(data stored in SCRATCH zone)

SWISSPROT: B2JS71_PARP8

ID   B2JS71_PARP8            Unreviewed;       292 AA.
AC   B2JS71;
DT   10-JUN-2008, integrated into UniProtKB/TrEMBL.
DT   10-JUN-2008, sequence version 1.
DT   08-MAY-2019, entry version 56.
DE   RecName: Full=Formyltetrahydrofolate deformylase {ECO:0000256|HAMAP-Rule:MF_01927};
DE            EC=3.5.1.10 {ECO:0000256|HAMAP-Rule:MF_01927};
DE   AltName: Full=Formyl-FH(4) hydrolase {ECO:0000256|HAMAP-Rule:MF_01927};
GN   Name=purU {ECO:0000256|HAMAP-Rule:MF_01927};
GN   OrderedLocusNames=Bphy_3292 {ECO:0000313|EMBL:ACC72448.1};
OS   Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 /
OS   STM815) (Burkholderia phymatum).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=391038 {ECO:0000313|EMBL:ACC72448.1, ECO:0000313|Proteomes:UP000001192};
RN   [1] {ECO:0000313|Proteomes:UP000001192}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17167 / CIP 108236 / LMG 21445 / STM815
RC   {ECO:0000313|Proteomes:UP000001192};
RX   PubMed=25197461; DOI=10.4056/sigs.4861021;
RA   Moulin L., Klonowska A., Caroline B., Booth K., Vriezen J.A.,
RA   Melkonian R., James E.K., Young J.P., Bena G., Hauser L., Land M.,
RA   Kyrpides N., Bruce D., Chain P., Copeland A., Pitluck S., Woyke T.,
RA   Lizotte-Waniewski M., Bristow J., Riley M.;
RT   "Complete genome sequence of Burkholderia phymatum STM815(T), a broad
RT   host range and efficient nitrogen-fixing symbiont of Mimosa species.";
RL   Stand. Genomic Sci. 9:763-774(2014).
CC   -!- FUNCTION: Catalyzes the hydrolysis of 10-formyltetrahydrofolate
CC       (formyl-FH4) to formate and tetrahydrofolate (FH4).
CC       {ECO:0000256|HAMAP-Rule:MF_01927}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-10-formyltetrahydrofolate + H2O = (6S)-5,6,7,8-
CC         tetrahydrofolate + formate + H(+); Xref=Rhea:RHEA:19833,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454; EC=3.5.1.10;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01927};
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       formate from 10-formyl-5,6,7,8-tetrahydrofolate: step 1/1.
CC       {ECO:0000256|HAMAP-Rule:MF_01927}.
CC   -!- SIMILARITY: Belongs to the PurU family. {ECO:0000256|HAMAP-
CC       Rule:MF_01927}.
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DR   EMBL; CP001044; ACC72448.1; -; Genomic_DNA.
DR   RefSeq; WP_012402621.1; NC_010623.1.
DR   STRING; 391038.Bphy_3292; -.
DR   EnsemblBacteria; ACC72448; ACC72448; Bphy_3292.
DR   GeneID; 27742978; -.
DR   KEGG; bph:Bphy_3292; -.
DR   eggNOG; ENOG4105CIY; Bacteria.
DR   eggNOG; COG0788; LUCA.
DR   HOGENOM; HOG000033576; -.
DR   KO; K01433; -.
DR   OMA; FLERHRC; -.
DR   OrthoDB; 979667at2; -.
DR   BioCyc; BPHY391038:G1GBS-3388-MONOMER; -.
DR   UniPathway; UPA00074; UER00170.
DR   Proteomes; UP000001192; Chromosome 2.
DR   GO; GO:0008864; F:formyltetrahydrofolate deformylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016742; F:hydroxymethyl-, formyl- and related transferase activity; IEA:InterPro.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd08648; FMT_core_Formyl-FH4-Hydrolase_; 1.
DR   HAMAP; MF_01927; PurU; 1.
DR   InterPro; IPR041729; Formyl-FH4-Hydrolase_C.
DR   InterPro; IPR002376; Formyl_transf_N.
DR   InterPro; IPR036477; Formyl_transf_N_sf.
DR   InterPro; IPR004810; PurU.
DR   PANTHER; PTHR42706; PTHR42706; 1.
DR   Pfam; PF00551; Formyl_trans_N; 1.
DR   PRINTS; PR01575; FFH4HYDRLASE.
DR   SUPFAM; SSF53328; SSF53328; 1.
DR   TIGRFAMs; TIGR00655; PurU; 1.
PE   3: Inferred from homology;
DR   PRODOM; B2JS71.
DR   SWISS-2DPAGE; B2JS71.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001192};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_01927};
KW   One-carbon metabolism {ECO:0000256|HAMAP-Rule:MF_01927};
KW   Purine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01927};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001192}.
FT   DOMAIN       98    274       Formyl_trans_N. {ECO:0000259|Pfam:
FT                                PF00551}.
FT   ACT_SITE    237    237       {ECO:0000256|HAMAP-Rule:MF_01927}.
SQ   SEQUENCE   292 AA;  32850 MW;  0D1BD5822DB64EDC CRC64;
     MPATDRHPQF ALTLSCPSAA GQVAAIVGFL DRHHCYIDEL TVFDDDISER FFVRCVFHGV
     GSAPDAALHI DTLRHEFRPI AADFDMTWAM HDLEMRPKVL IMVSKLEHCL ADLLFRWRMG
     ELKMDIAGIA SNHPDFQPLA AQHGLPFHHF PLTPDTKAQQ EAQILDLFDK SGAELMILAR
     YMQILSDETS RKLSGRAINI HHSFLPGFKG ARPYHQAHAR GVKLIGATAH FVTDDLDEGP
     IIEQEVQRVD HSYGPERMLA VGRDVECITL ARAVKAFVER RVFINGDRTV VL
//

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