(data stored in ACNUC13479 zone)

SWISSPROT: PTR5_ARATH

ID   PTR5_ARATH              Reviewed;         570 AA.
AC   Q9LFB8; Q0WR84; Q84TJ5;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   07-JUN-2017, entry version 102.
DE   RecName: Full=Protein NRT1/ PTR FAMILY 8.2;
DE            Short=AtNPF8.2;
DE   AltName: Full=Peptide transporter PTR5;
GN   Name=NPF8.2; Synonyms=PTR5; OrderedLocusNames=At5g01180;
GN   ORFNames=F7J8.160;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Portal (Araport);
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 59-570.
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 60-570.
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA   Hayashizaki Y., Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   TISSUE SPECIFICITY, AND GENE FAMILY.
RX   PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA   Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT   "Nitrate transporters and peptide transporters.";
RL   FEBS Lett. 581:2290-2300(2007).
RN   [6]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP   STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=18753286; DOI=10.1104/pp.108.123844;
RA   Komarova N.Y., Thor K., Gubler A., Meier S., Dietrich D., Weichert A.,
RA   Suter Grotemeyer M., Tegeder M., Rentsch D.;
RT   "AtPTR1 and AtPTR5 transport dipeptides in planta.";
RL   Plant Physiol. 148:856-869(2008).
RN   [7]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA   Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA   Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA   Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A.,
RA   Harris J.M., Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F.,
RA   Zhang M., Coruzzi G., Lacombe B.;
RT   "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER
RT   family members in plants.";
RL   Trends Plant Sci. 19:5-9(2014).
CC   -!- FUNCTION: Peptide transporter. Mediates the transport of di- and
CC       tripeptides. High affinity transporter. Involved in the uptake of
CC       peptides during pollen germination and tube growth.
CC       {ECO:0000269|PubMed:18753286}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18753286};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:18753286}.
CC   -!- TISSUE SPECIFICITY: Expressed in developing and germinating pollen
CC       grains and ovules. {ECO:0000269|PubMed:17481610,
CC       ECO:0000269|PubMed:18753286}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during early seed development.
CC       {ECO:0000269|PubMed:18753286}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC       {ECO:0000269|PubMed:18753286}.
CC   -!- SIMILARITY: Belongs to the PTR2/POT transporter (TC 2.A.17)
CC       family. {ECO:0000305}.
DR   EMBL; AL137189; CAB69846.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED90307.1; -; Genomic_DNA.
DR   EMBL; CP002688; ANM69606.1; -; Genomic_DNA.
DR   EMBL; CP002688; ANM69607.1; -; Genomic_DNA.
DR   EMBL; BT005728; AAO64143.1; -; mRNA.
DR   EMBL; AK228435; BAF00365.1; -; mRNA.
DR   PIR; T45958; T45958.
DR   RefSeq; NP_001331271.1; NM_001342576.1.
DR   RefSeq; NP_001331272.1; NM_001342577.1.
DR   RefSeq; NP_195738.1; NM_120196.4.
DR   UniGene; At.33937; -.
DR   ProteinModelPortal; Q9LFB8; -.
DR   BioGrid; 17001; 25.
DR   STRING; 3702.AT5G01180.1; -.
DR   TCDB; 2.A.17.3.8; the proton-dependent oligopeptide transporter (pot/ptr) family.
DR   PaxDb; Q9LFB8; -.
DR   EnsemblPlants; AT5G01180.1; AT5G01180.1; AT5G01180.
DR   EnsemblPlants; AT5G01180.2; AT5G01180.2; AT5G01180.
DR   EnsemblPlants; AT5G01180.3; AT5G01180.3; AT5G01180.
DR   GeneID; 831725; -.
DR   Gramene; AT5G01180.1; AT5G01180.1; AT5G01180.
DR   Gramene; AT5G01180.2; AT5G01180.2; AT5G01180.
DR   Gramene; AT5G01180.3; AT5G01180.3; AT5G01180.
DR   KEGG; ath:AT5G01180; -.
DR   Araport; AT5G01180; -.
DR   TAIR; locus:2150124; AT5G01180.
DR   eggNOG; KOG1237; Eukaryota.
DR   eggNOG; COG3104; LUCA.
DR   HOGENOM; HOG000237400; -.
DR   InParanoid; Q9LFB8; -.
DR   OMA; RSMCMAL; -.
DR   OrthoDB; EOG093606MH; -.
DR   PhylomeDB; Q9LFB8; -.
DR   BRENDA; 3.6.3.43; 399.
DR   Reactome; R-ATH-427975; Proton/oligopeptide cotransporters.
DR   Reactome; R-ATH-6798695; Neutrophil degranulation.
DR   PRO; PR:Q9LFB8; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LFB8; baseline and differential.
DR   Genevisible; Q9LFB8; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR   GO; GO:0042936; F:dipeptide transporter activity; IDA:TAIR.
DR   GO; GO:0042938; P:dipeptide transport; IDA:TAIR.
DR   GO; GO:0009860; P:pollen tube growth; IMP:TAIR.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   CDD; cd06174; MFS; 1.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR000109; POT_fam.
DR   InterPro; IPR018456; PTR2_symporter_CS.
DR   PANTHER; PTHR11654; PTHR11654; 1.
DR   Pfam; PF00854; PTR2; 1.
DR   SUPFAM; SSF103473; SSF103473; 2.
DR   PROSITE; PS01022; PTR2_1; 1.
DR   PROSITE; PS01023; PTR2_2; 1.
PE   2: Evidence at transcript level;
DR   PRODOM; Q9LFB8.
DR   SWISS-2DPAGE; Q9LFB8.
KW   Cell membrane; Complete proteome; Membrane; Peptide transport;
KW   Phosphoprotein; Protein transport; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN         1    570       Protein NRT1/ PTR FAMILY 8.2.
FT                                /FTId=PRO_0000378323.
FT   TRANSMEM    100    120       Helical. {ECO:0000255}.
FT   TRANSMEM    136    156       Helical. {ECO:0000255}.
FT   TRANSMEM    182    202       Helical. {ECO:0000255}.
FT   TRANSMEM    210    230       Helical. {ECO:0000255}.
FT   TRANSMEM    335    355       Helical. {ECO:0000255}.
FT   TRANSMEM    370    390       Helical. {ECO:0000255}.
FT   TRANSMEM    414    434       Helical. {ECO:0000255}.
FT   TRANSMEM    454    474       Helical. {ECO:0000255}.
FT   TRANSMEM    493    513       Helical. {ECO:0000255}.
FT   TRANSMEM    537    557       Helical. {ECO:0000255}.
FT   MOD_RES      99     99       Phosphothreonine.
FT                                {ECO:0000250|UniProtKB:Q05085}.
FT   CONFLICT    302    302       T -> A (in Ref. 3; AAO64143 and 4;
FT                                BAF00365). {ECO:0000305}.
SQ   SEQUENCE   570 AA;  63308 MW;  4603B9B64BB20CF5 CRC64;
     MEDDKDIYTK DGTLDIHKKP ANKNKTGTWK ACRFILGTEC CERLAYYGMS TNLINYLEKQ
     MNMENVSASK SVSNWSGTCY ATPLIGAFIA DAYLGRYWTI ASFVVIYIAG MTLLTISASV
     PGLTPTCSGE TCHATAGQTA ITFIALYLIA LGTGGIKPCV SSFGADQFDD TDEKEKESKS
     SFFNWFYFVI NVGAMIASSV LVWIQMNVGW GWGLGVPTVA MAIAVVFFFA GSNFYRLQKP
     GGSPLTRMLQ VIVASCRKSK VKIPEDESLL YENQDAESSI IGSRKLEHTK ILTFFDKAAV
     ETESDNKGAA KSSSWKLCTV TQVEELKALI RLLPIWATGI VFASVYSQMG TVFVLQGNTL
     DQHMGPNFKI PSASLSLFDT LSVLFWAPVY DKLIVPFARK YTGHERGFTQ LQRIGIGLVI
     SIFSMVSAGI LEVARLNYVQ THNLYNEETI PMTIFWQVPQ YFLVGCAEVF TFIGQLEFFY
     DQAPDAMRSL CSALSLTAIA FGNYLSTFLV TLVTKVTRSG GRPGWIAKNL NNGHLDYFFW
     LLAGLSFLNF LVYLWIAKWY TYKKTTGHAL
//

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