(data stored in ACNUC13479 zone)

SWISSPROT: SCAR4_ARATH

ID   SCAR4_ARATH             Reviewed;        1170 AA.
AC   Q5XPJ6; Q9LCZ7;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   07-JUN-2017, entry version 81.
DE   RecName: Full=Protein SCAR4;
DE            Short=AtSCAR4;
DE   AltName: Full=Protein WAVE3;
GN   Name=SCAR4; OrderedLocusNames=At5g01730; ORFNames=F7A7.250;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=15534215; DOI=10.1073/pnas.0407392101;
RA   Frank M., Egile C., Dyachok J., Djakovic S., Nolasco M., Li R.,
RA   Smith L.G.;
RT   "Activation of Arp2/3 complex-dependent actin polymerization by plant
RT   proteins distantly related to Scar/WAVE.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:16379-16384(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Portal (Araport);
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION, AND IDENTIFICATION.
RX   PubMed=15316111; DOI=10.1105/tpc.104.023739;
RA   Brembu T., Winge P., Seem M., Bones A.M.;
RT   "NAPP and PIRP encode subunits of a putative wave regulatory protein
RT   complex involved in plant cell morphogenesis.";
RL   Plant Cell 16:2335-2349(2004).
RN   [5]
RP   INTERACTION WITH SPK1.
RX   PubMed=17267444; DOI=10.1242/dev.02792;
RA   Uhrig J.F., Mutondo M., Zimmermann I., Deeks M.J., Machesky L.M.,
RA   Thomas P., Uhrig S., Rambke C., Hussey P.J., Huelskamp M.;
RT   "The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell
RT   morphogenesis.";
RL   Development 134:967-977(2007).
CC   -!- FUNCTION: Involved in regulation of actin and microtubule
CC       organization. Part of a WAVE complex that activates the Arp2/3
CC       complex. Regulates trichome branch positioning and expansion.
CC       {ECO:0000269|PubMed:15316111}.
CC   -!- SUBUNIT: Interacts with SPK1. {ECO:0000269|PubMed:17267444}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC   -!- TISSUE SPECIFICITY: Expressed in expanding cotyledons, expanding
CC       leaves and expanding siliques containing developing embryos.
CC       Detected in unopened flower buds and in the expanding tip region
CC       of roots. Reduced expression in mature leaves.
CC       {ECO:0000269|PubMed:15534215}.
CC   -!- DOMAIN: Activates the Arp2/3 complex and binds actin through the
CC       C-terminal VCA (verprolin homology/cofilin homology/acidic) domain
CC       consisting of a WH2 domain followed by an Arp2/3-binding acidic
CC       motif (A), separated by a conserved linker region (C). Binds BRK1
CC       through the N-terminal Scar homology domain (SHD).
CC   -!- SIMILARITY: Belongs to the SCAR/WAVE family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB82289.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AY743927; AAU93852.1; -; mRNA.
DR   EMBL; AL161946; CAB82289.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED90383.1; -; Genomic_DNA.
DR   EMBL; CP002688; ANM68918.1; -; Genomic_DNA.
DR   PIR; T48194; T48194.
DR   RefSeq; NP_001318456.1; NM_001342607.1.
DR   RefSeq; NP_195793.3; NM_120251.4.
DR   UniGene; At.71562; -.
DR   ProteinModelPortal; Q5XPJ6; -.
DR   SMR; Q5XPJ6; -.
DR   BioGrid; 16963; 3.
DR   IntAct; Q5XPJ6; 3.
DR   STRING; 3702.AT5G01730.1; -.
DR   iPTMnet; Q5XPJ6; -.
DR   PaxDb; Q5XPJ6; -.
DR   PRIDE; Q5XPJ6; -.
DR   EnsemblPlants; AT5G01730.1; AT5G01730.1; AT5G01730.
DR   EnsemblPlants; AT5G01730.4; AT5G01730.4; AT5G01730.
DR   GeneID; 831687; -.
DR   Gramene; AT5G01730.1; AT5G01730.1; AT5G01730.
DR   Gramene; AT5G01730.4; AT5G01730.4; AT5G01730.
DR   KEGG; ath:AT5G01730; -.
DR   Araport; AT5G01730; -.
DR   TAIR; locus:2149820; AT5G01730.
DR   HOGENOM; HOG000154263; -.
DR   InParanoid; Q5XPJ6; -.
DR   OMA; DHSTFFY; -.
DR   OrthoDB; EOG093611RW; -.
DR   PhylomeDB; Q5XPJ6; -.
DR   PRO; PR:Q5XPJ6; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q5XPJ6; baseline and differential.
DR   Genevisible; Q5XPJ6; AT.
DR   GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0010287; C:plastoglobule; IDA:TAIR.
DR   GO; GO:0031209; C:SCAR complex; TAS:TAIR.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   GO; GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
DR   GO; GO:0051127; P:positive regulation of actin nucleation; IMP:TAIR.
DR   InterPro; IPR028288; SCAR/WAVE_fam.
DR   PANTHER; PTHR12902; PTHR12902; 1.
PE   1: Evidence at protein level;
DR   PRODOM; Q5XPJ6.
DR   SWISS-2DPAGE; Q5XPJ6.
KW   Actin-binding; Complete proteome; Cytoplasm; Cytoskeleton;
KW   Reference proteome.
FT   CHAIN         1   1170       Protein SCAR4.
FT                                /FTId=PRO_0000189007.
FT   DOMAIN     1105   1123       WH2.
FT   COMPBIAS    344    347       Poly-Ser.
FT   COMPBIAS   1035   1038       Poly-Glu.
SQ   SEQUENCE   1170 AA;  128618 MW;  96522221853ED25D CRC64;
     MALTRYQIRN EYGLADKELY QSADKEDPEA LLEAASMAGL VGVLRQLGDL SEFAAEVFHC
     LHEQLMTTAA RGHGLAMRLQ HLEADFPSVE IPILSQTDHS TFFYEPGLEW HSDLQTKEDL
     ISPRNLPRCI MDSYEECHGP PQLFLLDKFD VAGSGSCLKR YSDPSLLKTH TTSAVVATSK
     LGKDKRLRQS KKKGSHTTIK ETPEDSRTSH AKLHQLFFLE HVENGHRNPE FHVKLKRRQL
     NGPPINSSSG ASYMEKFLKN SSPYCERVHG TMDQSSPAME TEVTVCSEQE DLPIPSLVYS
     NSGGTRKYNE MEIESIAGHE ILEIPFVPHE ITVNEKSPVV CLESSSSVNL CCKTNNDADS
     PASTESEVKE AGSDDKAGCD HGFPGFGQPQ ICTNAEVNQT EVLTQFSNVL RHSPEEGESS
     LLCTDIQRAS PESKPHKAEE AAVDLDESFS QMTPDIDSAG MGTLEILQTP FSLSCYESPA
     NLPEDSGSHL ELQSNKANAE ACEVFEVRRD PMLNISPETH LLKVTQVPQD AYEGGTNDVH
     SQHVFSVETA SEISVSALVE DQFSSITNQE IEALESEDIS SEAGHFIPDT KKSLNETSVA
     LESDFLLPNH YISTFDNFED LSLSADAQDY AAPKEDETNS QDGSSMNPAQ SKHISTSEIS
     SENGTLMSDT PRDLHTGYGS LSASSCLEDG LANPDLAEIS SYSGQEDPQT MSIVSDDSSD
     PEVPIPDGTC FAGDVDHDNQ TGLNNKAIET VPQKELETIS DPQESLLGTE ECLSSEYCLQ
     IQNQRQESPS ETGSANSRTS SDESPPTQNG SVGVQSSPLD VFPSSITEIE ALHAPYQEIF
     TSLNDHISES VLSKGLTDEE DFLNVSPESI LPLSTSLHET PQANPEITPP LPPLPPTQWW
     MGKLVESTEM PSLAGSGNNS FNIQRDENTQ NGSVQANEAQ YPSEVSVTDG ENHNFHIYTE
     ESKATEEQSP SGVNGTSDTY MESKHKCLNR TPEDSFSLAE SAQGLEADWR TEAMALEWFS
     QNLREHNNPH PAKLEEEEPQ VDHPLEKPGQ TKFRQTLRDN NSYNQNQKAG KLKRDEDTLV
     IGIDRSMLRK VSEGNRTHVG ARVDENDSLL EIIRSKSFNL RPADASGRPN FQVAVPKTNL
     KVAAILEKAN TLRQAMAGSD DEHDSDSWSE
//

If you have problems or comments...

PBIL Back to PBIL home page