(data stored in ACNUC13479 zone)

SWISSPROT: TIC56_ARATH

ID   TIC56_ARATH             Reviewed;         527 AA.
AC   Q7Y1W1; Q7Y1Z4; Q9M016;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   30-AUG-2017, entry version 77.
DE   RecName: Full=Protein TIC 56, chloroplastic {ECO:0000303|PubMed:23372012};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 56 {ECO:0000303|PubMed:23372012};
DE            Short=AtTIC56 {ECO:0000303|PubMed:23372012};
DE   Flags: Precursor;
GN   Name=TIC56 {ECO:0000303|PubMed:23372012};
GN   OrderedLocusNames=At5g01590 {ECO:0000312|Araport:AT5G01590};
GN   ORFNames=F7A7_110 {ECO:0000312|EMBL:CAB82275.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000312|EMBL:AAP41935.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Portal (Araport);
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   FUNCTION, COMPONENT OF THE 1-MD COMPLEX, SUBUNIT, IDENTIFICATION BY
RP   MASS SPECTROMETRY, DEAMIDATION AT ASN-350, SUBCELLULAR LOCATION, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=23372012; DOI=10.1126/science.1229262;
RA   Kikuchi S., Bedard J., Hirano M., Hirabayashi Y., Oishi M., Imai M.,
RA   Takase M., Ide T., Nakai M.;
RT   "Uncovering the protein translocon at the chloroplast inner envelope
RT   membrane.";
RL   Science 339:571-574(2013).
CC   -!- FUNCTION: Involved in protein precursor import into chloroplasts.
CC       May be part of an intermediate translocation complex acting as a
CC       protein-conducting channel at the inner envelope.
CC       {ECO:0000269|PubMed:23372012}.
CC   -!- SUBUNIT: Part of the Tic complex. Component of the 1-MD complex,
CC       composed of TIC20-I, TIC214, TIC100 and TIC56. Interacts with the
CC       translocating preproteins. Hydrolysis of ATP is essential for the
CC       formation of this complex (PubMed:23372012). The 1-MD complex
CC       interacts with TIC21 (By similarity).
CC       {ECO:0000250|UniProtKB:Q8GZ79, ECO:0000269|PubMed:23372012}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000269|PubMed:23372012}.
CC   -!- DISRUPTION PHENOTYPE: Severe defects during embryogenesis,
CC       producing abnormal embryos and thereby leading to a lethality of
CC       young seedlings. {ECO:0000269|PubMed:23372012}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB82275.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AL161946; CAB82275.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED90363.1; -; Genomic_DNA.
DR   EMBL; AY050767; AAP41935.1; -; mRNA.
DR   EMBL; BT008696; AAP40502.1; -; mRNA.
DR   PIR; T48180; T48180.
DR   RefSeq; NP_568092.4; NM_120237.6.
DR   UniGene; At.4694; -.
DR   UniGene; At.68146; -.
DR   ProteinModelPortal; Q7Y1W1; -.
DR   STRING; 3702.AT5G01590.1; -.
DR   TCDB; 3.A.9.1.2; the chloroplast envelope protein translocase (cept or tic-toc) family.
DR   iPTMnet; Q7Y1W1; -.
DR   PaxDb; Q7Y1W1; -.
DR   EnsemblPlants; AT5G01590.1; AT5G01590.1; AT5G01590.
DR   GeneID; 830369; -.
DR   Gramene; AT5G01590.1; AT5G01590.1; AT5G01590.
DR   KEGG; ath:AT5G01590; -.
DR   Araport; AT5G01590; -.
DR   TAIR; locus:2149735; AT5G01590.
DR   eggNOG; ENOG410IHJJ; Eukaryota.
DR   eggNOG; ENOG4111F7B; LUCA.
DR   HOGENOM; HOG000006363; -.
DR   InParanoid; Q7Y1W1; -.
DR   OMA; RGPMELI; -.
DR   OrthoDB; EOG093606GV; -.
DR   PhylomeDB; Q7Y1W1; -.
DR   PRO; PR:Q7Y1W1; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q7Y1W1; baseline and differential.
DR   Genevisible; Q7Y1W1; AT.
DR   GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR   GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR   GO; GO:0009706; C:chloroplast inner membrane; IDA:TAIR.
DR   GO; GO:0009536; C:plastid; IDA:TAIR.
DR   GO; GO:0008565; F:protein transporter activity; IDA:TAIR.
DR   GO; GO:0045037; P:protein import into chloroplast stroma; IDA:TAIR.
DR   Gene3D; 3.30.1490.40; -; 1.
DR   InterPro; IPR025640; DUF4339.
DR   InterPro; IPR035445; GYF-like_domain.
DR   Pfam; PF14237; DUF4339; 1.
PE   1: Evidence at protein level;
DR   PRODOM; Q7Y1W1.
DR   SWISS-2DPAGE; Q7Y1W1.
KW   Chloroplast; Complete proteome; Membrane; Plastid;
KW   Plastid inner membrane; Protein transport; Reference proteome;
KW   Transit peptide; Transport.
FT   TRANSIT       1     48       Chloroplast. {ECO:0000255}.
FT   CHAIN        49    527       Protein TIC 56, chloroplastic.
FT                                /FTId=PRO_0000431666.
FT   COMPBIAS    510    518       Poly-Glu. {ECO:0000255}.
FT   MOD_RES     350    350       Deamidated asparagine.
FT                                {ECO:0000269|PubMed:23372012}.
FT   CONFLICT    259    259       T -> K (in Ref. 3; AAP40502).
FT                                {ECO:0000305}.
FT   CONFLICT    414    414       M -> V (in Ref. 3; AAP40502).
FT                                {ECO:0000305}.
SQ   SEQUENCE   527 AA;  61625 MW;  2288A8B688D0E79E CRC64;
     MSSMNFNPFQ NWFEKPPNPV PSINFVSLAD SFFPKSQSPN FASIGLPKFS KKSPKPETAG
     TDEPGPYKQI AEQFLWECEN IPDYRHTPEV DKLLNEDPVF EKKENPSTEE IEAEQKWWES
     FRASPVVQFM TRAEEIADDM NKMELEDNDT PYRKEDKDYW RAIPHVPGFD GRPMPRKAIK
     SKEESDDKFW DFMKQFLFGL WGFRQRPYPP GRPIDVAQAI GYKRLEKRYY DFIMKTGGWW
     YKDRLGRSRG PCEIITLKTA YGAGIIDRDT FIWGEDMDEW APIHMVYGLE PAIATWEVRL
     GAAATAFLHK LQKGIPPWVP LKGREPKTYK QLQKEAIESK KRDMAVLEAN GGVWPGVRTP
     SHALFLWASG SELTTVLESD HMPNKFIPKQ LRLELAKVIP GLRPWEVISI EQAMDQISYG
     GEWYREPLGT YTTGPPYIRE WNRSVMRLFR IFYNLSVRVG QKLERTVPGF DTIMDKVQKD
     YDKRIARRMK RREEELREED LKHYSGRTDE DEEEEEEEDD DSNSKKD
//

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