(data stored in ACNUC13479 zone)

SWISSPROT: P2C65_ARATH

ID   P2C65_ARATH             Reviewed;         382 AA.
AC   Q6NKS1; Q2V3A9; Q9M006;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   30-AUG-2017, entry version 96.
DE   RecName: Full=Probable protein phosphatase 2C 65;
DE            Short=AtPP2C65;
DE            EC=3.1.3.16;
GN   OrderedLocusNames=At5g01700; ORFNames=F7A7.220;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Portal (Araport);
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RX   PubMed=14993207; DOI=10.1101/gr.1515604;
RA   Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA   Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G.,
RA   Caboche M., Weissenbach J., Salanoubat M.;
RT   "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT   combined approach to evaluate and improve Arabidopsis genome
RT   annotation.";
RL   Genome Res. 14:406-413(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=cv. Columbia;
RA   Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA   Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C.,
RA   Zhong Y.;
RT   "Genome-wide and expression analysis of protein phosphatase 2C in rice
RT   and Arabidopsis.";
RL   BMC Genomics 9:550-550(2008).
CC   -!- CATALYTIC ACTIVITY: [a protein]-serine/threonine phosphate + H(2)O
CC       = [a protein]-serine/threonine + phosphate.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium or manganese ions per subunit.
CC       {ECO:0000250};
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q6NKS1-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6NKS1-2; Sequence=VSP_036776;
CC         Note=No experimental confirmation available.;
CC   -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BX831823; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC       Sequence=CAB82286.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AL161946; CAB82286.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED90378.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED90379.1; -; Genomic_DNA.
DR   EMBL; BX831823; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BT011618; AAS47624.1; -; mRNA.
DR   EMBL; BT012622; AAT06441.1; -; mRNA.
DR   PIR; T48191; T48191.
DR   RefSeq; NP_001031819.1; NM_001036742.2. [Q6NKS1-1]
DR   RefSeq; NP_195790.4; NM_120248.5. [Q6NKS1-2]
DR   UniGene; At.33516; -.
DR   ProteinModelPortal; Q6NKS1; -.
DR   SMR; Q6NKS1; -.
DR   STRING; 3702.AT5G01700.2; -.
DR   PaxDb; Q6NKS1; -.
DR   EnsemblPlants; AT5G01700.1; AT5G01700.1; AT5G01700. [Q6NKS1-2]
DR   EnsemblPlants; AT5G01700.2; AT5G01700.2; AT5G01700. [Q6NKS1-1]
DR   GeneID; 831695; -.
DR   Gramene; AT5G01700.1; AT5G01700.1; AT5G01700.
DR   Gramene; AT5G01700.2; AT5G01700.2; AT5G01700.
DR   KEGG; ath:AT5G01700; -.
DR   Araport; AT5G01700; -.
DR   TAIR; locus:2149775; AT5G01700.
DR   eggNOG; KOG0698; Eukaryota.
DR   eggNOG; COG0631; LUCA.
DR   HOGENOM; HOG000233897; -.
DR   InParanoid; Q6NKS1; -.
DR   OMA; ASWEGCF; -.
DR   OrthoDB; EOG09360B91; -.
DR   PhylomeDB; Q6NKS1; -.
DR   Reactome; R-ATH-70895; Branched-chain amino acid catabolism.
DR   PRO; PR:Q6NKS1; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   Genevisible; Q6NKS1; AT.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004722; F:protein serine/threonine phosphatase activity; IEA:InterPro.
DR   Gene3D; 3.60.40.10; -; 1.
DR   InterPro; IPR015655; PP2C.
DR   InterPro; IPR001932; PPM-type_phosphatase_dom.
DR   PANTHER; PTHR13832; PTHR13832; 1.
DR   Pfam; PF00481; PP2C; 1.
DR   SMART; SM00332; PP2Cc; 1.
DR   SUPFAM; SSF81606; SSF81606; 1.
DR   PROSITE; PS51746; PPM_2; 1.
PE   2: Evidence at transcript level;
DR   PRODOM; Q6NKS1.
DR   SWISS-2DPAGE; Q6NKS1.
KW   Alternative splicing; Complete proteome; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Protein phosphatase; Reference proteome.
FT   CHAIN         1    382       Probable protein phosphatase 2C 65.
FT                                /FTId=PRO_0000367987.
FT   DOMAIN       47    337       PPM-type phosphatase.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01082}.
FT   METAL        83     83       Manganese 1. {ECO:0000250}.
FT   METAL        83     83       Manganese 2. {ECO:0000250}.
FT   METAL        84     84       Manganese 1; via carbonyl oxygen.
FT                                {ECO:0000250}.
FT   METAL       282    282       Manganese 2. {ECO:0000250}.
FT   METAL       328    328       Manganese 2. {ECO:0000250}.
FT   VAR_SEQ       1     49       Missing (in isoform 2).
FT                                {ECO:0000303|PubMed:14993207}.
FT                                /FTId=VSP_036776.
SQ   SEQUENCE   382 AA;  42004 MW;  27DA3A16DE5E77CA CRC64;
     MGVCCSKGTG IIVEHGADDG NECGDGEAEV RDTNDGAVVR TRGSSKHVSM SIKQGKKGIN
     QDAMTVWENF GGEEDTIFCG VFDGHGPMGH KISRHVCENL PSRVHSKIRS SKSAGDENIE
     NNSSQSQEEL FREFEDILVT FFKQIDSELG LDSPYDSFCS GTTAVTVFKQ ADCLVIANLG
     HSRAVLGTRS KNSFKAVQLT VDLKPCVQRE AERIVSCKGR VFAMEEEPDV YRVWMPDDDC
     PGLAMSRAFG DFCLKDYGLV CIPDVFCRKV SREDEFVVLA TDGIWDVLSN EEVVKVVGSC
     KDRSVAAEML VQRAARTWRT KFPASKADDC AVVVLYLNHR PYPREGNVSR AISTISWRSN
     KSNNECYGAA PLSPLGLSQR VS
//

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