(data stored in ACNUC7421 zone)

SWISSPROT: E6S9A7_INTC7

ID   E6S9A7_INTC7            Unreviewed;      1020 AA.
AC   E6S9A7;
DT   08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT   08-MAR-2011, sequence version 1.
DT   08-MAY-2019, entry version 51.
DE   RecName: Full=Multifunctional fusion protein {ECO:0000256|HAMAP-Rule:MF_00254, ECO:0000256|HAMAP-Rule:MF_00255};
DE   Includes:
DE     RecName: Full=Glycine--tRNA ligase alpha subunit {ECO:0000256|HAMAP-Rule:MF_00254};
DE              EC=6.1.1.14 {ECO:0000256|HAMAP-Rule:MF_00254};
DE     AltName: Full=Glycyl-tRNA synthetase alpha subunit {ECO:0000256|HAMAP-Rule:MF_00254};
DE              Short=GlyRS {ECO:0000256|HAMAP-Rule:MF_00254};
DE   Includes:
DE     RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE     AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
GN   Name=glyQ {ECO:0000256|HAMAP-Rule:MF_00254};
GN   Synonyms=glyS {ECO:0000256|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=Intca_0538 {ECO:0000313|EMBL:ADU47083.1};
OS   Intrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 /
OS   NBRC 12989 / 7 KIP).
OC   Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
OC   Intrasporangium.
OX   NCBI_TaxID=710696 {ECO:0000313|EMBL:ADU47083.1, ECO:0000313|Proteomes:UP000008914};
RN   [1] {ECO:0000313|EMBL:ADU47083.1, ECO:0000313|Proteomes:UP000008914}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP
RC   {ECO:0000313|Proteomes:UP000008914};
RX   PubMed=21304734; DOI=10.4056/sigs.1263355;
RA   Del Rio T.G., Chertkov O., Yasawong M., Lucas S., Deshpande S.,
RA   Cheng J.F., Detter C., Tapia R., Han C., Goodwin L., Pitluck S.,
RA   Liolios K., Ivanova N., Mavromatis K., Pati A., Chen A.,
RA   Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D.,
RA   Rohde M., Pukall R., Sikorski J., Goker M., Woyke T., Bristow J.,
RA   Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.,
RA   Lapidus A.;
RT   "Complete genome sequence of Intrasporangium calvum type strain (7
RT   KIP).";
RL   Stand. Genomic Sci. 3:294-303(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:57305, ChEBI:CHEBI:78442, ChEBI:CHEBI:78522,
CC         ChEBI:CHEBI:456215; EC=6.1.1.14; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00254, ECO:0000256|SAAS:SAAS01125800};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000256|HAMAP-Rule:MF_00254}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00254}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC       family. {ECO:0000256|HAMAP-Rule:MF_00254,
CC       ECO:0000256|SAAS:SAAS00578611}.
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DR   EMBL; CP002343; ADU47083.1; -; Genomic_DNA.
DR   STRING; 710696.Intca_0538; -.
DR   EnsemblBacteria; ADU47083; ADU47083; Intca_0538.
DR   KEGG; ica:Intca_0538; -.
DR   eggNOG; ENOG4105C38; Bacteria.
DR   eggNOG; COG0751; LUCA.
DR   eggNOG; COG0752; LUCA.
DR   HOGENOM; HOG000264303; -.
DR   KO; K14164; -.
DR   BioCyc; ICAL710696:GH9U-551-MONOMER; -.
DR   Proteomes; UP000008914; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd00733; GlyRS_alpha_core; 1.
DR   HAMAP; MF_00254; Gly_tRNA_synth_alpha; 1.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   InterPro; IPR002310; Gly-tRNA_ligase_asu.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02091; tRNA-synt_2e; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01044; TRNASYNTHGA.
DR   TIGRFAMs; TIGR00388; glyQ; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 2.
PE   3: Inferred from homology;
DR   PRODOM; E6S9A7.
DR   SWISS-2DPAGE; E6S9A7.
KW   Aminoacyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00254,
KW   ECO:0000256|SAAS:SAAS00470070, ECO:0000313|EMBL:ADU47083.1};
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_00254,
KW   ECO:0000256|SAAS:SAAS00470125};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008914};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00254};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_00254,
KW   ECO:0000256|SAAS:SAAS00514596, ECO:0000313|EMBL:ADU47083.1};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00254,
KW   ECO:0000256|SAAS:SAAS00470150};
KW   Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00254,
KW   ECO:0000256|SAAS:SAAS00470089};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008914}.
SQ   SEQUENCE   1020 AA;  110616 MW;  05BEEE1C880CABFD CRC64;
     MPDTTTLTVQ DALLTLQKFW TDRGCMVVQP FNTEVGAGTM NPATILRVLG PEPWRVAYVE
     PSVRPDDSRY GENPNRLQQH TQFQVILKPD PGNPQELYLE SLEALGIDIR AHDIRFVEDN
     WAQPAIGAWG LGWEVWLDGM EITQFTYFQQ VGGQNLDPVA VELTYGVERI MMALQGVAHF
     KDMVYAPGIT YGEAFGQSEY EMSRYYLDDA DIQTNRDLFE RFTAEAQRLI DVRLPVPAHT
     YVLKSSHAFN VLDARGAIST TERAKAFGTM RRLAREVSRL WVERREELGF PLLGADLPPM
     EGRALMPPSQ GVEVTPEAYA AELASLAPDV TRRLALEIGV EELPPHVVPE AIEQVRTALT
     QKLGATRLPH GSVVVQGTPR RIIALVDAVG VHEPDAETLR KGPKVAAAFG PDGAATKAAE
     GFARGQKVDV SDLVRAEFDG VEHVAVRVAE TGRGVLEVLG SIIADVVAGL RADKNMRWSD
     PELAYSRPIR WIVALWGDTV VPVTVSGLRS GRTTYGERTE AGPLIEVENA DVLLPKLAER
     DFIVDPVARR ESVVSQAKSL AASVGGAVDV EGEAALVDEI THLVEAPHGI LGSFEERYLE
     LPEQILTTVM RKHQRYLPVR SGDGALLPYF VTMANGECDD DLVRKGNENV LRARYEDAAF
     FYAADLKVPL EELRAGIAKL TFEERIGSVA DRADRIRDVA AALAELVGLD ASEGATLARA
     GQLAKFDLAS QMVVELSSLA GFMAREYALK AGETPEVAAA LYEMEQPRTA GGPLPASRAG
     AVLALADRFD LLMAMFALGA KPTGSSDPFA LRRAALGVIG ILRSQPTLAG LTIEQGLHAA
     ADQLRQQGLD VSAEAIADAV EFVTGRFGQQ LREEGVPSTL AGAVQPLAGT PGKAEVALRD
     IETVRGREGF AKLVEATQRI TRIVPEGSPA AYDPQVLREE AERELSVALE TIPDLSGAAL
     PEWAGVAVRA VPALERFFDE VLVMAEDPAL RAARLGLLQT FVARAPRGID WRALHSALAG
//

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