(data stored in ACNUC13479 zone)

SWISSPROT: LRK64_ARATH

ID   LRK64_ARATH             Reviewed;         691 AA.
AC   Q66GN2; Q9M019;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   08-MAY-2019, entry version 107.
DE   RecName: Full=Lectin-domain containing receptor kinase VI.4;
DE            Short=LecRK-VI.4;
DE            EC=2.7.11.1;
DE   AltName: Full=Lectin receptor kinase A4.3;
DE   Flags: Precursor;
GN   Name=LECRK64; Synonyms=LECRKA4.3; OrderedLocusNames=At5g01560;
GN   ORFNames=F7A7.80;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana
RT   reference genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY.
RX   DOI=10.1080/0735-260291044287;
RA   Barre A., Herve C., Lescure B., Rouge P.;
RT   "Lectin receptor kinases in plants.";
RL   Crit. Rev. Plant Sci. 21:379-399(2002).
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19773388; DOI=10.1093/jxb/erp277;
RA   Bouwmeester K., Govers F.;
RT   "Arabidopsis L-type lectin receptor kinases: phylogeny,
RT   classification, and expression profiles.";
RL   J. Exp. Bot. 60:4383-4396(2009).
RN   [6]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=18987212; DOI=10.1104/pp.108.130583;
RA   Xin Z., Wang A., Yang G., Gao P., Zheng Z.-L.;
RT   "The Arabidopsis a4 subfamily of lectin receptor kinases negatively
RT   regulates abscisic acid response in seed germination.";
RL   Plant Physiol. 149:434-444(2009).
CC   -!- FUNCTION: Involved in negative regulation of abscisic acid
CC       response in seed germination. {ECO:0000269|PubMed:18987212}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass
CC       type I membrane protein {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Slight enhancement in abscisic acid-
CC       inhibited germination. Redundant with LECRKA4.1 and LECRKA4.2.
CC       {ECO:0000269|PubMed:18987212}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the protein
CC       kinase superfamily. Ser/Thr protein kinase family. {ECO:0000305}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the leguminous
CC       lectin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB82272.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
CC   -!- WEB RESOURCE: Name=PlantP kinase Classification PPC;
CC       URL="http://plantsp.genomics.purdue.edu/family/class.html";
DR   EMBL; AL161946; CAB82272.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002688; AED90360.1; -; Genomic_DNA.
DR   EMBL; BT015370; AAU05493.1; -; mRNA.
DR   PIR; T48177; T48177.
DR   RefSeq; NP_195776.2; NM_120234.4.
DR   SMR; Q66GN2; -.
DR   STRING; 3702.AT5G01560.1; -.
DR   iPTMnet; Q66GN2; -.
DR   PaxDb; Q66GN2; -.
DR   PRIDE; Q66GN2; -.
DR   EnsemblPlants; AT5G01560.1; AT5G01560.1; AT5G01560.
DR   GeneID; 830345; -.
DR   Gramene; AT5G01560.1; AT5G01560.1; AT5G01560.
DR   KEGG; ath:AT5G01560; -.
DR   Araport; AT5G01560; -.
DR   TAIR; locus:2149810; AT5G01560.
DR   eggNOG; ENOG410IKU3; Eukaryota.
DR   eggNOG; COG0515; LUCA.
DR   HOGENOM; HOG000116555; -.
DR   InParanoid; Q66GN2; -.
DR   OMA; PRTPMIS; -.
DR   OrthoDB; 684563at2759; -.
DR   PhylomeDB; Q66GN2; -.
DR   PRO; PR:Q66GN2; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q66GN2; baseline and differential.
DR   Genevisible; Q66GN2; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR   GO; GO:0004675; F:transmembrane receptor protein serine/threonine kinase activity; IBA:GO_Central.
DR   GO; GO:0009738; P:abscisic acid-activated signaling pathway; IMP:TAIR.
DR   GO; GO:0006952; P:defense response; IBA:GO_Central.
DR   GO; GO:0042742; P:defense response to bacterium; IBA:GO_Central.
DR   GO; GO:0002229; P:defense response to oomycetes; IBA:GO_Central.
DR   GO; GO:0009845; P:seed germination; IMP:TAIR.
DR   CDD; cd06899; lectin_legume_LecRK_Arcelin_Co; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR001220; Legume_lectin_dom.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   Pfam; PF00139; Lectin_legB; 1.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   2: Evidence at transcript level;
DR   PRODOM; Q66GN2.
DR   SWISS-2DPAGE; Q66GN2.
KW   ATP-binding; Cell membrane; Complete proteome; Kinase; Lectin;
KW   Membrane; Nucleotide-binding; Receptor; Reference proteome;
KW   Serine/threonine-protein kinase; Signal; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL        1     19       {ECO:0000255}.
FT   CHAIN        20    691       Lectin-domain containing receptor kinase
FT                                VI.4.
FT                                /FTId=PRO_0000364131.
FT   TOPO_DOM     20    306       Extracellular. {ECO:0000255}.
FT   TRANSMEM    307    327       Helical. {ECO:0000255}.
FT   TOPO_DOM    328    691       Cytoplasmic. {ECO:0000255}.
FT   DOMAIN      363    641       Protein kinase. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00159}.
FT   NP_BIND     369    377       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00159}.
FT   REGION       26    273       Legume-lectin like.
FT   COMPBIAS    290    294       Poly-Pro.
FT   COMPBIAS    648    689       Ser-rich.
FT   ACT_SITE    491    491       Proton acceptor. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00159, ECO:0000255|PROSITE-
FT                                ProRule:PRU10027}.
FT   BINDING     392    392       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00159}.
SQ   SEQUENCE   691 AA;  76735 MW;  B5CBCEDC8ACEE226 CRC64;
     MGRAKSMVSL LLVLFLVRAH VATTETTTEF IFHGFKGNQS EIHMQGDSTI TSNGLLRLTD
     RNSDVVGTAF YHKPVRLLDS NSTNTTVRSF STSFIFIIPS SSTSNGGFGF TFTLSPTPNR
     TDADPEQYMG LLNERNDGNS SNHVFAVEFD TVQGFKDGTN RIGNHIGLNF NSLSSDVQEP
     VAYFNNNDSQ KEEFQLVSGE PIQVFLDYHG PTKTLNLTVY PTRLGYKPRI PLISREVPKL
     SDIVVDEMFV GFTAATGRHG QSSAHYVMGW SFASGGEHPL AAMLDISQLP PPPPNKAKKR
     GYNGKVIALI VALSTVISIM LVLLFLFMMY KKRMQQEEIL EDWEIDHPHR FRYRDLYKAT
     EGFKENRVVG TGGFGIVYRG NIRSSSDQIA VKKITPNSMQ GVREFVAEIE SLGRLRHKNL
     VNLQGWCKHR NDLLLIYDYI PNGSLDSLLY SKPRRSGAVL SWNARFQIAK GIASGLLYLH
     EEWEQIVIHR DVKPSNVLID SDMNPRLGDF GLARLYERGS QSCTTVVVGT IGYMAPELAR
     NGNSSSASDV FAFGVLLLEI VSGRKPTDSG TFFIADWVME LQASGEILSA IDPRLGSGYD
     EGEARLALAV GLLCCHHKPE SRPLMRMVLR YLNRDEDVPE IHDNWGYSDS SRTDLGSKLV
     GYISSDRASS SHSHTSSSLT RISSTSLISG R
//

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