(data stored in SCRATCH zone)

SWISSPROT: B2UI78_RALPJ

ID   B2UI78_RALPJ            Unreviewed;       187 AA.
AC   B2UI78;
DT   01-JUL-2008, integrated into UniProtKB/TrEMBL.
DT   01-JUL-2008, sequence version 1.
DT   08-MAY-2019, entry version 62.
DE   RecName: Full=Lipid A deacylase {ECO:0000256|PIRNR:PIRNR029681};
DE            EC=3.1.1.77 {ECO:0000256|PIRNR:PIRNR029681};
DE   AltName: Full=LPS 3-O-deacylase {ECO:0000256|PIRNR:PIRNR029681};
DE   AltName: Full=Outer membrane enzyme {ECO:0000256|PIRNR:PIRNR029681};
GN   OrderedLocusNames=Rpic_4180 {ECO:0000313|EMBL:ACD29279.1};
OS   Ralstonia pickettii (strain 12J).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=402626 {ECO:0000313|EMBL:ACD29279.1, ECO:0000313|Proteomes:UP000002566};
RN   [1] {ECO:0000313|Proteomes:UP000002566}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=12J {ECO:0000313|Proteomes:UP000002566};
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T.,
RA   Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Marsh T., Richardson P.;
RT   "Complete sequence of chromosome 2 of Ralstonia pickettii 12J.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Has lipid A 3-O-deacylase activity. Hydrolyzes the ester
CC       bond at the 3 position of lipid A, a bioactive component of
CC       lipopolysaccharide (LPS), thereby releasing the primary fatty acyl
CC       moiety. {ECO:0000256|PIRNR:PIRNR029681}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 3-(acyloxy)acyl derivative of bacterial toxin + H2O =
CC         3-hydroxyacyl derivative of bacterial toxin + a fatty acid +
CC         H(+); Xref=Rhea:RHEA:12032, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:136853,
CC         ChEBI:CHEBI:140675; EC=3.1.1.77;
CC         Evidence={ECO:0000256|PIRNR:PIRNR029681};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|PIRNR:PIRNR029681}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane
CC       {ECO:0000256|PIRNR:PIRNR029681}; Multi-pass membrane protein
CC       {ECO:0000256|PIRNR:PIRNR029681}.
CC   -!- SIMILARITY: Belongs to the PagL family.
CC       {ECO:0000256|PIRNR:PIRNR029681}.
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DR   EMBL; CP001069; ACD29279.1; -; Genomic_DNA.
DR   RefSeq; WP_004627504.1; NC_010678.1.
DR   STRING; 402626.Rpic_4180; -.
DR   EnsemblBacteria; ACD29279; ACD29279; Rpic_4180.
DR   GeneID; 6285606; -.
DR   KEGG; rpi:Rpic_4180; -.
DR   PATRIC; fig|402626.5.peg.428; -.
DR   eggNOG; ENOG41061N2; Bacteria.
DR   eggNOG; ENOG4112AQW; LUCA.
DR   HOGENOM; HOG000264842; -.
DR   OMA; AYTYWDG; -.
DR   OrthoDB; 1863236at2; -.
DR   BioCyc; RPIC402626:GH94-4175-MONOMER; -.
DR   Proteomes; UP000002566; Chromosome 2.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050528; F:acyloxyacyl hydrolase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR018550; Lipid-A_deacylase-rel.
DR   InterPro; IPR011250; OMP/PagP_b-brl.
DR   Pfam; PF09411; PagL; 1.
DR   PIRSF; PIRSF029681; PagL; 1.
DR   SUPFAM; SSF56925; SSF56925; 1.
PE   3: Inferred from homology;
DR   PRODOM; B2UI78.
DR   SWISS-2DPAGE; B2UI78.
KW   Cell outer membrane {ECO:0000256|PIRNR:PIRNR029681};
KW   Complete proteome {ECO:0000313|Proteomes:UP000002566};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR029681};
KW   Membrane {ECO:0000256|PIRNR:PIRNR029681};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002566};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL        1     30       {ECO:0000256|SAM:SignalP}.
FT   CHAIN        31    187       Lipid A deacylase. {ECO:0000256|SAM:
FT                                SignalP}.
FT                                /FTId=PRO_5002783822.
FT   ACT_SITE    163    163       Charge relay system. {ECO:0000256|PIRSR:
FT                                PIRSR029681-1}.
FT   ACT_SITE    165    165       Charge relay system. {ECO:0000256|PIRSR:
FT                                PIRSR029681-1}.
FT   ACT_SITE    177    177       Charge relay system. {ECO:0000256|PIRSR:
FT                                PIRSR029681-1}.
FT   SITE        166    166       Critical for activity.
FT                                {ECO:0000256|PIRSR:PIRSR029681-2}.
SQ   SEQUENCE   187 AA;  20821 MW;  2B765E74FF27D7DB CRC64;
     MIRSALPRSA KLLAAALGAA TLFAALPAQA DPSVRAIYGK DNKHGIEKYG VDLDFDSGFH
     WGNPEGWFVN LDWEVALGQW RSTKGTNRQN ITEFGVTPLF RLEKRGGSWV PFIEAGVGPR
     LLSHVSTSNE HYFSTAFQFS DMIGVGVAFG SKQQFQMGYR FEHLSNASIK RPNPGTDLNE
     LYLRYTF
//

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