(data stored in ACNUC13479 zone)

SWISSPROT: OFP1_ARATH

ID   OFP1_ARATH              Reviewed;         270 AA.
AC   Q9LZW2;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   07-JUN-2017, entry version 89.
DE   RecName: Full=Transcription repressor OFP1;
DE   AltName: Full=Ovate family protein 1;
DE            Short=AtOFP1;
GN   Name=OFP1; OrderedLocusNames=At5g01840; ORFNames=T20L15.110;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Portal (Araport);
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, INTERACTION WITH ATH1; BLH1; BLH2; BLH3; BLH4; BLH5; BLH10;
RP   KNAT1; KNAT3; KNAT4; KNAT5 AND KNAT7, SUBCELLULAR LOCATION, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=15781858; DOI=10.1073/pnas.0501181102;
RA   Hackbusch J., Richter K., Muller J., Salamini F., Uhrig J.F.;
RT   "A central role of Arabidopsis thaliana ovate family proteins in
RT   networking and subcellular localization of 3-aa loop extension
RT   homeodomain proteins.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:4908-4912(2005).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=17461792; DOI=10.1111/j.1365-313X.2007.03096.x;
RA   Wang S., Chang Y., Guo J., Chen J.G.;
RT   "Arabidopsis Ovate Family Protein 1 is a transcriptional repressor
RT   that suppresses cell elongation.";
RL   Plant J. 50:858-872(2007).
RN   [5]
RP   FUNCTION, INTERACTION WITH KU70, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=20844935; DOI=10.1007/s11103-010-9685-5;
RA   Wang Y.K., Chang W.C., Liu P.F., Hsiao M.K., Lin C.T., Lin S.M.,
RA   Pan R.L.;
RT   "Ovate family protein 1 as a plant Ku70 interacting protein involving
RT   in DNA double-strand break repair.";
RL   Plant Mol. Biol. 74:453-466(2010).
RN   [6]
RP   FUNCTION, INTERACTION WITH KNAT7, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=21457372; DOI=10.1111/j.1365-313X.2011.04595.x;
RA   Li E., Wang S., Liu Y., Chen J.G., Douglas C.J.;
RT   "OVATE FAMILY PROTEIN4 (OFP4) interaction with KNAT7 regulates
RT   secondary cell wall formation in Arabidopsis thaliana.";
RL   Plant J. 67:328-341(2011).
RN   [7]
RP   FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, AND DISRUPTION PHENOTYPE.
RX   PubMed=21886836; DOI=10.1371/journal.pone.0023896;
RA   Wang S., Chang Y., Guo J., Zeng Q., Ellis B.E., Chen J.G.;
RT   "Arabidopsis ovate family proteins, a novel transcriptional repressor
RT   family, control multiple aspects of plant growth and development.";
RL   PLoS ONE 6:E23896-E23896(2011).
CC   -!- FUNCTION: Transcriptional repressor that regulates multiple
CC       aspects of plant growth and development through the regulation of
CC       BEL1-LIKE (BLH) and KNOX TALE (KNAT) homeodomain transcription
CC       factors. Controls the subcellular localization of the homeodomain
CC       protein BLH1. Plays a role in the regulation of cell elongation by
CC       controlling the expression of GA20OX1, a gene that encodes a key
CC       enzyme in gibberellin biosynthesis. May play a role in double-
CC       stranded DNA repair through the DNA non-homologous end joining
CC       (NHEJ) pathway along with KU70 and KU80 protein complex. Possesses
CC       DNA-binding activity towards double-stranded and single-stranded
CC       DNA in vitro. {ECO:0000269|PubMed:15781858,
CC       ECO:0000269|PubMed:17461792, ECO:0000269|PubMed:20844935,
CC       ECO:0000269|PubMed:21457372, ECO:0000269|PubMed:21886836}.
CC   -!- SUBUNIT: Interacts with ATH1, BLH1, BLH2, BLH3, BLH4, BLH5, BLH10,
CC       KNAT1, KNAT3, KNAT4, KNAT5, KNAT7 and KU70.
CC       {ECO:0000269|PubMed:15781858, ECO:0000269|PubMed:20844935,
CC       ECO:0000269|PubMed:21457372}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15781858,
CC       ECO:0000269|PubMed:17461792, ECO:0000269|PubMed:21457372}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, xylem of stems, flower
CC       buds and siliques. {ECO:0000269|PubMed:17461792,
CC       ECO:0000269|PubMed:21457372, ECO:0000269|PubMed:21886836}.
CC   -!- INDUCTION: By the DNA-damaging agents methyl methanesulfonate
CC       (MMS) and menadione. {ECO:0000269|PubMed:20844935}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC       conditions, but mutant plants are hypersensitive to the DNA-
CC       damaging agents methyl methanesulfonate (MMS) and menadione.
CC       {ECO:0000269|PubMed:15781858, ECO:0000269|PubMed:17461792,
CC       ECO:0000269|PubMed:20844935, ECO:0000269|PubMed:21457372,
CC       ECO:0000269|PubMed:21886836}.
CC   -!- MISCELLANEOUS: Plants over-expressing OFP1 show stunted growth
CC       with general delayed development and shortening and thickening of
CC       all aerial parts. Leaves are irregularly heart-shaped and lobed
CC       and display curved surfaces. Anthers are short with a thick
CC       filament and style and stigma protruded from the flower. Siliques
CC       are short and uneven and produce few seeds (PubMed:15781858 and
CC       PubMed:21886836).
DR   EMBL; AL162351; CAB82754.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED90399.1; -; Genomic_DNA.
DR   PIR; T48205; T48205.
DR   RefSeq; NP_195804.1; NM_120262.3.
DR   UniGene; At.33494; -.
DR   ProteinModelPortal; Q9LZW2; -.
DR   BioGrid; 17089; 16.
DR   IntAct; Q9LZW2; 13.
DR   STRING; 3702.AT5G01840.1; -.
DR   PaxDb; Q9LZW2; -.
DR   EnsemblPlants; AT5G01840.1; AT5G01840.1; AT5G01840.
DR   GeneID; 831813; -.
DR   Gramene; AT5G01840.1; AT5G01840.1; AT5G01840.
DR   KEGG; ath:AT5G01840; -.
DR   Araport; AT5G01840; -.
DR   TAIR; locus:2180977; AT5G01840.
DR   eggNOG; ENOG410IY1K; Eukaryota.
DR   eggNOG; ENOG410YV5M; LUCA.
DR   HOGENOM; HOG000238410; -.
DR   InParanoid; Q9LZW2; -.
DR   OMA; KASDIQF; -.
DR   OrthoDB; EOG09360SL3; -.
DR   PhylomeDB; Q9LZW2; -.
DR   PRO; PR:Q9LZW2; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   Genevisible; Q9LZW2; AT.
DR   GO; GO:0005856; C:cytoskeleton; IDA:TAIR.
DR   GO; GO:0005730; C:nucleolus; IDA:TAIR.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:TAIR.
DR   GO; GO:0051510; P:regulation of unidimensional cell growth; IMP:TAIR.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR   InterPro; IPR025830; DNA_bnd_dom_ovate.
DR   InterPro; IPR006458; Ovate_C.
DR   Pfam; PF13724; DNA_binding_2; 1.
DR   Pfam; PF04844; Ovate; 1.
DR   TIGRFAMs; TIGR01568; A_thal_3678; 1.
DR   PROSITE; PS51754; OVATE; 1.
PE   1: Evidence at protein level;
DR   PRODOM; Q9LZW2.
DR   SWISS-2DPAGE; Q9LZW2.
KW   Complete proteome; DNA damage; DNA repair; DNA-binding; Nucleus;
KW   Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    270       Transcription repressor OFP1.
FT                                /FTId=PRO_0000429670.
FT   DOMAIN      202    261       OVATE. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01090}.
FT   MOTIF        28     45       Nuclear localization signal.
FT                                {ECO:0000255}.
FT   MOTIF       260    264       LxLxL.
SQ   SEQUENCE   270 AA;  30030 MW;  C2A2058809257C39 CRC64;
     MGNNYRFKLS ELIPNAWFYK LRDMSKSKKK NLQSQPNSTT SKKKHHAVPT PTSTTPLSPR
     PPRRPSHSSK APPSHPPRKS SGNRLRHRAT VDSKSSTTSG DSTTTETGSF SPDFRSDQVL
     LPDESLTGSW HSPCSSKLSK TATFTPPPEL ELRPIITKTA ATARKTAVNS PAGVRLRMRS
     PRISVSSSAR RSGSSARRSR AVVKASVDPK RDFKESMEEM IAENKIRATK DLEELLACYL
     CLNSDEYHAI IINVFKQIWL DLNLPPPHSK
//

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