPGHM NVLLAEAEVP YDQVFEMEDI NGEFGQADVA
     IILGANDVVN PAAHTKGSPI YGMPILEAYK AKTVIVNKRS MAAGYAGLDN ELFYMDKTMM
     VFGDAKKVVE DMGKAIE
//

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