(data stored in SCRATCH zone)

SWISSPROT: B7UKP0_ECO27

ID   B7UKP0_ECO27            Unreviewed;       137 AA.
AC   B7UKP0;
DT   10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT   10-FEB-2009, sequence version 1.
DT   08-MAY-2019, entry version 53.
DE   RecName: Full=Proofreading thioesterase EntH {ECO:0000256|HAMAP-Rule:MF_00907};
DE            EC=3.1.2.- {ECO:0000256|HAMAP-Rule:MF_00907};
DE   AltName: Full=Enterobactin synthase component H {ECO:0000256|HAMAP-Rule:MF_00907};
GN   Name=ybdB {ECO:0000313|EMBL:CAS08047.1};
GN   Synonyms=entH {ECO:0000256|HAMAP-Rule:MF_00907};
GN   OrderedLocusNames=E2348C_0499 {ECO:0000313|EMBL:CAS08047.1};
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521 {ECO:0000313|EMBL:CAS08047.1, ECO:0000313|Proteomes:UP000008205};
RN   [1] {ECO:0000313|EMBL:CAS08047.1, ECO:0000313|Proteomes:UP000008205}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC {ECO:0000313|Proteomes:UP000008205};
RX   PubMed=18952797; DOI=10.1128/JB.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T.,
RA   Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P.,
RA   Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J.,
RA   Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- FUNCTION: Required for optimal enterobactin synthesis. Acts as a
CC       proofreading enzyme that prevents EntB misacylation by hydrolyzing
CC       the thioester bound existing between EntB and wrongly charged
CC       molecules. {ECO:0000256|HAMAP-Rule:MF_00907}.
CC   -!- PATHWAY: Siderophore biosynthesis; enterobactin biosynthesis.
CC       {ECO:0000256|HAMAP-Rule:MF_00907}.
CC   -!- SUBUNIT: Homotetramer. Dimer of dimers. Interacts specifically
CC       with the aryl carrier protein (ArCP) domain of EntB.
CC       {ECO:0000256|HAMAP-Rule:MF_00907}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00907}.
CC   -!- SIMILARITY: Belongs to the thioesterase PaaI family.
CC       {ECO:0000256|HAMAP-Rule:MF_00907}.
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DR   EMBL; FM180568; CAS08047.1; -; Genomic_DNA.
DR   RefSeq; WP_000637954.1; NC_011601.1.
DR   EnsemblBacteria; CAS08047; CAS08047; E2348C_0499.
DR   KEGG; ecg:E2348C_0499; -.
DR   HOGENOM; HOG000066991; -.
DR   OMA; HGGVYCS; -.
DR   BioCyc; ECOL574521:E2348C_RS02625-MONOMER; -.
DR   UniPathway; UPA00017; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016790; F:thiolester hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009239; P:enterobactin biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00907; Thioesterase_EntH; 1.
DR   InterPro; IPR029069; HotDog_dom_sf.
DR   InterPro; IPR003736; PAAI_dom.
DR   InterPro; IPR026576; Thioesterase_EntH.
DR   InterPro; IPR006683; Thioestr_dom.
DR   Pfam; PF03061; 4HBT; 1.
DR   SUPFAM; SSF54637; SSF54637; 1.
DR   TIGRFAMs; TIGR00369; unchar_dom_1; 1.
PE   3: Inferred from homology;
DR   PRODOM; B7UKP0.
DR   SWISS-2DPAGE; B7UKP0.
KW   Complete proteome {ECO:0000313|Proteomes:UP000008205};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00907};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00907}.
FT   DOMAIN       50    127       4HBT. {ECO:0000259|Pfam:PF03061}.
FT   ACT_SITE     63     63       Nucleophile or proton acceptor.
FT                                {ECO:0000256|HAMAP-Rule:MF_00907}.
SQ   SEQUENCE   137 AA;  14998 MW;  754F8DE63815F205 CRC64;
     MIWKRHLTLD ELNATSDNTM VAHLGIVYTR LGDDVLEAEM PVDTRTHQPF GLLHGGASAA
     LAETLGSMAG FMMTRDGQCV VGTELNATHH RPVSEGKVRG VCQPLHLGRQ NQSWEIVVFD
     ERGRRCCTCR LGTAVLG
//

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