(data stored in ACNUC13479 zone)

SWISSPROT: TAAC_ARATH

ID   TAAC_ARATH              Reviewed;         415 AA.
AC   Q9M024; Q8LCT8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   07-JUN-2017, entry version 113.
DE   RecName: Full=Thylakoid ADP,ATP carrier protein, chloroplastic;
DE   AltName: Full=Thylakoid ADP/ATP translocase;
DE   Flags: Precursor;
GN   Name=TAAC; OrderedLocusNames=At5g01500; ORFNames=F7A7.20;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Portal (Araport);
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY.
RX   PubMed=15003237; DOI=10.1016/j.tplants.2004.01.007;
RA   Picault N., Hodges M., Palmieri L., Palmieri F.;
RT   "The growing family of mitochondrial carriers in Arabidopsis.";
RL   Trends Plant Sci. 9:138-146(2004).
RN   [6]
RP   IDENTIFICATION, FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE,
RP   TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ENZYME REGULATION, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=17261580; DOI=10.1074/jbc.M609130200;
RA   Thuswaldner S., Lagerstedt J.O., Rojas-Stuetz M., Bouhidel K., Der C.,
RA   Leborgne-Castel N., Mishra A., Marty F., Schoefs B., Adamska I.,
RA   Persson B.L., Spetea C.;
RT   "Identification, expression, and functional analyses of a thylakoid
RT   ATP/ADP carrier from Arabidopsis.";
RL   J. Biol. Chem. 282:8848-8859(2007).
CC   -!- FUNCTION: Specifically transports adenine nucleotides. Involved in
CC       the uptake of ATP into thylakoids in exchange for lumenal ADP.
CC       {ECO:0000269|PubMed:17261580}.
CC   -!- ENZYME REGULATION: KM and Vmax values toward ATP only are
CC       increased by m-chlorocarbonyl cyanide phenylhydrazone (CCCP). The
CC       corresponding values for ADP are not affected.
CC       {ECO:0000269|PubMed:17261580}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=47 uM for ATP (for the recombinant protein)
CC         {ECO:0000269|PubMed:17261580};
CC         KM=0.5 uM for ATP (in vivo) {ECO:0000269|PubMed:17261580};
CC         KM=45 uM for ADP (for the recombinant protein)
CC         {ECO:0000269|PubMed:17261580};
CC         Vmax=0.71 nmol/h/mg enzyme toward ATP (for the recombinant
CC         protein) {ECO:0000269|PubMed:17261580};
CC         Vmax=0.53 nmol/h/mg enzyme toward ADP (for the recombinant
CC         protein) {ECO:0000269|PubMed:17261580};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000269|PubMed:17261580}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:17261580}. Plastid, chloroplast envelope
CC       {ECO:0000269|PubMed:17261580}. Note=Detected only in low amounts
CC       in the chloroplast envelope and in non-photosynthetic plastids.
CC   -!- TISSUE SPECIFICITY: Highly expressed in developing photosynthetic
CC       organs such as leaves, flower buds and green siliques. Also
CC       detected in roots, flowers, mature leaves and stems.
CC       {ECO:0000269|PubMed:17261580}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in dark-grown seedlings and remains
CC       stable throughout the greening process. Highest expression in
CC       developing green tissues and in leaves undergoing senescence or
CC       abiotic stress, with the exception of heat shock conditions that
CC       induced a drastic reduction of expression.
CC       {ECO:0000269|PubMed:17261580}.
CC   -!- DISRUPTION PHENOTYPE: Plants show a 30-40% reduction in the
CC       thylakoid ATP transport and metabolism.
CC       {ECO:0000269|PubMed:17261580}.
CC   -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29)
CC       family. {ECO:0000305}.
DR   EMBL; AL161946; CAB82266.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED90353.1; -; Genomic_DNA.
DR   EMBL; AY074566; AAL67106.1; -; mRNA.
DR   EMBL; BT006336; AAP21144.1; -; mRNA.
DR   EMBL; AY086408; AAM64475.1; -; mRNA.
DR   PIR; T48171; T48171.
DR   RefSeq; NP_195770.1; NM_120228.4.
DR   UniGene; At.43427; -.
DR   ProteinModelPortal; Q9M024; -.
DR   SMR; Q9M024; -.
DR   STRING; 3702.AT5G01500.1; -.
DR   TCDB; 2.A.29.23.3; the mitochondrial carrier (mc) family.
DR   PaxDb; Q9M024; -.
DR   EnsemblPlants; AT5G01500.1; AT5G01500.1; AT5G01500.
DR   GeneID; 831861; -.
DR   Gramene; AT5G01500.1; AT5G01500.1; AT5G01500.
DR   KEGG; ath:AT5G01500; -.
DR   Araport; AT5G01500; -.
DR   TAIR; locus:2149725; AT5G01500.
DR   eggNOG; KOG0752; Eukaryota.
DR   eggNOG; ENOG410ZRF1; LUCA.
DR   HOGENOM; HOG000165726; -.
DR   InParanoid; Q9M024; -.
DR   KO; K14684; -.
DR   OMA; CKQVCLY; -.
DR   OrthoDB; EOG09360EFC; -.
DR   PhylomeDB; Q9M024; -.
DR   PRO; PR:Q9M024; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   Genevisible; Q9M024; AT.
DR   GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR   GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR   GO; GO:0009536; C:plastid; IDA:TAIR.
DR   GO; GO:0009526; C:plastid envelope; IDA:TAIR.
DR   GO; GO:0042651; C:thylakoid membrane; IDA:TAIR.
DR   GO; GO:0005347; F:ATP transmembrane transporter activity; IDA:TAIR.
DR   GO; GO:0006839; P:mitochondrial transport; IBA:GO_Central.
DR   GO; GO:0010117; P:photoprotection; IMP:TAIR.
DR   GO; GO:0010206; P:photosystem II repair; IMP:TAIR.
DR   Gene3D; 1.50.40.10; -; 1.
DR   InterPro; IPR002067; Mit_carrier.
DR   InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR   InterPro; IPR023395; Mt_carrier_dom.
DR   Pfam; PF00153; Mito_carr; 3.
DR   PRINTS; PR00926; MITOCARRIER.
DR   SUPFAM; SSF103506; SSF103506; 1.
DR   PROSITE; PS50920; SOLCAR; 3.
PE   1: Evidence at protein level;
DR   PRODOM; Q9M024.
DR   SWISS-2DPAGE; Q9M024.
KW   Chloroplast; Complete proteome; Membrane; Plastid; Reference proteome;
KW   Repeat; Thylakoid; Transit peptide; Transmembrane;
KW   Transmembrane helix; Transport.
FT   TRANSIT       1     61       Chloroplast. {ECO:0000255}.
FT   CHAIN        62    415       Thylakoid ADP,ATP carrier protein,
FT                                chloroplastic.
FT                                /FTId=PRO_0000313084.
FT   TRANSMEM    106    126       Helical; Name=1. {ECO:0000250}.
FT   TRANSMEM    182    207       Helical; Name=2. {ECO:0000250}.
FT   TRANSMEM    219    239       Helical; Name=3. {ECO:0000250}.
FT   TRANSMEM    273    293       Helical; Name=4. {ECO:0000250}.
FT   TRANSMEM    309    329       Helical; Name=5. {ECO:0000255}.
FT   TRANSMEM    362    388       Helical; Name=6. {ECO:0000250}.
FT   REPEAT      113    205       Solcar 1.
FT   REPEAT      213    296       Solcar 2.
FT   REPEAT      307    387       Solcar 3.
FT   COMPBIAS     74     78       Poly-Gly.
FT   BINDING     187    187       Nucleotide. {ECO:0000250}.
FT   CONFLICT    411    411       I -> T (in Ref. 4; AAM64475).
FT                                {ECO:0000305}.
SQ   SEQUENCE   415 AA;  45090 MW;  D32B575A04994C2B CRC64;
     MGEEKSLLQF RSFPSLKTSD FALTEEPSWR LENNVSSNRR RGNKRSGGVF TNFASLSVAI
     RRDRRESTFN GRNGGGGGAF ASVSVVIPKE EDEFAPTSAQ LLKNPIALLS IVPKDAALFF
     AGAFAGAAAK SVTAPLDRIK LLMQTHGVRA GQQSAKKAIG FIEAITLIGK EEGIKGYWKG
     NLPQVIRIVP YSAVQLFAYE TYKKLFRGKD GQLSVLGRLG AGACAGMTST LITYPLDVLR
     LRLAVEPGYR TMSQVALNML REEGVASFYN GLGPSLLSIA PYIAINFCVF DLVKKSLPEK
     YQQKTQSSLL TAVVAAAIAT GTCYPLDTIR RQMQLKGTPY KSVLDAFSGI IAREGVVGLY
     RGFVPNALKS MPNSSIKLTT FDIVKKLIAA SEKEIQRIAD DNRKKASPNT IDEQT
//

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