(data stored in ACNUC13479 zone)

SWISSPROT: MAN6_ARATH

ID   MAN6_ARATH              Reviewed;         448 AA.
AC   Q9LZV3;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   07-JUN-2017, entry version 106.
DE   RecName: Full=Mannan endo-1,4-beta-mannosidase 6;
DE            EC=3.2.1.78;
DE   AltName: Full=Beta-mannanase 6;
DE   AltName: Full=Endo-beta-1,4-mannanase 6;
DE            Short=AtMAN6;
DE   Flags: Precursor;
GN   Name=MAN6; OrderedLocusNames=At5g01930; ORFNames=T20L15.200;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Portal (Araport);
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA   Hayashizaki Y., Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY, AND TISSUE SPECIFICITY.
RX   PubMed=16897088; DOI=10.1007/s10142-006-0034-3;
RA   Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.;
RT   "The endo-beta-mannanase gene families in Arabidopsis, rice, and
RT   poplar.";
RL   Funct. Integr. Genomics 7:1-16(2007).
CC   -!- CATALYTIC ACTIVITY: Random hydrolysis of (1->4)-beta-D-mannosidic
CC       linkages in mannans, galactomannans and glucomannans.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16897088}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A)
CC       family. {ECO:0000305}.
DR   EMBL; AL162351; CAB82763.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED90411.1; -; Genomic_DNA.
DR   EMBL; BT005831; AAO64766.1; -; mRNA.
DR   EMBL; AK227325; BAE99339.1; -; mRNA.
DR   PIR; T48214; T48214.
DR   RefSeq; NP_195813.1; NM_120271.4.
DR   UniGene; At.23739; -.
DR   UniGene; At.25601; -.
DR   ProteinModelPortal; Q9LZV3; -.
DR   SMR; Q9LZV3; -.
DR   STRING; 3702.AT5G01930.1; -.
DR   CAZy; GH5; Glycoside Hydrolase Family 5.
DR   PaxDb; Q9LZV3; -.
DR   PRIDE; Q9LZV3; -.
DR   DNASU; 831699; -.
DR   EnsemblPlants; AT5G01930.1; AT5G01930.1; AT5G01930.
DR   GeneID; 831699; -.
DR   Gramene; AT5G01930.1; AT5G01930.1; AT5G01930.
DR   KEGG; ath:AT5G01930; -.
DR   Araport; AT5G01930; -.
DR   TAIR; locus:2180987; AT5G01930.
DR   eggNOG; ENOG410IG6D; Eukaryota.
DR   eggNOG; COG3934; LUCA.
DR   HOGENOM; HOG000169952; -.
DR   InParanoid; Q9LZV3; -.
DR   KO; K19355; -.
DR   OMA; KMTLNST; -.
DR   OrthoDB; EOG09360BHK; -.
DR   PhylomeDB; Q9LZV3; -.
DR   BioCyc; ARA:AT5G01930-MONOMER; -.
DR   BRENDA; 3.2.1.78; 399.
DR   PRO; PR:Q9LZV3; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   Genevisible; Q9LZV3; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR   GO; GO:0046355; P:mannan catabolic process; IBA:GO_Central.
DR   GO; GO:0009845; P:seed germination; IMP:TAIR.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF00150; Cellulase; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00014; ER_TARGET; 1.
PE   2: Evidence at transcript level;
DR   PRODOM; Q9LZV3.
DR   SWISS-2DPAGE; Q9LZV3.
KW   Complete proteome; Glycoprotein; Glycosidase; Hydrolase;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL        1     26       {ECO:0000255}.
FT   CHAIN        27    448       Mannan endo-1,4-beta-mannosidase 6.
FT                                /FTId=PRO_0000277479.
FT   ACT_SITE    219    219       Proton donor. {ECO:0000250}.
FT   ACT_SITE    339    339       Nucleophile. {ECO:0000250}.
FT   CARBOHYD     79     79       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    164    164       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    169    169       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    202    202       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    350    350       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    367    367       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    435    435       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
SQ   SEQUENCE   448 AA;  50622 MW;  977B341312A54D7D CRC64;
     MKDQLGFRIV LCSAVFIILT QNRALADLDS ESHEVNSESV GEEQWEMVQR KGMQFTLNGQ
     PFYVNGFNTY WMMTLAADNS TRGKVTEVFQ QASAVGMTVG RTWAFNDGQW RALQKSPSVY
     DEEVFKALDF VLSEARKYKI RLILSLVNNW DAYGGKAQYV KWGNASGLNL TSDDDFFTNP
     TLRNFYQSHV RTVLNRVNTF TNITYKNDPT IFAWELMNEP RCPSDPSGDK LQSWIQEMAV
     FVKSLDAKHL VEIGLEGFYG PSAPARTRFN PNPYAAQVGT DFIRNNQVLG IDFASVHVYP
     DSWISPAVSN SFLEFTSSWM QAHVEDAEMY LGMPVLFTEF GVSAHDPGFN TSFRDMMLNT
     VYKMTLNSTR KGGAGAGSLV WQVFPQGAEF MDDGYAVYLT RAHTASKIIS LQSKRLAIFN
     SLCSWRCRWG CKKKNQTALD ALLSHDEL
//

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