(data stored in SCRATCH zone)

SWISSPROT: B8IB16_METNO

ID   B8IB16_METNO            Unreviewed;       377 AA.
AC   B8IB16;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   07-JUN-2017, entry version 59.
DE   RecName: Full=Putative glutamate--cysteine ligase 2 {ECO:0000256|HAMAP-Rule:MF_01609};
DE            EC=6.3.2.2 {ECO:0000256|HAMAP-Rule:MF_01609};
DE   AltName: Full=Gamma-glutamylcysteine synthetase 2 {ECO:0000256|HAMAP-Rule:MF_01609};
DE            Short=GCS 2 {ECO:0000256|HAMAP-Rule:MF_01609};
DE            Short=Gamma-GCS 2 {ECO:0000256|HAMAP-Rule:MF_01609};
GN   OrderedLocusNames=Mnod_0366 {ECO:0000313|EMBL:ACL55409.1};
OS   Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=460265 {ECO:0000313|EMBL:ACL55409.1, ECO:0000313|Proteomes:UP000008207};
RN   [1] {ECO:0000313|EMBL:ACL55409.1, ECO:0000313|Proteomes:UP000008207}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 21967 / CNCM I-2342 / ORS 2060
RC   {ECO:0000313|Proteomes:UP000008207};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T.,
RA   Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Ivanova N., Marx C.J., Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium nodulans ORS
RT   2060.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: ATP-dependent carboxylate-amine ligase which exhibits
CC       weak glutamate--cysteine ligase activity. {ECO:0000256|HAMAP-
CC       Rule:MF_01609}.
CC   -!- CATALYTIC ACTIVITY: ATP + L-glutamate + L-cysteine = ADP +
CC       phosphate + gamma-L-glutamyl-L-cysteine. {ECO:0000256|HAMAP-
CC       Rule:MF_01609, ECO:0000256|SAAS:SAAS00378060}.
CC   -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 2
CC       family. YbdK subfamily. {ECO:0000256|HAMAP-Rule:MF_01609,
CC       ECO:0000256|SAAS:SAAS00809443}.
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DR   EMBL; CP001349; ACL55409.1; -; Genomic_DNA.
DR   RefSeq; WP_015927120.1; NC_011894.1.
DR   ProteinModelPortal; B8IB16; -.
DR   STRING; 460265.Mnod_0366; -.
DR   EnsemblBacteria; ACL55409; ACL55409; Mnod_0366.
DR   KEGG; mno:Mnod_0366; -.
DR   eggNOG; COG2170; LUCA.
DR   HOGENOM; HOG000220943; -.
DR   KO; K06048; -.
DR   OMA; LIFGLHV; -.
DR   OrthoDB; POG091H09O7; -.
DR   Proteomes; UP000008207; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042398; P:cellular modified amino acid biosynthetic process; IEA:InterPro.
DR   HAMAP; MF_01609; Glu_cys_ligase_2; 1.
DR   InterPro; IPR006336; GCS2.
DR   InterPro; IPR011793; YbdK.
DR   Pfam; PF04107; GCS2; 1.
DR   TIGRFAMs; TIGR02050; gshA_cyan_rel; 1.
PE   3: Inferred from homology;
DR   PRODOM; B8IB16.
DR   SWISS-2DPAGE; B8IB16.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_01609,
KW   ECO:0000256|SAAS:SAAS00078237};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008207};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_01609,
KW   ECO:0000256|SAAS:SAAS00078241, ECO:0000313|EMBL:ACL55409.1};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01609,
KW   ECO:0000256|SAAS:SAAS00078244};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008207}.
SQ   SEQUENCE   377 AA;  41316 MW;  A2C5DE3385029B48 CRC64;
     MHADFAFCIE EDVFLNDAQK RDAARARVRD FLAVCRRDFP AARHDLIEPR LIWTTPPLAD
     LAQARRVLAE LRGGIGALAA TQGLAVMAAG THPIALWSRV RPRDTGARDR VQRDLQMVGS
     RTVVCGLSIR VGLPDGVSRI DVMNRIQPFL PLLLALSTSS PFWQAQRTGL LGYRLAASRE
     LPRSGLPPYF ADEADHARYL DALMRAGAID DLGHVWWVIR PAPQGNALDL RIADSCTRLD
     DTLAIAALTR CLVRRLARDP ALNRDLTGAT HAITAENCWR AQRYGIHGSF MAEEEAAMPV
     KEVLARTLAL LAEDAAALGC TAELDLARWI VARGTSADRQ LALFTEAQGR GLTQREALCT
     VVDWLSAETV GAAPTRH
//

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