(data stored in SCRATCH zone)

SWISSPROT: B7ULP5_ECO27

ID   B7ULP5_ECO27            Unreviewed;       427 AA.
AC   B7ULP5;
DT   10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT   10-FEB-2009, sequence version 1.
DT   08-MAY-2019, entry version 54.
DE   RecName: Full=Pectinesterase {ECO:0000256|RuleBase:RU000589};
DE            EC=3.1.1.11 {ECO:0000256|RuleBase:RU000589};
GN   Name=ybhC {ECO:0000313|EMBL:CAS08197.1};
GN   OrderedLocusNames=E2348C_0649 {ECO:0000313|EMBL:CAS08197.1};
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521 {ECO:0000313|EMBL:CAS08197.1, ECO:0000313|Proteomes:UP000008205};
RN   [1] {ECO:0000313|EMBL:CAS08197.1, ECO:0000313|Proteomes:UP000008205}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC {ECO:0000313|Proteomes:UP000008205};
RX   PubMed=18952797; DOI=10.1128/JB.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T.,
RA   Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P.,
RA   Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J.,
RA   Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O =
CC         [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol;
CC         Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-
CC         COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523;
CC         EC=3.1.1.11; Evidence={ECO:0000256|RuleBase:RU000589};
CC   -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-
CC       D-gluconate from pectin: step 1/5.
CC       {ECO:0000256|RuleBase:RU000589}.
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DR   EMBL; FM180568; CAS08197.1; -; Genomic_DNA.
DR   RefSeq; WP_001091562.1; NC_011601.1.
DR   EnsemblBacteria; CAS08197; CAS08197; E2348C_0649.
DR   KEGG; ecg:E2348C_0649; -.
DR   HOGENOM; HOG000118057; -.
DR   KO; K01051; -.
DR   OMA; FNRMWEY; -.
DR   BioCyc; ECOL574521:E2348C_RS03485-MONOMER; -.
DR   UniPathway; UPA00545; UER00823.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0045330; F:aspartyl esterase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030599; F:pectinesterase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042545; P:cell wall modification; IEA:UniProtKB-UniRule.
DR   GO; GO:0045490; P:pectin catabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   InterPro; IPR033131; Pectinesterase_Asp_AS.
DR   InterPro; IPR000070; Pectinesterase_cat.
DR   Pfam; PF01095; Pectinesterase; 1.
DR   SUPFAM; SSF51126; SSF51126; 1.
DR   PROSITE; PS00503; PECTINESTERASE_2; 1.
PE   4: Predicted;
DR   PRODOM; B7ULP5.
DR   SWISS-2DPAGE; B7ULP5.
KW   Aspartyl esterase {ECO:0000256|RuleBase:RU000589};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008205};
KW   Hydrolase {ECO:0000256|RuleBase:RU000589};
KW   Signal {ECO:0000256|RuleBase:RU000589}.
FT   SIGNAL        1     23       {ECO:0000256|RuleBase:RU000589}.
FT   CHAIN        24    427       Pectinesterase. {ECO:0000256|RuleBase:
FT                                RU000589}.
FT                                /FTId=PRO_5005123890.
FT   DOMAIN      194    339       Pectinesterase. {ECO:0000259|Pfam:
FT                                PF01095}.
FT   ACT_SITE    285    285       {ECO:0000256|PROSITE-ProRule:PRU10040}.
SQ   SEQUENCE   427 AA;  46008 MW;  3DB4A1BFA4358B1D CRC64;
     MNTFSVSRLA LALAFGVTLT ACSSTPPDQR PSDQTAPGTS SRPILSAKEA QNFDAQHYFA
     SLTPGAAAWN PSPITLPAQP DFVVGPAGTQ GVTHTTIQAA VDAAIIKRTN KRQYIAVMPG
     EYQGTVYVPA APGGITLYGT GEKPIDVKIG LSLDGGMSPA DWRHDVNPRG KYMPGKPAWY
     MYDSCQSKRS DSIGVLCSAV FWSQNNGLQL QNLTIENTLG DSVDAGNHPA VALRTDGDKV
     QINNVNILGR QNTFFVTNSG VQNRLETNRQ PRTLVTNSYI EGDVDIVSGR GAVVFDNTEF
     RVVNSRTQQE AYVFAPATLS NIYYGFLAVN SRFNASGDGV AQLGRSLDVD ANTNGQVVIR
     DSAINEGFNT AKPWADAVIS NRPFAGNTGS VDDNDEVQRN LNDTNYNRMW EYNNRGVGSK
     VVAEAKK
//

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