(data stored in SCRATCH zone)

SWISSPROT: B8IB31_METNO

ID   B8IB31_METNO            Unreviewed;       183 AA.
AC   B8IB31;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   07-JUN-2017, entry version 66.
DE   RecName: Full=3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase {ECO:0000256|HAMAP-Rule:MF_00405};
DE            EC=4.2.1.59 {ECO:0000256|HAMAP-Rule:MF_00405};
DE   AltName: Full=3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA {ECO:0000256|HAMAP-Rule:MF_00405};
DE   AltName: Full=Beta-hydroxydecanoyl thioester dehydrase {ECO:0000256|HAMAP-Rule:MF_00405};
DE   AltName: Full=Trans-2-decenoyl-[acyl-carrier-protein] isomerase {ECO:0000256|HAMAP-Rule:MF_00405};
DE            EC=5.3.3.14 {ECO:0000256|HAMAP-Rule:MF_00405};
GN   Name=fabA {ECO:0000256|HAMAP-Rule:MF_00405};
GN   OrderedLocusNames=Mnod_0382 {ECO:0000313|EMBL:ACL55424.1};
OS   Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=460265 {ECO:0000313|EMBL:ACL55424.1, ECO:0000313|Proteomes:UP000008207};
RN   [1] {ECO:0000313|EMBL:ACL55424.1, ECO:0000313|Proteomes:UP000008207}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 21967 / CNCM I-2342 / ORS 2060
RC   {ECO:0000313|Proteomes:UP000008207};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T.,
RA   Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Ivanova N., Marx C.J., Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium nodulans ORS
RT   2060.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Necessary for the introduction of cis unsaturation into
CC       fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-
CC       ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-
CC       decenoyl-ACP. Can catalyze the dehydratase reaction for beta-
CC       hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being
CC       most active on intermediate chain length. {ECO:0000256|HAMAP-
CC       Rule:MF_00405, ECO:0000256|SAAS:SAAS00699089}.
CC   -!- CATALYTIC ACTIVITY: (3R)-3-hydroxydecanoyl-[acyl-carrier-protein]
CC       = trans-dec-2-enoyl-[acyl-carrier-protein] + H(2)O.
CC       {ECO:0000256|HAMAP-Rule:MF_00405, ECO:0000256|SAAS:SAAS00699057}.
CC   -!- CATALYTIC ACTIVITY: A (3R)-3-hydroxyacyl-[acyl-carrier protein] =
CC       a trans-2-enoyl-[acyl-carrier protein] + H(2)O.
CC       {ECO:0000256|HAMAP-Rule:MF_00405, ECO:0000256|SAAS:SAAS00699093}.
CC   -!- CATALYTIC ACTIVITY: Trans-dec-2-enoyl-[acyl-carrier-protein] =
CC       cis-dec-3-enoyl-[acyl-carrier-protein]. {ECO:0000256|HAMAP-
CC       Rule:MF_00405, ECO:0000256|SAAS:SAAS00699076}.
CC   -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC       {ECO:0000256|HAMAP-Rule:MF_00405, ECO:0000256|SAAS:SAAS00699065}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00405,
CC       ECO:0000256|SAAS:SAAS00699100}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00405,
CC       ECO:0000256|SAAS:SAAS00699074}.
CC   -!- SIMILARITY: Belongs to the thioester dehydratase family. FabA
CC       subfamily. {ECO:0000256|HAMAP-Rule:MF_00405,
CC       ECO:0000256|SAAS:SAAS00699085}.
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DR   EMBL; CP001349; ACL55424.1; -; Genomic_DNA.
DR   RefSeq; WP_015927135.1; NC_011894.1.
DR   ProteinModelPortal; B8IB31; -.
DR   STRING; 460265.Mnod_0382; -.
DR   EnsemblBacteria; ACL55424; ACL55424; Mnod_0382.
DR   KEGG; mno:Mnod_0382; -.
DR   eggNOG; ENOG4108RKI; Bacteria.
DR   eggNOG; COG0764; LUCA.
DR   HOGENOM; HOG000277828; -.
DR   KO; K01716; -.
DR   OMA; FFDCHFK; -.
DR   OrthoDB; POG091H0666; -.
DR   UniPathway; UPA00094; -.
DR   Proteomes; UP000008207; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008693; F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0047451; F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0034017; F:trans-2-decenoyl-acyl-carrier-protein isomerase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-HAMAP.
DR   CDD; cd01287; FabA; 1.
DR   HAMAP; MF_00405; FabA; 1.
DR   InterPro; IPR010083; FabA.
DR   InterPro; IPR013114; FabA_FabZ.
DR   InterPro; IPR029069; HotDog_dom.
DR   PANTHER; PTHR30272:SF10; PTHR30272:SF10; 1.
DR   Pfam; PF07977; FabA; 1.
DR   SUPFAM; SSF54637; SSF54637; 1.
DR   TIGRFAMs; TIGR01749; fabA; 1.
PE   3: Inferred from homology;
DR   PRODOM; B8IB31.
DR   SWISS-2DPAGE; B8IB31.
KW   Complete proteome {ECO:0000313|Proteomes:UP000008207};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00405,
KW   ECO:0000256|SAAS:SAAS00699091};
KW   Fatty acid biosynthesis {ECO:0000256|HAMAP-Rule:MF_00405,
KW   ECO:0000256|SAAS:SAAS00699069};
KW   Fatty acid metabolism {ECO:0000256|HAMAP-Rule:MF_00405,
KW   ECO:0000256|SAAS:SAAS00699062};
KW   Isomerase {ECO:0000256|HAMAP-Rule:MF_00405,
KW   ECO:0000256|SAAS:SAAS00699061};
KW   Lipid biosynthesis {ECO:0000256|HAMAP-Rule:MF_00405,
KW   ECO:0000256|SAAS:SAAS00699087};
KW   Lipid metabolism {ECO:0000256|HAMAP-Rule:MF_00405,
KW   ECO:0000256|SAAS:SAAS00699099};
KW   Lyase {ECO:0000256|HAMAP-Rule:MF_00405, ECO:0000256|SAAS:SAAS00699094,
KW   ECO:0000313|EMBL:ACL55424.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008207}.
FT   DOMAIN       38    167       FabA. {ECO:0000259|Pfam:PF07977}.
FT   ACT_SITE     80     80       {ECO:0000256|HAMAP-Rule:MF_00405}.
SQ   SEQUENCE   183 AA;  19811 MW;  2C573C537A6FDB17 CRC64;
     MSADTPATDA QTRPSQFSYE DLLACGRGEM FGPGNAQLPL PPMLMFDRIV SIGRDGGAHG
     KGHVRAELDV RPDLWFFPCH FQGDPVMPGC LGLDALWQMV GFFLGWLGSP GRGRALGVGE
     VKFTDQVLPS VRKVVYGVDL KRVRQGRLVL GIADGWLEAD GRRIYEAQDL RVGLFQAAAP
     AGG
//

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