(data stored in SCRATCH zone)

SWISSPROT: B7UKW9_ECO27

ID   B7UKW9_ECO27            Unreviewed;       181 AA.
AC   B7UKW9;
DT   10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT   10-FEB-2009, sequence version 1.
DT   08-MAY-2019, entry version 60.
DE   RecName: Full=Negative modulator of initiation of replication {ECO:0000256|HAMAP-Rule:MF_00908, ECO:0000256|PIRNR:PIRNR019401};
GN   Name=seqA {ECO:0000256|HAMAP-Rule:MF_00908,
GN   ECO:0000313|EMBL:CAS08127.1};
GN   OrderedLocusNames=E2348C_0579 {ECO:0000313|EMBL:CAS08127.1};
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521 {ECO:0000313|EMBL:CAS08127.1, ECO:0000313|Proteomes:UP000008205};
RN   [1] {ECO:0000313|EMBL:CAS08127.1, ECO:0000313|Proteomes:UP000008205}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC {ECO:0000313|Proteomes:UP000008205};
RX   PubMed=18952797; DOI=10.1128/JB.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T.,
RA   Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P.,
RA   Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J.,
RA   Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- FUNCTION: Negative regulator of replication initiation, which
CC       contributes to regulation of DNA replication and ensures that
CC       replication initiation occurs exactly once per chromosome per cell
CC       cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC       region, thus preventing assembly of replication proteins and re-
CC       initiation at newly replicated origins. Repression is relieved
CC       when the region becomes fully methylated. {ECO:0000256|HAMAP-
CC       Rule:MF_00908, ECO:0000256|PIRNR:PIRNR019401}.
CC   -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC       {ECO:0000256|HAMAP-Rule:MF_00908}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00908,
CC       ECO:0000256|PIRNR:PIRNR019401}.
CC   -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000256|HAMAP-
CC       Rule:MF_00908, ECO:0000256|PIRNR:PIRNR019401}.
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DR   EMBL; FM180568; CAS08127.1; -; Genomic_DNA.
DR   RefSeq; WP_000848387.1; NC_011601.1.
DR   SMR; B7UKW9; -.
DR   EnsemblBacteria; CAS08127; CAS08127; E2348C_0579.
DR   KEGG; ecg:E2348C_0579; -.
DR   HOGENOM; HOG000275226; -.
DR   KO; K03645; -.
DR   OMA; RTRTYFA; -.
DR   BioCyc; ECOL574521:E2348C_RS03070-MONOMER; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0032297; P:negative regulation of DNA-dependent DNA replication initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   Gene3D; 1.20.1380.10; -; 1.
DR   HAMAP; MF_00908; SeqA; 1.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   InterPro; IPR005621; SeqA.
DR   InterPro; IPR026577; SeqA_DNA-bd_C.
DR   InterPro; IPR036835; SeqA_DNA-bd_C_sf.
DR   InterPro; IPR033761; SeqA_N.
DR   Pfam; PF03925; SeqA; 1.
DR   Pfam; PF17206; SeqA_N; 1.
DR   PIRSF; PIRSF019401; SeqA; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
DR   SUPFAM; SSF82808; SSF82808; 1.
PE   3: Inferred from homology;
DR   PRODOM; B7UKW9.
DR   SWISS-2DPAGE; B7UKW9.
KW   Complete proteome {ECO:0000313|Proteomes:UP000008205};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00908,
KW   ECO:0000256|PIRNR:PIRNR019401};
KW   DNA replication inhibitor {ECO:0000256|HAMAP-Rule:MF_00908,
KW   ECO:0000256|PIRNR:PIRNR019401};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00908,
KW   ECO:0000256|PIRNR:PIRNR019401, ECO:0000256|SAAS:SAAS00993492}.
FT   DOMAIN        1     35       SeqA_N. {ECO:0000259|Pfam:PF17206}.
FT   DOMAIN       71    179       SeqA. {ECO:0000259|Pfam:PF03925}.
FT   REGION       87     88       Interaction with DNA. {ECO:0000256|HAMAP-
FT                                Rule:MF_00908}.
FT   REGION      116    120       Interaction with DNA. {ECO:0000256|HAMAP-
FT                                Rule:MF_00908}.
FT   REGION      150    156       Interaction with DNA. {ECO:0000256|HAMAP-
FT                                Rule:MF_00908}.
SQ   SEQUENCE   181 AA;  20315 MW;  37D9078CA3D7229B CRC64;
     MKTIEVDDEL YSYIASHTKH IGESASDILR RMLKFSAASQ PAAPVTKEVR VASPAIVEAK
     PVKTIKDKVR AMRELLLSDE YAEQKRAVNR FMLLLSTLYS LDAQAFAEAT ESLHGRTRVY
     FAADEQTLLK NGNQTKPKHV PGTPYWVITN TNTGRKCSMI EHIMQSMQFP AELIEKVCGT
     I
//

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