(data stored in SCRATCH zone)

SWISSPROT: B7UKX1_ECO27

ID   B7UKX1_ECO27            Unreviewed;       439 AA.
AC   B7UKX1;
DT   10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT   10-FEB-2009, sequence version 1.
DT   08-MAY-2019, entry version 60.
DE   RecName: Full=Putrescine transporter PotE {ECO:0000256|HAMAP-Rule:MF_02073};
DE   AltName: Full=Putrescine-proton symporter / putrescine-ornithine antiporter {ECO:0000256|HAMAP-Rule:MF_02073};
GN   Name=potE {ECO:0000256|HAMAP-Rule:MF_02073,
GN   ECO:0000313|EMBL:CAS08129.1};
GN   OrderedLocusNames=E2348C_0581 {ECO:0000313|EMBL:CAS08129.1};
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521 {ECO:0000313|EMBL:CAS08129.1, ECO:0000313|Proteomes:UP000008205};
RN   [1] {ECO:0000313|EMBL:CAS08129.1, ECO:0000313|Proteomes:UP000008205}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC {ECO:0000313|Proteomes:UP000008205};
RX   PubMed=18952797; DOI=10.1128/JB.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T.,
RA   Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P.,
RA   Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J.,
RA   Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- FUNCTION: Catalyzes both the uptake and excretion of putrescine.
CC       The uptake of putrescine is dependent on the membrane potential
CC       and the excretion involves putrescine-ornithine antiporter
CC       activity. {ECO:0000256|HAMAP-Rule:MF_02073}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC       Rule:MF_02073}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC       Rule:MF_02073}.
CC   -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC       superfamily. Basic amino acid/polyamine antiporter (APA)
CC       (TC 2.A.3.2) family. {ECO:0000256|HAMAP-Rule:MF_02073}.
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DR   EMBL; FM180568; CAS08129.1; -; Genomic_DNA.
DR   RefSeq; WP_000075845.1; NC_011601.1.
DR   EnsemblBacteria; CAS08129; CAS08129; E2348C_0581.
DR   KEGG; ecg:E2348C_0581; -.
DR   HOGENOM; HOG000164507; -.
DR   KO; K03756; -.
DR   OMA; AEKVMSV; -.
DR   BioCyc; ECOL574521:E2348C_RS03080-MONOMER; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0015496; F:putrescine:ornithine antiporter activity; IEA:InterPro.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR   HAMAP; MF_02073; Putrescine_transp; 1.
DR   InterPro; IPR002293; AA/rel_permease1.
DR   InterPro; IPR004754; Amino_acid_antiprt.
DR   InterPro; IPR027566; Symport/antiport_PotE.
DR   Pfam; PF13520; AA_permease_2; 1.
DR   PIRSF; PIRSF006060; AA_transporter; 1.
DR   TIGRFAMs; TIGR00905; 2A0302; 1.
DR   TIGRFAMs; TIGR04299; antiport_PotE; 1.
PE   3: Inferred from homology;
DR   PRODOM; B7UKX1.
DR   SWISS-2DPAGE; B7UKX1.
KW   Amino-acid transport {ECO:0000256|HAMAP-Rule:MF_02073};
KW   Antiport {ECO:0000256|HAMAP-Rule:MF_02073};
KW   Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_02073};
KW   Cell membrane {ECO:0000256|HAMAP-Rule:MF_02073};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008205};
KW   Membrane {ECO:0000256|HAMAP-Rule:MF_02073};
KW   Symport {ECO:0000256|HAMAP-Rule:MF_02073};
KW   Transmembrane {ECO:0000256|HAMAP-Rule:MF_02073};
KW   Transmembrane helix {ECO:0000256|HAMAP-Rule:MF_02073};
KW   Transport {ECO:0000256|HAMAP-Rule:MF_02073}.
FT   TRANSMEM     12     30       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
FT   TRANSMEM     42     60       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
FT   TRANSMEM     90    110       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
FT   TRANSMEM    122    140       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
FT   TRANSMEM    152    172       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
FT   TRANSMEM    184    204       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
FT   TRANSMEM    225    249       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
FT   TRANSMEM    276    300       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
FT   TRANSMEM    321    345       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
FT   TRANSMEM    351    374       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
FT   TRANSMEM    386    404       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
FT   TRANSMEM    410    428       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_02073}.
SQ   SEQUENCE   439 AA;  46495 MW;  C6F800284DA8C5C8 CRC64;
     MSQAKSNKMG VVQLTILTMV NMMGSGIIML PTKLAEVGTI SIISWLVTAV GSMALAWAFA
     KCGMFSRKSG GMGGYAEYAF GKSGNFMANY TYGVSLLIAN VAIAISAVGY GTELLGASLS
     PVQIGLATIG VLWICTVANF GGARITGQIS SITVWGVIIP VVGLCIIGWF WFSPTLYVDS
     WNPHHAPFFS AVGSSIAMTL WAFLGLESAC ANTDVVENPE RNVPIAVLGG TLGAAVIYIV
     STNVIAGIVP NMELANSTAP FGLAFAQMFT PEVGKVIMAL MVMSCCGSLL GWQFTIAQVF
     KSSSDEGYFP KIFSRVTKVD APVQGMLTIV IIQSGLALMT ISPSLNSQFN VLVNLAVVTN
     IIPYILSMAA LVIIQKVANV PPSKAKVANF VAFVGAMYSF YALYSSGEEA MLYGSIVTFL
     GWTLYGLVSP RFELKNKHG
//

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