(data stored in ACNUC7421 zone)

SWISSPROT: E6S842_INTC7

ID   E6S842_INTC7            Unreviewed;       213 AA.
AC   E6S842;
DT   08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT   08-MAR-2011, sequence version 1.
DT   08-MAY-2019, entry version 58.
DE   RecName: Full=Uracil phosphoribosyltransferase {ECO:0000256|HAMAP-Rule:MF_01218, ECO:0000256|SAAS:SAAS00709136};
DE            EC=2.4.2.9 {ECO:0000256|HAMAP-Rule:MF_01218, ECO:0000256|SAAS:SAAS00918612};
DE   AltName: Full=UMP pyrophosphorylase {ECO:0000256|HAMAP-Rule:MF_01218};
DE   AltName: Full=UPRTase {ECO:0000256|HAMAP-Rule:MF_01218};
GN   Name=upp {ECO:0000256|HAMAP-Rule:MF_01218};
GN   OrderedLocusNames=Intca_0397 {ECO:0000313|EMBL:ADU46946.1};
OS   Intrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 /
OS   NBRC 12989 / 7 KIP).
OC   Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
OC   Intrasporangium.
OX   NCBI_TaxID=710696 {ECO:0000313|EMBL:ADU46946.1, ECO:0000313|Proteomes:UP000008914};
RN   [1] {ECO:0000313|EMBL:ADU46946.1, ECO:0000313|Proteomes:UP000008914}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP
RC   {ECO:0000313|Proteomes:UP000008914};
RX   PubMed=21304734; DOI=10.4056/sigs.1263355;
RA   Del Rio T.G., Chertkov O., Yasawong M., Lucas S., Deshpande S.,
RA   Cheng J.F., Detter C., Tapia R., Han C., Goodwin L., Pitluck S.,
RA   Liolios K., Ivanova N., Mavromatis K., Pati A., Chen A.,
RA   Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D.,
RA   Rohde M., Pukall R., Sikorski J., Goker M., Woyke T., Bristow J.,
RA   Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.,
RA   Lapidus A.;
RT   "Complete genome sequence of Intrasporangium calvum type strain (7
RT   KIP).";
RL   Stand. Genomic Sci. 3:294-303(2010).
CC   -!- FUNCTION: Catalyzes the conversion of uracil and 5-phospho-alpha-
CC       D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.
CC       {ECO:0000256|HAMAP-Rule:MF_01218, ECO:0000256|SAAS:SAAS00709092}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + UMP = 5-phospho-alpha-D-ribose 1-
CC         diphosphate + uracil; Xref=Rhea:RHEA:13017, ChEBI:CHEBI:17568,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57865, ChEBI:CHEBI:58017;
CC         EC=2.4.2.9; Evidence={ECO:0000256|HAMAP-Rule:MF_01218,
CC         ECO:0000256|SAAS:SAAS01124291};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01218};
CC       Note=Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-
CC       PRPP. {ECO:0000256|HAMAP-Rule:MF_01218};
CC   -!- ACTIVITY REGULATION: Allosterically activated by GTP.
CC       {ECO:0000256|HAMAP-Rule:MF_01218, ECO:0000256|SAAS:SAAS01124293}.
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via salvage
CC       pathway; UMP from uracil: step 1/1. {ECO:0000256|HAMAP-
CC       Rule:MF_01218, ECO:0000256|SAAS:SAAS00918634}.
CC   -!- SIMILARITY: Belongs to the UPRTase family. {ECO:0000256|HAMAP-
CC       Rule:MF_01218, ECO:0000256|SAAS:SAAS00918654}.
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DR   EMBL; CP002343; ADU46946.1; -; Genomic_DNA.
DR   RefSeq; WP_013491267.1; NC_014830.1.
DR   STRING; 710696.Intca_0397; -.
DR   EnsemblBacteria; ADU46946; ADU46946; Intca_0397.
DR   KEGG; ica:Intca_0397; -.
DR   eggNOG; ENOG4105CZ5; Bacteria.
DR   eggNOG; COG0035; LUCA.
DR   HOGENOM; HOG000262754; -.
DR   KO; K00761; -.
DR   OrthoDB; 1581906at2; -.
DR   UniPathway; UPA00574; UER00636.
DR   Proteomes; UP000008914; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004845; F:uracil phosphoribosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009116; P:nucleoside metabolic process; IEA:InterPro.
DR   GO; GO:0044206; P:UMP salvage; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006223; P:uracil salvage; IEA:InterPro.
DR   CDD; cd06223; PRTases_typeI; 1.
DR   HAMAP; MF_01218_B; Upp_B; 1.
DR   InterPro; IPR000836; PRibTrfase_dom.
DR   InterPro; IPR029057; PRTase-like.
DR   InterPro; IPR034332; Upp_B.
DR   InterPro; IPR005765; Ura_phspho_trans.
DR   SUPFAM; SSF53271; SSF53271; 1.
DR   TIGRFAMs; TIGR01091; upp; 1.
PE   3: Inferred from homology;
DR   PRODOM; E6S842.
DR   SWISS-2DPAGE; E6S842.
KW   Allosteric enzyme {ECO:0000256|HAMAP-Rule:MF_01218};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008914};
KW   Glycosyltransferase {ECO:0000256|HAMAP-Rule:MF_01218,
KW   ECO:0000256|SAAS:SAAS00918621, ECO:0000313|EMBL:ADU46946.1};
KW   GTP-binding {ECO:0000256|HAMAP-Rule:MF_01218,
KW   ECO:0000256|SAAS:SAAS00918618};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_01218,
KW   ECO:0000256|SAAS:SAAS00709102};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01218,
KW   ECO:0000256|SAAS:SAAS00918615};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008914};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_01218,
KW   ECO:0000256|SAAS:SAAS00918631, ECO:0000313|EMBL:ADU46946.1}.
FT   REGION      130    138       5-phospho-alpha-D-ribose 1-diphosphate
FT                                binding. {ECO:0000256|HAMAP-Rule:
FT                                MF_01218}.
FT   REGION      202    204       Uracil binding. {ECO:0000256|HAMAP-Rule:
FT                                MF_01218}.
FT   BINDING      78     78       5-phospho-alpha-D-ribose 1-diphosphate.
FT                                {ECO:0000256|HAMAP-Rule:MF_01218}.
FT   BINDING     103    103       5-phospho-alpha-D-ribose 1-diphosphate.
FT                                {ECO:0000256|HAMAP-Rule:MF_01218}.
FT   BINDING     197    197       Uracil; via amide nitrogen.
FT                                {ECO:0000256|HAMAP-Rule:MF_01218}.
FT   BINDING     203    203       5-phospho-alpha-D-ribose 1-diphosphate.
FT                                {ECO:0000256|HAMAP-Rule:MF_01218}.
SQ   SEQUENCE   213 AA;  23130 MW;  ABE4E012A01FBE5D CRC64;
     MDVHVADHPL IAHKLTYLRD KRTDSPTFRR LAEELMTLLA YEATREVRVE PFQIETPVTA
     TTGIKLSNPK PLVVPILRAG LGMLEGMVRL LPSAEVGFLG MQRNEVTLEA VTYANRLPDD
     LTGRQVFVLD PMLATGGSMS DAIHFLIDRG ADDITAVCLL AAPEGIAHLE ESLASTNVPV
     RVVTGAVDER LNEKGYIVPG LGDAGDRLYG TVD
//

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