(data stored in ACNUC13479 zone)

SWISSPROT: CAX4_ARATH

ID   CAX4_ARATH              Reviewed;         446 AA.
AC   Q945S5; F4K9E5; Q9M025;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 2.
DT   30-AUG-2017, entry version 97.
DE   RecName: Full=Vacuolar cation/proton exchanger 4;
DE            Short=AtCAX4;
DE   AltName: Full=Ca(2+)/H(+) antiporter CAX4;
DE   AltName: Full=Ca(2+)/H(+) exchanger 4;
DE   AltName: Full=Protein CATION EXCHANGER 4;
GN   Name=CAX4; OrderedLocusNames=At5g01490; ORFNames=F7A7.10;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, INDUCTION, AND MUTAGENESIS OF 88-VAL--ARG-96.
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=11950973; DOI=10.1104/pp.010857;
RA   Cheng N.-H., Pittman J.K., Shigaki T., Hirschi K.D.;
RT   "Characterization of CAX4, an Arabidopsis H(+)/cation antiporter.";
RL   Plant Physiol. 128:1245-1254(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Portal (Araport);
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA   Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA   Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA   Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A.,
RA   Guerinot M.L.;
RT   "Phylogenetic relationships within cation transporter families of
RT   Arabidopsis.";
RL   Plant Physiol. 126:1646-1667(2001).
RN   [5]
RP   BIOTECHNOLOGY.
RX   PubMed=16244156; DOI=10.1104/pp.105.066266;
RA   Park S., Cheng N.-H., Pittman J.K., Yoo K.S., Park J., Smith R.H.,
RA   Hirschi K.D.;
RT   "Increased calcium levels and prolonged shelf life in tomatoes
RT   expressing Arabidopsis H+/Ca2+ transporters.";
RL   Plant Physiol. 139:1194-1206(2005).
RN   [6]
RP   FUNCTION.
RX   PubMed=16845524; DOI=10.1007/s00425-006-0352-7;
RA   Koren'kov V., Park S., Cheng N.-H., Sreevidya C., Lachmansingh J.,
RA   Morris J., Hirschi K., Wagner G.J.;
RT   "Enhanced Cd(2+)-selective root-tonoplast-transport in tobaccos
RT   expressing Arabidopsis cation exchangers.";
RL   Planta 225:403-411(2007).
CC   -!- FUNCTION: Vacuolar cation/proton exchanger (CAX). Translocates
CC       Ca(2+) and other metal ions into vacuoles using the proton
CC       gradient formed by H(+)-ATPase and H(+)-pyrophosphatase. Cation
CC       selectivity transport in tobacco root tonoplast vesicles is
CC       Cd(2+)>Zn(2+)>>Ca(2+)>>>Mn(2+). {ECO:0000269|PubMed:16845524}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane
CC       {ECO:0000305|PubMed:11950973}; Multi-pass membrane protein
CC       {ECO:0000305|PubMed:11950973}. Note=Tonoplast.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q945S5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q945S5-2; Sequence=VSP_042221;
CC         Note=Derived from EST data. No experimental confirmation
CC         available.;
CC   -!- TISSUE SPECIFICITY: Expressed at low levels in all tissues.
CC       {ECO:0000269|PubMed:11950973}.
CC   -!- INDUCTION: Slightly induced by Mn(2+), Na(+) and Ni(2+).
CC       {ECO:0000269|PubMed:11950973}.
CC   -!- BIOTECHNOLOGY: CAX4 expression in tomato increases calcium content
CC       and prolonges shelf life and may serve as an alternative to the
CC       application of CaCl(2) used to extend shelf life in numerous
CC       agricultural commodities. {ECO:0000269|PubMed:16244156}.
CC   -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA)
CC       (TC 2.A.19) family. Cation/proton exchanger (CAX) subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB82265.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AF409107; AAK97656.1; -; mRNA.
DR   EMBL; AL161946; CAB82265.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED90352.1; -; Genomic_DNA.
DR   EMBL; CP002688; ANM69424.1; -; Genomic_DNA.
DR   PIR; T48170; T48170.
DR   RefSeq; NP_001331104.1; NM_001342594.1. [Q945S5-2]
DR   RefSeq; NP_568091.2; NM_120227.4. [Q945S5-2]
DR   UniGene; At.4687; -.
DR   ProteinModelPortal; Q945S5; -.
DR   STRING; 3702.AT5G01490.1; -.
DR   TCDB; 2.A.19.2.9; the ca(2+):cation antiporter (caca) family.
DR   iPTMnet; Q945S5; -.
DR   PaxDb; Q945S5; -.
DR   EnsemblPlants; AT5G01490.1; AT5G01490.1; AT5G01490. [Q945S5-2]
DR   EnsemblPlants; AT5G01490.2; AT5G01490.2; AT5G01490. [Q945S5-2]
DR   GeneID; 831754; -.
DR   Gramene; AT5G01490.1; AT5G01490.1; AT5G01490.
DR   Gramene; AT5G01490.2; AT5G01490.2; AT5G01490.
DR   KEGG; ath:AT5G01490; -.
DR   Araport; AT5G01490; -.
DR   TAIR; locus:2149720; AT5G01490.
DR   eggNOG; KOG1397; Eukaryota.
DR   eggNOG; COG0387; LUCA.
DR   HOGENOM; HOG000083011; -.
DR   InParanoid; Q945S5; -.
DR   KO; K07300; -.
DR   OMA; GTSNYMK; -.
DR   OrthoDB; EOG09360BTK; -.
DR   PhylomeDB; Q945S5; -.
DR   PRO; PR:Q945S5; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   Genevisible; Q945S5; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009705; C:plant-type vacuole membrane; IDA:TAIR.
DR   GO; GO:0015368; F:calcium:cation antiporter activity; IDA:TAIR.
DR   GO; GO:0015369; F:calcium:proton antiporter activity; IMP:TAIR.
DR   GO; GO:0009733; P:response to auxin; IMP:TAIR.
DR   GO; GO:0048364; P:root development; IMP:TAIR.
DR   InterPro; IPR004713; CaH_exchang.
DR   InterPro; IPR004798; CAX.
DR   InterPro; IPR004837; NaCa_Exmemb.
DR   Pfam; PF01699; Na_Ca_ex; 2.
DR   TIGRFAMs; TIGR00846; caca2; 1.
DR   TIGRFAMs; TIGR00378; cax; 1.
PE   1: Evidence at protein level;
DR   PRODOM; Q945S5.
DR   SWISS-2DPAGE; Q945S5.
KW   Alternative splicing; Antiport; Calcium; Calcium transport;
KW   Complete proteome; Ion transport; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport; Vacuole.
FT   CHAIN         1    446       Vacuolar cation/proton exchanger 4.
FT                                /FTId=PRO_0000270153.
FT   TOPO_DOM      1     69       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM     70     90       Helical. {ECO:0000255}.
FT   TOPO_DOM     91     96       Extracellular. {ECO:0000255}.
FT   TRANSMEM     97    117       Helical. {ECO:0000255}.
FT   TOPO_DOM    118    128       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    129    149       Helical. {ECO:0000255}.
FT   TOPO_DOM    150    162       Extracellular. {ECO:0000255}.
FT   TRANSMEM    163    183       Helical. {ECO:0000255}.
FT   TOPO_DOM    184    196       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    197    217       Helical. {ECO:0000255}.
FT   TOPO_DOM    218    238       Extracellular. {ECO:0000255}.
FT   TRANSMEM    239    259       Helical. {ECO:0000255}.
FT   TOPO_DOM    260    285       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    286    306       Helical. {ECO:0000255}.
FT   TOPO_DOM    307    319       Extracellular. {ECO:0000255}.
FT   TRANSMEM    320    340       Helical. {ECO:0000255}.
FT   TOPO_DOM    341    359       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    360    380       Helical. {ECO:0000255}.
FT   TOPO_DOM    381    388       Extracellular. {ECO:0000255}.
FT   TRANSMEM    389    409       Helical. {ECO:0000255}.
FT   TOPO_DOM    410    416       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    417    437       Helical. {ECO:0000255}.
FT   TOPO_DOM    438    446       Extracellular. {ECO:0000255}.
FT   REGION      138    173       Cation selection. {ECO:0000255}.
FT   REGION      337    372       Cation selection. {ECO:0000255}.
FT   VAR_SEQ     442    446       SKLLF -> TETNTTNHTITKR (in isoform 2).
FT                                {ECO:0000305}.
FT                                /FTId=VSP_042221.
FT   MUTAGEN      88     96       VAHRYDCPR->ICTYCGVSQ: Facilitates
FT                                Ca(2+)/H(+) exchange activity.
FT                                {ECO:0000269|PubMed:11950973}.
FT   CONFLICT     13     13       S -> C (in Ref. 1; AAK97656).
FT                                {ECO:0000305}.
FT   CONFLICT    273    273       H -> Y (in Ref. 1; AAK97656).
FT                                {ECO:0000305}.
FT   CONFLICT    399    404       FALSIL -> LAMSII (in Ref. 1; AAK97656).
FT                                {ECO:0000305}.
FT   CONFLICT    428    428       V -> F (in Ref. 1; AAK97656).
FT                                {ECO:0000305}.
SQ   SEQUENCE   446 AA;  48699 MW;  C8C69E511CC37B5B CRC64;
     MSSISTESSS NLSLLENGGG GSDKPTAETS RRVRRTVSAS SLIRKRSDLK LISRVRWEFM
     RRILTNLQEV LLGTKLFILF PAVPLAVVAH RYDCPRAWVF ALSLLGLTPL AERISFLTEQ
     IAFHTGPTVG GLMNATCGNA TEMIIAILAV GQRKMRIVKL SLLGSILSNL LFVLGTSLFL
     GGISNLRKHQ SFDPRQGDMN SMLLYLALLC QTLPMIMRFT MEAEEYDGSD VVVLSRASSF
     VMLIAYLAFL IFHLFSSHLS PPPPPLPQRE DVHDDDVSDK EEEGAVIGMW SAIFWLIIMT
     LLVALLSDYL VSTIQDAADS WGLSVGFIGI ILLPIVGNAA EHAGAVIFAF RNKLDITLGI
     ALGSATQIAL FVVPVTVLVA WTMGIEMDLN FNLLETACFA LSILVTSLVL QDGTSNYMKG
     LVLLLCYVVI AACFFVSNSP SSKLLF
//

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